Rabbit Hsp27 Polyclonal Antibody | anti-HSPB1 antibody

Hsp27, phosphorylated (Ser15)

Cat. Num. AAA615790

• Gene Names: HSPB1; CMT2F; HMN2B; HSP27; HSP28; Hsp25; SRP27; HS.76067; DKFZp586P1322
• Reactivity: Bovine, Canine, Guinea Pig, Human, Monkey
• Applications: Western Blot
• Purity: Affinity Purified; Purified by immunoaffinity chromatography.
• Synonyms: Hsp27; Polyclonal Antibody; phosphorylated (Ser15); Anti -Hsp27; anti-HSPB1 antibody

• Host: Rabbit
• Reactivity: Bovine, Canine, Guinea Pig, Human, Monkey
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Specific for phosphorylated Hsp27 (Ser15) at ~27kD protein, corresponding to the MR of phosphorylated Hsp27 (Ser15) on SDS-PAGE immunoblots. Does not crossreact with non-phosphorylated Hsp27. Species Crossreactivity: Human, mouse, rat, bovine, canine, guinea pig and monkey.
• Purity/Purification: Affinity Purified; Purified by immunoaffinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2, 0.02% sodium azide, before the addition of glycerol to 40%.

• Applicable Applications for anti-HSPB1 antibody: Western Blot (WB)
• Application Notes: Dilution: Western Blot (Colorimetric): 0.1ug/ml detects phosphorylated Hsp27 in 20ug MCF-7 cell lysate.; Optimal dilutions to be determined by researcher.
• Immunogen: Human Hsp27 phosphorylated synthetic peptide, corresponding to aa10-20 (KLH coupled).
• Preparation and Storage: May be stored at 4 degree C for short-term only. For long-term storage, store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Related Product Information for anti-HSPB1 antibody

Human Hsp27 belongs to the phylogenically conserved small heat shock protein (smHsp) family, which includes mouse Hsp25, alphaA-crystallins and alphaB-crystallins. Members of this protein superfamily from the animal, plant and microbiotic kingdoms share the so-called alpha-crystallin domain of approximately 90 amino acid residues which is bounded by variable N-and C-terminal extensions (1). Hsp27 is expressed constitutively in many tissues and its expression is increased to high levels after various types of stress including elevated temperatures, toxic metals, drugs and oxidants. Hsp27 is believed to exist mainly as oligomers of as many as 8-40 of Hsp27 protein monomers in cells and data suggest that these large oligomers have a chaperone-like activity by serving as a site where unfolding proteins may bind until ATP and Hsp70-dependent refolding can occur (2). Hsp27 is phosphorylated on three phosphorylation sites (Ser15, Ser78 and Ser82) by protein kinases including MAPKAP kinase 2/3 and the stress-activated protein kinase SAPK2 (p38) (3,4). Studies on cells stimulated by a variety of mitogenic and stress factors suggest that phosphorylation of Hsp27 occurs as an early prominent event and that phosphorylation induced changes in the ultrastructure of Hsp27 may regulate its biochemical activities. The state of phosphorylation and oligomerization of Hsp27 may regulate microfilament organization as shown by studies demonstrating that only the nonphosphorylated lower molecular weight forms of Hsp27 bind actin barbed ends and inhibit polymerization (5). Hsp27 is believed to protect cells by enhancing cellular glutathione levels as indicated by elevated glutathione levels in cells overexpressing Hsp27. Studies using wild-type Hsp27 and mutants in which the serine phosphorylation sites were mutated to alanines, glycines or aspartates, demonstrate that cellular glutathione levels depend on the oligomerization of Hsp27 with only the large oligomeric forms of Hsp27 capable of protecting cells by enhancing glutathione levels (6).

Product Categories/Family for anti-HSPB1 antibody

Antibodies; Abs to Heat Shock Proteins

NCBI and Uniprot Product Information

• NCBI GI #: 11036357
• NCBI GeneID: 3315
• UniProt Accession #: P04792; Q6FI47; Q96C20; Q96EI7; Q9UC31; Q9UC34; Q9UC35; Q9UC36; B2R4N8
• Molecular Weight: 22,783 Da
• NCBI Official Full Name: HSP27
• NCBI Official Synonym Full Names: heat shock 27kDa protein 1
• NCBI Official Symbol: HSPB1
• NCBI Official Synonym Symbols: CMT2F; HMN2B; HSP27; HSP28; Hsp25; SRP27; HS.76067; DKFZp586P1322
• NCBI Protein Information: heat shock protein beta-1; HSP 27; OTTHUMP00000161096; 28 kDa heat shock protein; heat shock 27 kDa protein; heat shock 27kD protein 1; stress-responsive protein 27; estrogen-regulated 24 kDa protein
• UniProt Protein Name: Heat shock protein beta-1
• Protein Family: Heat shock protein
• UniProt Gene Name: HSPB1
• UniProt Synonym Gene Names: HSP27; HSP28
• UniProt Entry Name: HSPB1_HUMAN

NCBI Description

The protein encoded by this gene is induced by environmental stress and developmental changes. The encoded protein is involved in stress resistance and actin organization and translocates from the cytoplasm to the nucleus upon stress induction. Defects in this gene are a cause of Charcot-Marie-Tooth disease type 2F (CMT2F) and distal hereditary motor neuropathy (dHMN). [provided by RefSeq]

Uniprot Description

HSP27: a small heat shock protein that is regulated both transcriptionally and posttranslationally. Modulates actin polymerization and reorganization. Its expression level increases several-fold in response to stress and is phosphorylated by MAPKAP kinase 2. Cytoplasmic in interphase cells. Colocalizes with mitotic spindles in mitotic cells. Translocates to the nucleus during heat shock.

Protein type: Chaperone; Motility/polarity/chemotaxis; Heat shock protein

Chromosomal Location of Human Ortholog: 7q11.23

Cellular Component: proteasome complex; extracellular space; cytoskeleton; focal adhesion; cytoplasm; plasma membrane; spindle; Z disc; cytosol; nucleus

Molecular Function: protein kinase C inhibitor activity; identical protein binding; protein binding; ubiquitin binding; protein kinase C binding; protein kinase binding

Biological Process: positive regulation of tumor necrosis factor biosynthetic process; response to virus; positive regulation of blood vessel endothelial cell migration; response to unfolded protein; retinal homeostasis; positive regulation of interleukin-1 beta production; positive regulation of angiogenesis; negative regulation of protein kinase activity; gene expression; cell motility; vascular endothelial growth factor receptor signaling pathway; regulation of I-kappaB kinase/NF-kappaB cascade; regulation of translational initiation; negative regulation of apoptosis

Disease: Neuronopathy, Distal Hereditary Motor, Type Iib; Charcot-marie-tooth Disease, Axonal, Type 2f