Goat HTATSF1 Polyclonal Antibody | anti-HTATSF1 antibody

Goat anti-HTATSF1 Antibody

Cat. Num. AAA420880

• Gene Names: HTATSF1; TATSF1; TAT-SF1; dJ196E23.2
• Reactivity: Tested: Human; Expected from sequence similarity: Human, Rat, Dog
• Applications: ELISA, Western Blot
• Purity: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Synonyms: HTATSF1; Polyclonal Antibody; Goat anti-HTATSF1 Antibody; HIV-1 Tat specific factor 1; RP1-196E23.2; TAT-SF1; dJ196E23.2; HIV TAT specific factor 1; cofactor required for Tat activation of HIV-1 transcription; HTATSF1 antibody; HIV-1 Tat specific factor 1 antibody; RP1-196E23.2 antibody; TAT-SF1 antibody; dJ196E23.2 antibody; HIV TAT specific factor 1 antibody; cofactor required for Tat activation of HIV-1 transcription antibody; anti-HTATSF1 antibody

• Host: Goat
• Reactivity: Tested: Human; Expected from sequence similarity: Human, Rat, Dog
• Clonality: Polyclonal
• Purity/Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Form/Format: Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
• Concentration: 100ug specific antibody in 200ul (varies by lot)
• Sequence: QELYGDGKDGDTQTD
• Sequence Length: 755

• Applicable Applications for anti-HTATSF1 antibody: Peptide ELISA (EIA), Western Blot (WB)
• Application Notes: Peptide ELISA: Antibody detection limit dilution 1: 16000.
• Immunogen: Peptide with sequence C-QELYGDGKDGDTQTD, from the internal region (near the N Terminus) of the protein sequence according to NP_055315.2.
• Epitope: Internal region (near the N Terminus)
• Preparation and Storage: Aliquot and store at -20 degree C. Minimize freezing and thawing.

Western Blot (WB)

((1ug/ml) staining of Daudi cell lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)

NCBI and Uniprot Product Information

• NCBI GI #: 21361437
• NCBI GeneID: 27336
• NCBI Accession #: NP_055315.2
• NCBI GenBank Nucleotide #: NM_014500.4
• Molecular Weight: 85,853 Da
• NCBI Official Full Name: HIV Tat-specific factor 1
• NCBI Official Synonym Full Names: HIV-1 Tat specific factor 1
• NCBI Official Symbol: HTATSF1
• NCBI Official Synonym Symbols: TATSF1; TAT-SF1; dJ196E23.2
• NCBI Protein Information: HIV Tat-specific factor 1
• UniProt Protein Name: HIV Tat-specific factor 1
• Protein Family: HIV Tat-specific factor
• UniProt Gene Name: HTATSF1
• UniProt Synonym Gene Names: Tat-SF1
• UniProt Entry Name: HTSF1_HUMAN

NCBI Description

The protein encoded by this gene functions as a cofactor for the stimulation of transcriptional elongation by HIV-1 Tat, which binds to the HIV-1 promoter through Tat-TAR interaction. This protein may also serve as a dual-function factor to couple transcription and splicing and to facilitate their reciprocal activation. Alternatively spliced transcript variants have been found for this gene.[provided by RefSeq, Sep 2009]

Uniprot Description

TAT-SF1: Functions as a general transcription factor playing a role in the process of transcriptional elongation. May mediate the reciprocal stimulatory effect of splicing on transcriptional elongation. In case of infection by HIV-1, it is up-regulated by the HIV-1 proteins NEF and gp120, acts as a cofactor required for the Tat-enhanced transcription of the virus. Belongs to the HTATSF1 family.

Protein type: Transcription, coactivator/corepressor; Spliceosome

Chromosomal Location of Human Ortholog: Xq26.3

Cellular Component: nucleoplasm; nucleus; snRNP U2

Molecular Function: nucleotide binding; RNA binding

Biological Process: nuclear mRNA splicing, via spliceosome; regulation of RNA elongation; regulation of transcription from RNA polymerase II promoter; transcription, DNA-dependent; viral genome replication