Rabbit anti-Human, Mouse HSP60 Polyclonal Antibody | anti-HSP60 antibody

HSP60, NT (Heat Shock 70kD Protein 14, Heat Shock Protein HSP60, HSP70-like Protein 1, HSPA14, HSP70L1) (HRP)

Cat. Num. AAA6479744

• Gene Names: HSPA14; HSP70-4; HSP70L1
• Reactivity: Human, Mouse
• Applications: Immunohistochemistry, ELISA, Western Blot
• Purity: Purified by Ammonium Sulfate Precipitation.
• Synonyms: HSP60; Polyclonal Antibody; NT (Heat Shock 70kD Protein 14; Heat Shock Protein HSP60; HSP70-like Protein 1; HSPA14; HSP70L1) (HRP); anti-HSP60 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human Hsp60. Species Crossreactivity: mouse.
• Purity/Purification: Purified by Ammonium Sulfate Precipitation.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).

• Applicable Applications for anti-HSP60 antibody: Immunohistochemistry (IHC), ELISA (EIA), Western Blot (WB)
• Application Notes: IHC: 1:50-1:100; ELISA: 1:1,000; WB: 1:50-1:100; Applications are based on unconjugated antibody.
• Immunogen: Synthetic peptide selected from the N-terminal region of human Hsp60 (KLH).
• Conjugate: HRP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates.

Western Blot (WB)

(Western blot analysis of Hsp 60 Antibody (N-term) in mouse lung tissue lysates (35ug/lane). Hsp (arrow) was detected using the purified Pab.)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human lung carcinoma with Hsp 60 Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)

Product Categories/Family for anti-HSP60 antibody

Antibodies; Heat Shock Proteins

References

1. Velez, D.R., et.al., Am. J. Obstet. Gynecol. 200(2), 209 (2009).

NCBI and Uniprot Product Information

• NCBI GI #: 82880658
• NCBI GeneID: 51182
• NCBI Accession #: NP_057383
• NCBI GenBank Nucleotide #: NM_016299
• UniProt Accession #: Q0VDF9
• Molecular Weight: 56kDa
• NCBI Official Full Name: heat shock 70 kDa protein 14 isoform 1
• NCBI Official Synonym Full Names: heat shock protein family A (Hsp70) member 14
• NCBI Official Symbol: HSPA14
• NCBI Official Synonym Symbols: HSP70-4; HSP70L1
• NCBI Protein Information: heat shock 70 kDa protein 14
• UniProt Protein Name: Heat shock 70 kDa protein 14
• Protein Family: Hydrogenosomal chaperonin
• UniProt Gene Name: HSPA14
• UniProt Synonym Gene Names: HSP60; HSP70L1
• UniProt Entry Name: HSP7E_HUMAN

Uniprot Description

HSPA14: Component of the ribosome-associated complex (RAC), a complex involved in folding or maintaining nascent polypeptides in a folding-competent state. In the RAC complex, binds to the nascent polypeptide chain, while DNAJC2 stimulates its ATPase activity. Belongs to the heat shock protein 70 family.

Protein type: Heat shock protein

Chromosomal Location of Human Ortholog: 10p13

Cellular Component: membrane; ribosome; cytosol

Molecular Function: protein binding; ATP binding

Biological Process: 'de novo' cotranslational protein folding