Rabbit HSP90AA1/Hsp90 Alpha A1 Polyclonal Antibody | anti-HSP90AA1 antibody

Anti-HSP90AA1/Hsp90 Alpha A1 Antibody

Cat. Num. AAA2400915

• Gene Names: HSP90AA1; EL52; HSPN; LAP2; HSP86; HSPC1; HSPCA; Hsp89; Hsp90; LAP-2; HSP89A; HSP90A; HSP90N; Hsp103; HSPCAL1; HSPCAL4; HEL-S-65p
• Reactivity: Human; Predicted: Mouse, Rat
• Applications: ELISA, Immunohistochemistry, Western Blot
• Purity: Protein A purified
• Synonyms: HSP90AA1/Hsp90 Alpha A1; Polyclonal Antibody; Anti-HSP90AA1/Hsp90 Alpha A1 Antibody; Rabbit Polyclonal (IgG) to Human HSP90AA1/Hsp90 Alpha A1; HSP90AA1; EL52; HSP86; Hsp89; HSP89A; HSPC1; Heat shock 86 kDa; Hsp90; HSP90A; HSP90N; HSPCA; HSPCAL4; HSPN; LAP2; HSP 86; HSPCAL1; anti-HSP90AA1 antibody

• Host: Rabbit
• Reactivity: Human; Predicted: Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: While ELISA data show strong reactivity with the acetylated form of the immunizing peptide and minimal reactivity with the non-acetylated form, to date western blotting data are not definitive for acetyl K294 specificity as blots show equivalent staining of lysates from cells either treated or untreated with Trichostatin A (an HDAC inhibitor). A BLAST analysis was used to suggest cross-reactivity with Hsp90 from human, mouse, rat, monkey, chicken and Drosophila based on 100% homology with the immunizing sequence. Reactivity of this antibody with Hsp90 from other species is unknown.
• Purity/Purification: Protein A purified
• Form/Format: 0.02 M potassium phosphate, 0.15 M sodium chloride, pH 7.2, 0.01% sodium azide, sterile filtered
• Sequence Length: 732

• Applicable Applications for anti-HSP90AA1 antibody: ELISA (EIA), Immunohistochemistry (IHC) Paraffin, Western Blot (WB)
• Application Notes: IHC-P: 5 ug/ml; WB: 1:500-1:2500
• Immunogen: This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to amino acids surrounding K294 of human Hsp90.
• Conjugation: Unconjugated
• Preparation and Storage: Short Term: Store at 4 degree C; Long Term: Aliquot and store at -20 degree C; Avoid freeze thaw cycles.

Immunohistochemistry (IHC)

(Anti-HSP90AA1/Hsp90 antibody IHC staining of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.)

Immunohistochemistry (WB)

(Anti-HSP90AA1/Hsp90 antibody IHC staining of human heart. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.)

Western Blot (WB)

(Western Blot - Anti-Hsp90 Acetyl K29 Antibody. Western blot of Affinity Purified anti-Hsp90 acetyl K294 antibody shows detection of a band at ~90 kD corresponding to Hsp90 in an SkBr3 cell lysate (arrowhead) after treatment with Trichostatin A (an HDAC inhibitor). Western blotting results do not definitively demonstrate the acetyl K294 specificity of this reagent because similar staining is seen in the control lane (no treatment with TSA). Immunoprecipitation with anti-Hsp90 was performed prior to western blotting with anti-Hsp90 acetyl K294.)

Related Product Information for anti-HSP90AA1 antibody

Molecular chaperone that promotes the maturation, structuralmaintenance and proper regulation of specific target proteins involvedfor instance in cell cycle control and signal transduction. Undergoes afunctional cycle that is linked to its ATPase activity. This cycleprobably induces conformational changes in the client proteins, thereby causing their activation. Interacts dynamically with variousco-chaperones that modulate its substrate recognition, ATPase cycle and chaperone function.

NCBI and Uniprot Product Information

• NCBI GI #: 154146191
• NCBI GeneID: 3320
• NCBI Accession #: NP_005339.3
• NCBI GenBank Nucleotide #: NM_005348.3
• UniProt Accession #: P07900; Q2PP14; Q5CAQ6; Q5CAQ7; Q9BVQ5; A8K500; B3KPJ9
• Molecular Weight: 98,161 Da
• NCBI Official Full Name: heat shock protein HSP 90-alpha isoform 2
• NCBI Official Synonym Full Names: heat shock protein 90 alpha family class A member 1
• NCBI Official Symbol: HSP90AA1
• NCBI Official Synonym Symbols: EL52; HSPN; LAP2; HSP86; HSPC1; HSPCA; Hsp89; Hsp90; LAP-2; HSP89A; HSP90A; HSP90N; Hsp103; HSPCAL1; HSPCAL4; HEL-S-65p
• NCBI Protein Information: heat shock protein HSP 90-alpha
• UniProt Protein Name: Heat shock protein HSP 90-alpha
• Protein Family: Heat shock protein
• UniProt Gene Name: HSP90AA1
• UniProt Synonym Gene Names: HSP90A; HSPC1; HSPCA; HSP 86; HSP86; LAP-2; LPS-associated protein 2

NCBI Description

The protein encoded by this gene is an inducible molecular chaperone that functions as a homodimer. The encoded protein aids in the proper folding of specific target proteins by use of an ATPase activity that is modulated by co-chaperones. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jan 2012]

Uniprot Description

Molecular chaperone that promotes the maturation, structural maintenance and proper regulation of specific target proteins involved for instance in cell cycle control and signal transduction. Undergoes a functional cycle that is linked to its ATPase activity which is essential for its chaperone activity. This cycle probably induces conformational changes in the client proteins, thereby causing their activation. Interacts dynamically with various co-chaperones that modulate its substrate recognition, ATPase cycle and chaperone function (PubMed:11274138, PubMed:15577939, PubMed:15937123, PubMed:27353360, PubMed:29127155). Engages with a range of client protein classes via its interaction with various co-chaperone proteins or complexes, that act as adapters, simultaneously able to interact with the specific client and the central chaperone itself (PubMed:29127155). Recruitment of ATP and co-chaperone followed by client protein forms a functional chaperone. After the completion of the chaperoning process, properly folded client protein and co-chaperone leave HSP90 in an ADP-bound partially open conformation and finally, ADP is released from HSP90 which acquires an open conformation for the next cycle (PubMed:27295069, PubMed:26991466). Apart from its chaperone activity, it also plays a role in the regulation of the transcription machinery. HSP90 and its co-chaperones modulate transcription at least at three different levels (PubMed:25973397). In the first place, they alter the steady-state levels of certain transcription factors in response to various physiological cues(PubMed:25973397). Second, they modulate the activity of certain epigenetic modifiers, such as histone deacetylases or DNA methyl transferases, and thereby respond to the change in the environment (PubMed:25973397). Third, they participate in the eviction of histones from the promoter region of certain genes and thereby turn on gene expression (PubMed:25973397). Binds bacterial lipopolysaccharide (LPS) and mediates LPS-induced inflammatory response, including TNF secretion by monocytes (PubMed:11276205). Antagonizes STUB1-mediated inhibition of TGF-beta signaling via inhibition of STUB1-mediated SMAD3 ubiquitination and degradation (PubMed:24613385).