Western Blot (WB)
(Western Blot (WB) analysis of HSD3B2 polyclonal antibody at 1:500 dilutionLane1:HEK293T whole cell lysateLane2:Raw264. 7 whole cell lysateLane3:H9C2 whole cell lysate)
Rabbit HSD3B2 Polyclonal Antibody | anti-HSD3B2 antibody
HSD3B2 Polyclonal Antibody
Gene Names
HSD3B2; HSDB; HSD3B; SDR11E2
Reactivity
Human, Mouse, Rat
Applications
Western Blot
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
Synonyms
HSD3B2; Polyclonal Antibody; HSD3B2 Polyclonal Antibody; 3beta-HSD; HSD-3beta; 3 beta-hydroxysteroid dehydrogenase/Delta 5-->4-isomerase type 2; 3 beta-hydroxysteroid dehydrogenase/Delta 5-->4-isomerase type II; 3-beta-HSD II; 3-beta-HSD adrenal and gonadal type; 3-beta-hydroxy-Delta(5)-steroid dehydrogenase; 3-beta-hydroxy-5-ene steroid dehydrogenase Progesterone reductase; Steroid Delta-isomerase; Delta-5-3-ketosteroid isomerase; HSDB3B; anti-HSD3B2 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Specificity
HSD3B2 polyclonal antibody detects endogenous levels of HSD3B2 protein.
Purity/Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
Form/Format
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Sequence Length
372
Applicable Applications for anti-HSD3B2 antibody
Western Blot (WB)
Application Notes
WB: 1:500-1:1000
Immunogen
A synthetic peptide corresponding to residues in Human HSD3B2.
Preparation and Storage
Store at 4 degree C short term.
Aliquot and store at -20 degree C long term.
Avoid freeze-thaw cycles.
Aliquot and store at -20 degree C long term.
Avoid freeze-thaw cycles.
Western Blot (WB)
(Western Blot (WB) analysis of HSD3B2 polyclonal antibody at 1:500 dilutionLane1:HEK293T whole cell lysateLane2:Raw264. 7 whole cell lysateLane3:H9C2 whole cell lysate)
Western Blot (WB)
(Western Blot (WB) analysis of HSD3B2 polyclonal antibody at 1:500 dilutionLane1:HEK293T whole cell lysateLane2:Raw264. 7 whole cell lysateLane3:H9C2 whole cell lysate)
Related Product Information for anti-HSD3B2 antibody
3 beta-hydroxysteroid dehydrogenase (3beta-HSD), also known as HSD3B1 or HSDB3, is a bifunctional enzyme that plays a crucial role in the synthesis of all classes of hormonal steroids. Two human 3beta-HSD proteins, designated type I (3beta-HSD) and type II (3beta-HSD2), are expressed by different genes and function in different areas of the body. Localized to the membrane of the endoplasmic reticulum (ER) and expressed in skin and placenta, 3beta-HSD is the type I protein that catalyzes the oxidative conversion of delta5-ene-3-beta-hydroxy steroid, as well as the conversion of various ketosteroids. Defects in the gene encoding 3beta-HSD are associated with classic salt wasting, genital ambiguity, hypogonadism, insulin-resistant polycystic ovary syndrome (PCOS) and an increased susceptibility to prostate cancer. Additionally, congenital deficiency of 3beta-HSD activity results in a severe depletion of steroid formation which can be lethal in young children.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
UniProt Accession #
Molecular Weight
~ 42kDa
NCBI Official Full Name
HSD3B2 protein
NCBI Official Synonym Full Names
hydroxy-delta-5-steroid dehydrogenase, 3 beta- and steroid delta-isomerase 2
NCBI Official Symbol
HSD3B2
NCBI Official Synonym Symbols
HSDB; HSD3B; SDR11E2
NCBI Protein Information
3 beta-hydroxysteroid dehydrogenase/Delta 5-->4-isomerase type 2
UniProt Protein Name
3 beta-hydroxysteroid dehydrogenase/Delta 5-->4-isomerase type 2
Protein Family
UniProt Gene Name
HSD3B2
UniProt Synonym Gene Names
HSDB3B; 3-beta-HSD II
UniProt Entry Name
3BHS2_HUMAN
NCBI Description
The protein encoded by this gene is a bifunctional enzyme that catalyzes the oxidative conversion of delta(5)-ene-3-beta-hydroxy steroid, and the oxidative conversion of ketosteroids. It plays a crucial role in the biosynthesis of all classes of hormonal steroids. This gene is predominantly expressed in the adrenals and the gonads. Mutations in this gene are associated with 3-beta-hydroxysteroid dehydrogenase, type II, deficiency. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Oct 2009]
Research Articles on HSD3B2
Similar Products
Product Notes
The HSD3B2 hsd3b2 (Catalog #AAA3006366) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The HSD3B2 Polyclonal Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's HSD3B2 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). WB: 1:500-1:1000. Researchers should empirically determine the suitability of the HSD3B2 hsd3b2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "HSD3B2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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