Immunofluorescence (IF)
(Immunofluorescence analysis of HUVEC cells, using HTR5B Antibody. The picture on the right is blocked with the synthesized peptide.)
Rabbit anti-Mouse, Rat HTR5B Polyclonal Antibody | anti-HTR5B antibody
HTR5B Antibody
Gene Names
Htr5b; 5Ht5b; REC17
Reactivity
Mouse, Rat
Applications
Western Blot, Immunofluorescence, ELISA
Purity
Purified from rabbit antiserum by affinity-chromatography using immunogen.
Synonyms
HTR5B; Polyclonal Antibody; HTR5B Antibody; 5-hydroxytryptamine receptor 5B; 5-HT-5B; Serotonin receptor 5B; MR22; Htr5b; 5ht5b; anti-HTR5B antibody
Host
Rabbit
Reactivity
Mouse, Rat
Clonality
Polyclonal
Specificity
HTR5B Antibody detects endogenous levels of total HTR5B protein.
Purity/Purification
Purified from rabbit antiserum by affinity-chromatography using immunogen.
Form/Format
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1 mg/ml (varies by lot)
Sequence Length
370
Applicable Applications for anti-HTR5B antibody
Western Blot (WB), Immunofluorescence (IF), ELISA (EIA)
Application Notes
WB: 1:500~1:1000
IF: 1:100~1:500
ELISA: 1:40000
IF: 1:100~1:500
ELISA: 1:40000
Target Modification
Unmodified/Total
Immunogen
The antiserum was produced against synthesized peptide derived from rat HTR5B.
Immunogen Range
280-329
Preparation and Storage
Stable at -20 degree C for at least 1 year.
Immunofluorescence (IF)
(Immunofluorescence analysis of HUVEC cells, using HTR5B Antibody. The picture on the right is blocked with the synthesized peptide.)
Immunofluorescence (IF)
(Immunofluorescence analysis of HUVEC cells, using HTR5B Antibody. The picture on the right is blocked with the synthesized peptide.)
Western Blot (WB)
(Western blot analysis of lysates from COLO cells, using HTR5B Antibody. The lane on the right is blocked with the synthesized peptide.)
Western Blot (WB)
(Western blot analysis of lysates from COLO cells, using HTR5B Antibody. The lane on the right is blocked with the synthesized peptide.)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
41 kDa
NCBI Official Full Name
5-hydroxytryptamine receptor 5B
NCBI Official Synonym Full Names
5-hydroxytryptamine (serotonin) receptor 5B
NCBI Official Symbol
Htr5b
NCBI Official Synonym Symbols
5Ht5b; REC17
NCBI Protein Information
5-hydroxytryptamine receptor 5B
UniProt Protein Name
5-hydroxytryptamine receptor 5B
Protein Family
UniProt Gene Name
Htr5b
UniProt Synonym Gene Names
5ht5b; 5-HT-5B; 5-HT5B
NCBI Description
G-protein coupled receptor for serotonin [RGD, Feb 2006]
Uniprot Description
This is one of the several different receptors for 5-hydroxytryptamine (serotonin), a biogenic hormone that functions as a neurotransmitter, a hormone, and a mitogen. The activity of this receptor is mediated by G proteins. Probably involved in anxiety and depression.
Research Articles on HTR5B
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Product Notes
The HTR5B htr5b (Catalog #AAA9507872) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The HTR5B Antibody reacts with Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's HTR5B can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunofluorescence (IF), ELISA (EIA). WB: 1:500~1:1000 IF: 1:100~1:500 ELISA: 1:40000. Researchers should empirically determine the suitability of the HTR5B htr5b for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "HTR5B, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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