Rabbit anti-Human, Mouse HuR/ELAVL1 Polyclonal Antibody | anti-HuR/ELAVL1 antibody

HuR/ELAVL1 Rabbit pAb

Cat. Num. AAA4757780

• Gene Names: ELAVL1; HUR; Hua; MelG; ELAV1
• Reactivity: Human, Mouse
• Applications: ELISA, Western Blot, Immunohistochemistry
• Purity: Affinity purified
• Synonyms: HuR/ELAVL1; Polyclonal Antibody; HuR/ELAVL1 Rabbit pAb; ELAV like RNA binding protein 1; HUR; Hua; MelG; ELAV1; anti-HuR/ELAVL1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: Ubiquitous. Detected in brain, liver, thymus and muscle.
• Purity/Purification: Affinity purified
• Form/Format: pH7.4 PBS, 0.05% NaN3, 40% Glycerol

• Applicable Applications for anti-HuR/ELAVL1 antibody: ELISA (EIA), Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P)
• Application Notes: WB: 1:500-1:2000; ELISA: 1:2000-1:5000; IHC: 1:50-1:200
• Immunogen: Fusion protein of human ELAVL1
• Conjugation: Unconjugated
• Molecular Function: RNA-binding protein that binds to the 3'-UTR region of mRNAs and increases their stability (PubMed:14517288, PubMed:18285462). Involved in embryonic stem cells (ESCs) differentiation: preferentially binds mRNAs that are not methylated by N6-methyladenosine (m6A), stabilizing them, promoting ESCs differentiation. Binds to poly-U elements and AU-rich elements (AREs) in the 3'-UTR of target mRNAs (PubMed:8626503, PubMed:17632515, PubMed:18285462, PubMed:23519412, PubMed:14731398). Binds avidly to the AU-rich element in FOS and IL3/interleukin-3 mRNAs. In the case of the FOS AU-rich element, binds to a core element of 27 nucleotides that contain AUUUA, AUUUUA, and AUUUUUA motifs. Binds preferentially to the 5'-UUUU[AG]UUU-3' motif in vitro (PubMed:8626503). With ZNF385A, binds the 3'-UTR of p53/TP53 mRNA to control their nuclear export induced by CDKN2A. Hence, may regulate p53/TP53 expression and mediate in part the CDKN2A anti-proliferative activity. May also bind with ZNF385A the CCNB1 mRNA. Increases the stability of the leptin mRNA harboring an AU-rich element (ARE) in its 3' UTR (PubMed:29180010).
• Modification: Unmodified
• Preparation and Storage: Store at -20 degree C. Stable for 12 months from date of receipt.

Western Blot (WB)

(Gel: 10%SDS-PAGE, Lysate: 40 ug, Lane 1-5: 293T cells, A431 cells, NIH/3T3 cells, hela cells, hepG2 cells, Primary antibody: 820589(ELAVL1 Antibody) at dilution 1/667, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 5 seconds)

Immunohistochemistry (IHC)

(The image is immunohistochemistry of paraffin-embedded Human lung cancer tissue using 820589(ELAVL1 Antibody) at dilution 1/40. (Original magnification: ×200))

Immunohistochemistry (IHC)

(The image is immunohistochemistry of paraffin-embedded Human brain tissue using 820589(ELAVL1 Antibody) at dilution 1/40. (Original magnification: ×200))

Related Product Information for anti-HuR/ELAVL1 antibody

The protein encoded by this gene is a member of the ELAVL family of RNA-binding proteins that contain several RNA recognition motifs, and selectively bind AU-rich elements (AREs) found in the 3' untranslated regions of mRNAs. AREs signal degradation of mRNAs as a means to regulate gene expression, thus by binding AREs, the ELAVL family of proteins play a role in stabilizing ARE-containing mRNAs. This gene has been implicated in a variety of biological processes and has been linked to a number of diseases, including cancer. It is highly expressed in many cancers, and could be potentially useful in cancer identification, prognosis, and therapy.

Product Categories/Family for anti-HuR/ELAVL1 antibody

Primary antibody

NCBI and Uniprot Product Information

• NCBI GI #: 20981691
• NCBI GeneID: 1994
• UniProt Accession #: Q15717; Q53XN6; Q9BTT1; B4DVB8
• Molecular Weight: 326
• NCBI Official Full Name: ELAV-like protein 1
• NCBI Official Synonym Full Names: ELAV like RNA binding protein 1
• NCBI Official Symbol: ELAVL1
• NCBI Official Synonym Symbols: HUR; Hua; MelG; ELAV1
• NCBI Protein Information: ELAV-like protein 1; Hu antigen R; hu-antigen R; embryonic lethal, abnormal vision, drosophila, homolog-like 1; ELAV (embryonic lethal, abnormal vision, Drosophila)-like 1 (Hu antigen R)
• UniProt Protein Name: ELAV-like protein 1
• UniProt Gene Name: ELAVL1
• UniProt Synonym Gene Names: HUR; HuR
• UniProt Entry Name: ELAV1_HUMAN

NCBI Description

The protein encoded by this gene is a member of the ELAVL family of RNA-binding proteins that contain several RNA recognition motifs, and selectively bind AU-rich elements (AREs) found in the 3' untranslated regions of mRNAs. AREs signal degradation of mRNAs as a means to regulate gene expression, thus by binding AREs, the ELAVL family of proteins play a role in stabilizing ARE-containing mRNAs. This gene has been implicated in a variety of biological processes and has been linked to a number of diseases, including cancer. It is highly expressed in many cancers, and could be potentially useful in cancer diagnosis, prognosis, and therapy. [provided by RefSeq, Sep 2012]

Uniprot Description

HuR: Involved in 3'-UTR ARE-mediated MYC stabilization. Binds avidly to the AU-rich element in FOS and IL3/interleukin-3 mRNAs. In the case of the FOS AU-rich element, HUR binds to a core element of 27 nucleotides that contain AUUUA, AUUUUA and AUUUUUA motifs. Binds preferentially to the 5'-UUUU[AG]UUU-3' motif in vitro. Identified in a mRNP complex, at least composed of DHX9, DDX3X, ELAVL1, HNRNPU, IGF2BP1, ILF3, PABPC1, PCBP2, PTBP2, STAU1, STAU2, SYNCRIP and YBX1. Interacts with ANP32A, EIF2C1 and EIF2C2. Ubiquitous. Belongs to the RRM elav family.

Protein type: RNA-binding

Chromosomal Location of Human Ortholog: 19p13.2

Cellular Component: nucleoplasm; membrane; cytoplasm; cytosol; nucleus

Molecular Function: mRNA binding; protein binding; mRNA 3'-UTR binding; RNA binding; double-stranded RNA binding; nucleotide binding; AU-rich element binding; protein kinase binding

Biological Process: positive regulation of translation; multicellular organismal development; mRNA stabilization; gene expression