Rabbit Hsc70 (Hsp73) Polyclonal Antibody | anti-HSPA8 antibody

Hsc70 (Hsp73) Antibody

Cat. Num. AAA805128

• Gene Names: HSPA8; LAP1; HSC54; HSC70; HSC71; HSP71; HSP73; LAP-1; NIP71; HEL-33; HSPA10; HEL-S-72p
• Reactivity: Human, Hamster, Rat
• Applications: Western Blot, Immunoprecipitation, Immunofluorescence, Immunohistochemistry
• Synonyms: Hsc70 (Hsp73); Polyclonal Antibody; Hsc70 (Hsp73) Antibody; Hsc54; Hsc71; Hsc73; Hsp71; Hsp73; HspA10; HspA8; LAP1; NIP71; anti-HSPA8 antibody

• Host: Rabbit
• Reactivity: Human, Hamster, Rat
• Clonality: Polyclonal
• Form/Format: Rabbit Antiserum
• Sequence Length: 646

• Applicable Applications for anti-HSPA8 antibody: Western Blot (WB), Immunoprecipitation (IP), Immunofluorescence (IF), Immunohistochemistry (IHC), Blocking
• Application Notes: 1: 1500-2,000 (WB); 1:250 (IF)
• Certificate of Analysis: 0.5 ug/mL of SPC-102 was sufficient for detection of Hsc70 20ug of heat shocked HeLa cell lysate by colorimetric immunoblot analysis using Goat anti-rabbit IgG:HRP as the secondary antibody.
• Storage Buffer: Rabbit antiserum
• Preparation and Storage: -20 degree C

Immunohistochemistry (IHC)

(Immunohistochemistry analysis using Rabbit Anti-HSC70 Polyclonal Antibody (SPC-102). Tissue: backskin. Species: Mouse. Fixation: Bouin's Fixative Solution. Primary Antibody: Rabbit Anti-HSC70 Polyclonal Antibody (SPC-102) at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Rabbit (green) at 1:50 for 1 hour at RT. Localization: Cytoplasm.)

Immunohistochemistry (IHC)

(Immunohistochemistry analysis using Rabbit Anti-HSC70 Polyclonal Antibody (SPC-102). Tissue: colon carcinoma. Species: Human. Fixation: Formalin. Primary Antibody: Rabbit Anti-HSC70 Polyclonal Antibody (SPC-102) at 1:10000 for 12 hours at 4 degree C. Secondary Antibody: Biotin Goat Anti-Rabbit at 1:2000 for 1 hour at RT. Counterstain: Methyl Green at 200uL for 2 min at RT.)

Western Blot (WB)

(Western blot analysis of Human Cell line lysates showing detection of HSC70 protein using Rabbit Anti-HSC70 Polyclonal Antibody (SPC-102). Load: 15 ugprotein. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Rabbit Anti-HSC70 Polyclonal Antibody (SPC-102) at 1:1000 for 2 hours at RT. Secondary Antibody: Donkey Anti-Rabbit IgG: HRP for 1 hour at RT.)

Immunohistochemistry (IHC)

(Immunohistochemistry analysis using Rabbit Anti-HSC70 Polyclonal Antibody (SPC-102). Tissue: Inflamed colon. Species: Mouse. Fixation: Formalin. Primary Antibody: Rabbit Anti-HSC70 Polyclonal Antibody (SPC-102) at 1:50000 for 12 hours at 4 degree C. Secondary Antibody: Biotin Goat Anti-Rabbit at 1:2000 for 1 hour at RT. Counterstain: Methyl Green at 200uL for 2 min at RT.)

Related Product Information for anti-HSPA8 antibody

Background Info: Detects 73kDa protein corresponding to the molecular mass of hsc70 on SDS PAGE immunoblots. Does not cross-react with hsp70.

Scientific Background: Hsp70 genes encode abundant heat-inducible 70-kDa hsps Hsp70 genes encode abundant heat-inducible 70-kDa hsps

Product Categories/Family for anti-HSPA8 antibody

Chaperones; Heat Shock; Trafficking

References

1. Brown C.L. et al. (1993) J.Cell Biol., 120 (5): 1101-1112. 2. Boorstein W.R., Ziegelhoffer T., and Craig E.A. (1993) J. Mol. Evol. 38(1): 1-17. 3. Rothman J. (1989), Cell 59: 591-601. 4. DeLuca-Flaherty et al. (1990) Cell 62: 875-887. 5. Bork P., Sander C., and Valencia A. (1992) Proc. Nut1 Acad. Sci. USA 89: 7290-7294. 6. Brown C.L. et al. (1996) J. Biol. Chem. 271(2): 833- 840.
1. Hatakeyama, T et. al. (2013) Connexin43 Functions as a Novel Interacting Partner of Heat Shock Cognate Protein 70. Scientific Reports 3 : 2719. doi: 10.1038/srep02719
2. Gong, H., et al. (2013). Characterization and binding analysis of a microneme adhesive repeat domain-containing protein from Toxoplasma gondii. Parasitology International. doi: 10.1016/j.parint.2013.12.006
3. Zhang, D. et al. (2012). Ouabain mimics low temperature rescue of F508del-CFTR in cystic fibrosis epithelial cells. Front. Pharmacol. 3 (176). doi: 10.3389/fphar.2012.00176
4. Zwang, N.A. et al. (2009). Identification of Phosphorylation-Dependent Binding Partners of Aquaporin-2 Using Protein Mass Spectrometry. J. Proteome Res. 8 (3), 1540-1554. doi: 10.1021/pr800894p

NCBI and Uniprot Product Information

• NCBI GI #: 5729877
• NCBI GeneID: 3312
• NCBI Accession #: NP_006588.1
• NCBI GenBank Nucleotide #: NM_006597.5
• UniProt Accession #: P11142; Q9H3R6
• Molecular Weight: 53,518 Da
• NCBI Official Full Name: heat shock cognate 71 kDa protein isoform 1
• NCBI Official Synonym Full Names: heat shock 70kDa protein 8
• NCBI Official Symbol: HSPA8
• NCBI Official Synonym Symbols: LAP1; HSC54; HSC70; HSC71; HSP71; HSP73; LAP-1; NIP71; HEL-33; HSPA10; HEL-S-72p
• NCBI Protein Information: heat shock cognate 71 kDa protein; LPS-associated protein 1; heat shock 70kd protein 10; epididymis luminal protein 33; heat shock cognate protein 54; constitutive heat shock protein 70; lipopolysaccharide-associated protein 1; N-myristoyltransferase inhibitor protein 71; epididymis secretory sperm binding protein Li 72p
• UniProt Protein Name: Heat shock cognate 71 kDa protein
• UniProt Gene Name: HSPA8
• UniProt Synonym Gene Names: HSC70; HSP73; HSPA10; LAP-1; LPS-associated protein 1
• UniProt Entry Name: HSP7C_HUMAN

NCBI Description

This gene encodes a member of the heat shock protein 70 family, which contains both heat-inducible and constitutively expressed members. This protein belongs to the latter group, which are also referred to as heat-shock cognate proteins. It functions as a chaperone, and binds to nascent polypeptides to facilitate correct folding. It also functions as an ATPase in the disassembly of clathrin-coated vesicles during transport of membrane components through the cell. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Aug 2011]

Uniprot Description

HSC70: Acts as a repressor of transcriptional activation. Inhibits the transcriptional coactivator activity of CITED1 on Smad-mediated transcription. Chaperone. Isoform 2 may function as an endogenous inhibitory regulator of HSC70 by competing the co- chaperones. Interacts with HSPH1/HSP105. Interacts with IRAK1BP1. Identified in a mRNP granule complex, at least composed of ACTB, ACTN4, DHX9, ERG, HNRNPA1, HNRNPA2B1, HNRNPAB, HNRNPD, HNRNPL, HNRNPR, HNRNPU, HSPA1, HSPA8, IGF2BP1, ILF2, ILF3, NCBP1, NCL, PABPC1, PABPC4, PABPN1, RPLP0, RPS3, RPS3A, RPS4X, RPS8, RPS9, SYNCRIP, TROVE2, YBX1 and untranslated mRNAs. Interacts with PACRG and TSC2. Interacts with BAG1. Interacts with SV40 VP1. Interacts with DNAJC7. Interacts with HERC5. Interacts with CITED1 (via N-terminus); the interaction suppresses the association of CITED1 to p300/CBP and Smad-mediated transcription transactivation. Constitutively synthesized. Ubiquitous. Belongs to the heat shock protein 70 family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Nucleolus; Heat shock protein; Chaperone; RNA-binding

Chromosomal Location of Human Ortholog: 11q24.1

Cellular Component: extracellular space; focal adhesion; ribonucleoprotein complex; cytosol; nucleoplasm; spliceosome; membrane; melanosome; plasma membrane; nucleolus; intracellular; nucleus; ubiquitin ligase complex

Molecular Function: protein binding; enzyme binding; G-protein-coupled receptor binding; heat shock protein binding; ubiquitin protein ligase binding; ATPase activity; unfolded protein binding; ATPase activity, coupled; ATP binding

Biological Process: axon guidance; chaperone cofactor-dependent protein folding; viral reproduction; transcription, DNA-dependent; protein folding; regulation of cell cycle; neurotransmitter secretion; RNA splicing; response to unfolded protein; positive regulation of nuclear mRNA splicing, via spliceosome; nuclear mRNA splicing, via spliceosome; synaptic transmission; protein refolding; gene expression; post-Golgi vesicle-mediated transport; negative regulation of transcription, DNA-dependent