Immunofluorescence (IF)
(Immunofluorescence staining of methanol-fixed Hela cells showing cytoplasmic, nuclear, centrosomal, midbody staining using HSP27 (Phospho-Ser78) antibody)
Rabbit HSP27 Polyclonal Antibody | anti-HSP27 antibody
HSP27 antibody
Applications
Immunofluorescence, Immunohistochemistry, Western Blot
Purity
Purified by immunoaffinity chromatography.
Synonyms
HSP27; Polyclonal Antibody; HSP27 antibody; Rabbit polyclonal HSP27 antibody; Polyclonal HSP27 antibody; Anti-HSP27 antibody; HSP 27; HSP-27; HSP 27 antibody; HSP-27 antibody; Heat Shock Protein 25 antibody; Heat Shock Protein 27 antibody; Phospho Ser78 antibody; HSP-25 antibody; HSP 25 antibody; HSP25 antibody; anti-HSP27 antibody
Host
Rabbit
Clonality
Polyclonal
Purity/Purification
Purified by immunoaffinity chromatography.
Form/Format
Liquid form in PBS buffer (no MG2+ or Ca2+), pH 7.4, 150 mm NaCl,0.02% sodium azide and 50% glycerol.
Concentration
1 mg/ml (varies by lot)
Applicable Applications for anti-HSP27 antibody
Immunofluorescence (IF), Immunohistochemistry (IHC), Western Blot (WB)
Application Notes
IF: 1:100-1:200
IHC: 1:50-1:100
WB: 1:500-1:1000
IHC: 1:50-1:100
WB: 1:500-1:1000
Immunogen
HSP27 antibody was raised in Rabbit using the peptide sequence around phosphorylation site of serine 78 (A-L-S(p)-R-Q) derived from Human HSP27 as the immunogen.
Cross-Reactivity
Human
Preparation and Storage
Store at 4 degree C for short term storage.
Aliquot and store at-20 degree C for long term storage.
Avoid repeated freeze/thaw cycles.
Aliquot and store at-20 degree C for long term storage.
Avoid repeated freeze/thaw cycles.
Immunofluorescence (IF)
(Immunofluorescence staining of methanol-fixed Hela cells showing cytoplasmic, nuclear, centrosomal, midbody staining using HSP27 (Phospho-Ser78) antibody)
Immunofluorescence (IF)
(Immunofluorescence staining of methanol-fixed Hela cells showing cytoplasmic, nuclear, centrosomal, midbody staining using HSP27 (Phospho-Ser78) antibody)
Western Blot (WB)
(Western Blot analysis of extracts from HL60 cells untreated or treated with UV using HSP27 (Phospho-Ser78) antibody)
Western Blot (WB)
(Western Blot analysis of extracts from HL60 cells untreated or treated with UV using HSP27 (Phospho-Ser78) antibody)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using HSP27 (Phospho-Ser78) antibody (left) or the same antibody preincubated with with blocking peptide (right))
Immunohistochemistry (IHC)
(Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using HSP27 (Phospho-Ser78) antibody (left) or the same antibody preincubated with with blocking peptide (right))
Related Product Information for anti-HSP27 antibody
Heat shock proteins (HSP) are a class of functionally related proteins whose expression is increased when cells are exposed to elevated temperatures or other stress. This increase in expression is transcriptionally regulated. HSPs are found in virtually all living organisms, from bacteria to humans and are named according to their molecular weight.
Product Categories/Family for anti-HSP27 antibody
Similar Products
Product Notes
The HSP27 (Catalog #AAA5309592) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's HSP27 can be used in a range of immunoassay formats including, but not limited to, Immunofluorescence (IF), Immunohistochemistry (IHC), Western Blot (WB). IF: 1:100-1:200 IHC: 1:50-1:100 WB: 1:500-1:1000. Researchers should empirically determine the suitability of the HSP27 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "HSP27, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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