Rabbit anti-Human HSP90AB1 Polyclonal Antibody | anti-HSP90AB1 antibody

HSP90AB1, CT (HSP90AB1, HSP90B, HSPC2, HSPCB, Heat shock protein HSP 90-beta, Heat shock 84kD)

Cat. Num. AAA646480

• Gene Names: HSP90AB1; HSP84; HSPC2; HSPCB; D6S182; HSP90B
• Reactivity: Human
• Applications: ELISA, Western Blot, Immunohistochemistry, Flow Cytometry, Functional Assay
• Purity: Purified; Purified by saturated ammonium sulfate precipitation followed by dialysis against PBS.
• Synonyms: HSP90AB1; Polyclonal Antibody; CT (HSP90AB1; HSP90B; HSPC2; HSPCB; Heat shock protein HSP 90-beta; Heat shock 84kD); Anti -HSP90AB1; anti-HSP90AB1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Human
• Purity/Purification: Purified; Purified by saturated ammonium sulfate precipitation followed by dialysis against PBS.
• Form/Format: Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.

• Applicable Applications for anti-HSP90AB1 antibody: ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS)
• Application Notes: Suitable for use in Western Blot, Immunohistochemistry, Flow Cytometry, ELISA; Dilution: ELISA: 1:1,000; Western Blot: 1:50-100; Immunohistochemistry: 1:50-100; Flow Cytometry: 1:10-50
• Immunogen: HSP90AB1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 695~724 amino acids from the C-terminal region of human HSP90AB1.
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western blot analysis of HSP90AB1 Antibody (C-term) in HL-60 cell line lysates (35ug/lane). HSP90AB1 (arrow) was detected using the purified Pab.)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human testis tissue reacted with HSP90AB1 Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)

Flow Cytometry (FC/FACS)

(HSP90AB1 Antibody (C-term) flow cytometric analysis of HL-60 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Related Product Information for anti-HSP90AB1 antibody

HSPCB are highly conserved molecular chaperones that have key roles in signal transduction, protein folding, protein degradation, and morphologic evolution. This protein normally associate with other cochaperones and play important roles in folding newly synthesized proteins or stabilizing and refolding denatured proteins after stress.

Product Categories/Family for anti-HSP90AB1 antibody

Antibodies; Abs to Heat Shock Proteins

NCBI and Uniprot Product Information

• NCBI GI #: 431822408
• NCBI GeneID: 3326
• NCBI Accession #: NP_001258901.1
• NCBI GenBank Nucleotide #: NM_001271972.1
• UniProt Accession #: P08238; Q5T9W7; Q9NQW0; Q9NTK6; B2R5P0
• Molecular Weight: 83,264 Da
• NCBI Official Full Name: heat shock protein HSP 90-beta isoform c
• NCBI Official Synonym Full Names: heat shock protein 90kDa alpha (cytosolic), class B member 1
• NCBI Official Symbol: HSP90AB1
• NCBI Official Synonym Symbols: HSP84; HSPC2; HSPCB; D6S182; HSP90B
• NCBI Protein Information: heat shock protein HSP 90-beta; HSP90-beta; heat shock 84 kDa; heat shock 90kD protein 1, beta
• UniProt Protein Name: Heat shock protein HSP 90-beta
• Protein Family: Heat shock protein
• UniProt Gene Name: HSP90AB1
• UniProt Synonym Gene Names: HSP90B; HSPC2; HSPCB; HSP 90; HSP 84; HSP84
• UniProt Entry Name: HS90B_HUMAN

NCBI Description

This gene encodes a member of the heat shock protein 90 family; these proteins are involved in signal transduction, protein folding and degradation and morphological evolution. This gene encodes the constitutive form of the cytosolic 90 kDa heat-shock protein and is thought to play a role in gastric apoptosis and inflammation. Alternative splicing results in multiple transcript variants. Pseudogenes have been identified on multiple chromosomes. [provided by RefSeq, Dec 2012]

Uniprot Description

Function: Molecular chaperone that promotes the maturation, structural maintenance and proper regulation of specific target proteins involved for instance in cell cycle control and signal transduction. Undergoes a functional cycle that is linked to its ATPase activity. This cycle probably induces conformational changes in the client proteins, thereby causing their activation. Interacts dynamically with various co-chaperones that modulate its substrate recognition, ATPase cycle and chaperone function. Ref.22 Ref.29

Subunit structure: Homodimer. Interacts with p53/TP53

By similarity. Forms a complex with CDK6 and Hsp90/HSP90AB1. Interacts with UNC45A. Binding to UNC45A involves 2 UNC45A monomers per HSP90AB1 dimer. Interacts with CHORDC1 and DNAJC7

By similarity. Interacts with FKBP4. Ref.17 Ref.22 Ref.25

Subcellular location: Cytoplasm. Melanosome. Note: Identified by mass spectrometry in melanosome fractions from stage I to stage IV. Ref.17 Ref.21

Domain: The TPR repeat-binding motif mediates interaction with TPR repeat-containing proteins

By similarity.

Post-translational modification: Ubiquitinated in the presence of STUB1-UBE2D1 complex (in vitro). Ref.24ISGylated. Ref.18S-nitrosylated; negatively regulates the ATPase activity

Probable.

Sequence similarities: Belongs to the heat shock protein 90 family.

Sequence caution: The sequence AAD14062.3 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.The sequence CAB66478.1 differs from that shown. Reason: Frameshift at position 709.