Western Blot (WB)
((0.3ug/ml) staining of Mouse Lung lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Goat Hsd11b1 Polyclonal Antibody | anti-Hsd11b1 antibody
Goat anti-Hsd11b1 (mouse) Antibody
Western Blot: Approx 32kDa band observed in Mouse Lung lysates (calculated MW of 32.4kDa according to NP_032314.2). Recommended concentration: 0.3-1ug/ml. An additional band of unknown identity was also consistently observed at 45kDa. This band was successfully blocked by incubation with the immunizing peptide. However, it remained visible in a KO background.
Western Blot (WB)
((0.3ug/ml) staining of Mouse Lung lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Western Blot (WB)
((0.3ug/ml) staining of Mouse Lung lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Western Blot (WB)
((0.3ug/ml) staining of Mouse Lung lysate (60ug protein in RIPA buffer). First lane shows wildtype and second lane shows knockout background. Primary incubation was overnight. Detected by fluorescence with Li-cor). Data obtained from Prof. K. Chapman and Zhenguang Zhang, Centre for Cardiovascular Sciences, Queen's Medical Research Institute, Edinburgh, UK)
Western Blot (WB)
((0.3ug/ml) staining of Mouse Lung lysate (60ug protein in RIPA buffer). First lane shows wildtype and second lane shows knockout background. Primary incubation was overnight. Detected by fluorescence with Li-cor). Data obtained from Prof. K. Chapman and Zhenguang Zhang, Centre for Cardiovascular Sciences, Queen's Medical Research Institute, Edinburgh, UK)
NCBI and Uniprot Product Information
Uniprot Description
HSD11B1: Catalyzes reversibly the conversion of cortisol to the inactive metabolite cortisone. Catalyzes reversibly the conversion of 7-ketocholesterol to 7-beta-hydroxycholesterol. In intact cells, the reaction runs only in one direction, from 7- ketocholesterol to 7-beta-hydroxycholesterol. Defects in HSD11B1 are a cause of cortisone reductase deficiency (CRD). In CRD, activation of cortisone to cortisol does not occur, resulting in adrenocorticotropin-mediated androgen excess and a phenotype resembling polycystic ovary syndrome (PCOS). Belongs to the short-chain dehydrogenases/reductases (SDR) family.
Protein type: Endoplasmic reticulum; Oxidoreductase; Lipid Metabolism - androgen and estrogen; Lipid Metabolism - C21-steroid hormone; Membrane protein, integral; EC 1.1.1.146
Cellular Component: apical part of cell; cytoplasm; endoplasmic reticulum; integral to membrane; intracellular membrane-bound organelle; membrane; nuclear membrane; rough endoplasmic reticulum
Molecular Function: 11-beta-hydroxysteroid dehydrogenase activity; NADP binding; oxidoreductase activity; steroid binding
Biological Process: glucocorticoid biosynthetic process; glucocorticoid catabolic process; lipid metabolic process; lung development; regulation of pentose-phosphate shunt; steroid metabolic process
Research Articles on Hsd11b1
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Product Notes
The Hsd11b1 hsd11b1 (Catalog #AAA422747) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The Goat anti-Hsd11b1 (mouse) Antibody reacts with Tested: Mouse; Expected from sequence similarity: Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's Hsd11b1 can be used in a range of immunoassay formats including, but not limited to, Peptide ELISA (EIA), Western Blot (WB). Peptide ELISA: Antibody detection limit dilution 1: 32000. Western Blot: Approx 32kDa band observed in Mouse Lung lysates (calculated MW of 32.4kDa according to NP_032314.2). Recommended concentration: 0.3-1ug/ml. An additional band of unknown identity was also consistently observed at 45kDa. This band was successfully blocked by incubation with the immunizing peptide. However, it remained visible in a KO background. Researchers should empirically determine the suitability of the Hsd11b1 hsd11b1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: TARSEEGLQK VVSR. It is sometimes possible for the material contained within the vial of "Hsd11b1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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