Rabbit anti-Human, Mouse HSC70 Interacting Protein Polyclonal Antibody | anti-HIP antibody

HSC70 Interacting Protein (HIP, AAG2, FAM10A1, FAM10A4, FLJ27260, HOP, HSPABP, HSPABP1, MGC129952, P48, PRO0786, Progesterone Receptor-associated p48 Protein, Protein FAM10A1, Putative Tumor Suppressor ST13, Renal Carcinoma Antigen NY-REN-33, SNC6, ST13,

Cat. Num. AAA6481185

• Gene Names: ST13; HIP; HOP; P48; AAG2; SNC6; HSPABP; FAM10A1; FAM10A4; HSPABP1; PRO0786
• Reactivity: Human, Mouse
• Applications: Western Blot
• Purity: Purified by Protein G Affinity Chromatography.
• Synonyms: HSC70 Interacting Protein; Polyclonal Antibody; HSC70 Interacting Protein (HIP; AAG2; FAM10A1; FAM10A4; FLJ27260; HOP; HSPABP; HSPABP1; MGC129952; P48; PRO0786; Progesterone Receptor-associated p48 Protein; Protein FAM10A1; Putative Tumor Suppressor ST13; Renal Carcinoma Antigen NY-REN-33; SNC6; ST13; ; Hsc70-interacting Protein; OTTHUMP00000028873; anti-HIP antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human ST13. Species Crossreactivity: mouse.
• Purity/Purification: Purified by Protein G Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Allophycocyanin (APC).

• Applicable Applications for anti-HIP antibody: Western Blot (WB), FLISA
• Application Notes: WB: 1:100-1:500; FLISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: Synthetic peptide selected from the N-terminal region of human ST13 (KLH).
• Conjugate: APC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Do not freeze APC conjugates. Light sensitive. Aliquots are stable for at least 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human colon carcinoma tissue reacted with ST13 antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)

Western Blot (WB)

(Western blot analysis of anti-ST13 Antibody (N-term) pre-incubated with and without blocking peptide (BP6247a) in A2058 cell line lysate. ST13(arrow) was detected using the purified Pab.)

Product Categories/Family for anti-HIP antibody

Antibodies; Heat Shock Proteins

References

1. Rajapandi, T., et al., J. Biol. Chem. 275(29):22597-22604 (2000). 2. Morishima, Y., et al., J. Biol. Chem. 275(10):6894-6900 (2000). 3. Chen, S., et al., J. Biol. Chem. 273(52):35194-35200 (1998). 4. Johnson, B.D., et al., J. Biol. Chem. 273(6):3679-3686 (1998). 5. Cao, J., et al., J. Cancer Res. Clin. Oncol. 123(8):447-451 (1997).

NCBI and Uniprot Product Information

• NCBI GI #: 19923193
• NCBI GeneID: 6767
• NCBI Accession #: NP_003923
• NCBI GenBank Nucleotide #: NM_003932.4
• Molecular Weight: 41,332 Da
• NCBI Official Full Name: hsc70-interacting protein isoform 1
• NCBI Official Synonym Full Names: suppression of tumorigenicity 13 (colon carcinoma) (Hsp70 interacting protein)
• NCBI Official Symbol: ST13
• NCBI Official Synonym Symbols: HIP; HOP; P48; AAG2; SNC6; HSPABP; FAM10A1; FAM10A4; HSPABP1; PRO0786
• NCBI Protein Information: hsc70-interacting protein; Hsp70-interacting protein; aging-associated protein 2; heat shock 70kD protein binding protein; progesterone receptor-associated p48 protein; putative tumor suppressor ST13; renal carcinoma antigen NY-REN-33; suppression of tumo
• Protein Family: HSP-interacting protein

NCBI Description

The protein encoded by this gene is an adaptor protein that mediates the association of the heat shock proteins HSP70 and HSP90. This protein has been shown to be involved in the assembly process of glucocorticoid receptor, which requires the assistance of multiple molecular chaperones. The expression of this gene is reported to be downregulated in colorectal carcinoma tissue suggesting that it is a candidate tumor suppressor gene. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jun 2013]