Immunohistochemistry (IHC)
(Immunohistochemistry analysis using Rabbit Anti-HSP110 Polyclonal Antibody (SPC-195). Tissue: backskin. Species: Mouse. Fixation: Bouin's Fixative Solution. Primary Antibody: Rabbit Anti-HSP110 Polyclonal Antibody (SPC-195) at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Rabbit (green) at 1:50 for 1 hour at RT. Localization: Positive epidermal, dermal and muscle staining.)
Rabbit Hsp110 Polyclonal Antibody | anti-HSPH1 antibody
Hsp110 Antibody: ATTO 390
Gene Names
HSPH1; HSP105; HSP105A; HSP105B; NY-CO-25
Reactivity
Human, Monkey, Hamster, Rat, Mouse, Cow, Sheep, Yeast
Applications
Western Blot
Synonyms
Hsp110; Polyclonal Antibody; Hsp110 Antibody: ATTO 390; Heat shock 105kDa alpha; heat shock 105kDa beta; Hsp105; Hsp105A; Hsp105B; HSPH1; NY CO 25; anti-HSPH1 antibody
Host
Rabbit
Reactivity
Human, Monkey, Hamster, Rat, Mouse, Cow, Sheep, Yeast
Clonality
Polyclonal
Form/Format
Affinity Purified
Concentration
1mg/mL (varies by lot)
Sequence Length
442
Applicable Applications for anti-HSPH1 antibody
Western Blot (WB)
Application Notes
1:1000 (WB)
Conjugate
ATTO 390
Storage Buffer
PBS pH7.2, 50% glycerol, 0.09% sodium azide
Preparation and Storage
-20 degree C
Immunohistochemistry (IHC)
(Immunohistochemistry analysis using Rabbit Anti-HSP110 Polyclonal Antibody (SPC-195). Tissue: backskin. Species: Mouse. Fixation: Bouin's Fixative Solution. Primary Antibody: Rabbit Anti-HSP110 Polyclonal Antibody (SPC-195) at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Rabbit (green) at 1:50 for 1 hour at RT. Localization: Positive epidermal, dermal and muscle staining.)
Immunohistochemistry (IHC)
(Immunohistochemistry analysis using Rabbit Anti-HSP110 Polyclonal Antibody (SPC-195). Tissue: backskin. Species: Mouse. Fixation: Bouin's Fixative Solution. Primary Antibody: Rabbit Anti-HSP110 Polyclonal Antibody (SPC-195) at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Rabbit (green) at 1:50 for 1 hour at RT. Localization: Positive epidermal, dermal and muscle staining.)
Western Blot (WB)
(Western blot analysis of Human Cell line lysates showing detection of HSP110 protein using Rabbit Anti-HSP110 Polyclonal Antibody (SPC-195). Load: 15 ugprotein. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Rabbit Anti-HSP110 Polyclonal Antibody (SPC-195) at 1:1000 for 2 hours at RT. Secondary Antibody: Donkey Anti-Rabbit IgG: HRP for 1 hour at RT.)
Western Blot (WB)
(Western blot analysis of Human Cell line lysates showing detection of HSP110 protein using Rabbit Anti-HSP110 Polyclonal Antibody (SPC-195). Load: 15 ugprotein. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Rabbit Anti-HSP110 Polyclonal Antibody (SPC-195) at 1:1000 for 2 hours at RT. Secondary Antibody: Donkey Anti-Rabbit IgG: HRP for 1 hour at RT.)
Related Product Information for anti-HSPH1 antibody
Background Info: Detects ~110kDa
Scientific Background: Hsp110 belongs to a family of large stress proteins known as the Hsp110/SSE Family. The proteins in this family are the most distantly known relatives of the well studied Hsp70 family. They share 30-33% amino acid identity, mostly in the conserved ATP-binding domain (1). Hsp110 cooperates with Hsp70 in protein folding in the eukaryotic cytosol (2). In mammals, Hsp110 is constitutively expressed, but exhibits particularly high levels in the brain. Both Hsp70 and Hsp110 are elevated after cerebral ischemia. Recent studies demonstrate that the protective effects of Hsp110 deficiency in cerebral ischemia may partly be mediated by an increase in the chaperone activity of Hsp70 (3). Studies also suggest that Hsp110 can be used in heat shock protein-based cancer immunotherapy (4).
Scientific Background: Hsp110 belongs to a family of large stress proteins known as the Hsp110/SSE Family. The proteins in this family are the most distantly known relatives of the well studied Hsp70 family. They share 30-33% amino acid identity, mostly in the conserved ATP-binding domain (1). Hsp110 cooperates with Hsp70 in protein folding in the eukaryotic cytosol (2). In mammals, Hsp110 is constitutively expressed, but exhibits particularly high levels in the brain. Both Hsp70 and Hsp110 are elevated after cerebral ischemia. Recent studies demonstrate that the protective effects of Hsp110 deficiency in cerebral ischemia may partly be mediated by an increase in the chaperone activity of Hsp70 (3). Studies also suggest that Hsp110 can be used in heat shock protein-based cancer immunotherapy (4).
Product Categories/Family for anti-HSPH1 antibody
References
1. Lee-Yoon D., et al. (1995) J Biol Chem. 270: 15725-15733. 2. Dragovic Z., et al. (2006) EMBO J. 25: 2519- 2528. 3. Nakamura J., et al. (2008) Stroke. 39(10): 2853-2859. 4. Subject D.R. (2001) Health Research Inc. Report number AS90893: 1-84.
NCBI and Uniprot Product Information
NCBI GeneID
Molecular Weight
51,178 Da
NCBI Official Synonym Full Names
heat shock 105kDa/110kDa protein 1
NCBI Official Symbol
HSPH1
NCBI Official Synonym Symbols
HSP105; HSP105A; HSP105B; NY-CO-25
NCBI Protein Information
heat shock protein 105 kDa
UniProt Protein Name
Septin-8
Protein Family
UniProt Gene Name
SEPT8
UniProt Synonym Gene Names
KIAA0202
UniProt Entry Name
SEPT8_HUMAN
Uniprot Description
SEPT8: Filament-forming cytoskeletal GTPase. May play a role in cytokinesis (Potential). May play a role in platelet secretion. Belongs to the septin family. 3 isoforms of the human protein are produced by alternative splicing.
Protein type: Hydrolase; Cytoskeletal
Chromosomal Location of Human Ortholog: 5q31
Cellular Component: cytoskeleton; cytoplasm
Molecular Function: protein binding; GTP binding
Biological Process: cell cycle
Research Articles on HSPH1
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Product Notes
The HSPH1 sept8 (Catalog #AAA806380) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Hsp110 Antibody: ATTO 390 reacts with Human, Monkey, Hamster, Rat, Mouse, Cow, Sheep, Yeast and may cross-react with other species as described in the data sheet. AAA Biotech's Hsp110 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). 1:1000 (WB). Researchers should empirically determine the suitability of the HSPH1 sept8 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Hsp110, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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