Sheep anti-Human HS6ST1 Polyclonal Antibody | anti-HS6ST1 antibody

HS6ST1 (Heparan-sulfate 6-O-sulfotransferase 1, DKFZp547H098, FLJ25392, Heparan-sulfate 6-O-sulfotransferase 1, HS6ST, HS6ST-1, MGC116899, MGC116901)

Cat. Num. AAA6009962

• Gene Names: HS6ST1; HH15; HS6ST
• Reactivity: Human
• Applications: Western Blot
• Purity: Affinity Purified; Purified by immunoaffinity chromatography.
• Synonyms: HS6ST1; Polyclonal Antibody; HS6ST1 (Heparan-sulfate 6-O-sulfotransferase 1; DKFZp547H098; FLJ25392; Heparan-sulfate 6-O-sulfotransferase 1; HS6ST; HS6ST-1; MGC116899; MGC116901); Anti -HS6ST1 (Heparan-sulfate 6-O-sulfotransferase 1; anti-HS6ST1 antibody

• Host: Sheep
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human.
• Purity/Purification: Affinity Purified; Purified by immunoaffinity chromatography.
• Form/Format: Supplied as a lyophilized powder in PBS, with Trehalose. Reconstitute with sterile PBS.

• Applicable Applications for anti-HS6ST1 antibody: Western Blot (WB)
• Application Notes: Suitable for use in Western Blot.; Dilution: Western Blot: 1mg/ml
• Immunogen: Recombinant corresponding to human HS6ST1 expressed in CHO cells (AAY14736).
• Preparation and Storage: Lyophilized powder may be stored at -20 degree C. Stable for 12 months at -20 degree C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Reconstituted product is stable for 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Related Product Information for anti-HS6ST1 antibody

Heparan Sulfate is a highly sulfated polysaccharide that can be found on cell surface and extrracellular matrix.It is usually covalently attached to a protein core as the glycan component of a proteoglycan. Heparan Sulfate interacts with a variety of proteins,such as growth factors, protease inhibitors, cytokines, lipoprotein lipase and viral envelope proteins, thus plays roles from cell growth, cell differentiation, cell motility, blood coagulation, lipid metabolism to viral infection (1,2).Heparan Sulfate consists of repeating residues of uronic acid and N-acetyl glucosamine. The uronic acid residues can be sulfated at 2-O position by Heparan Sulfate 2-0 Sulfotransferase (HS2ST). The N-acetyl glucosamine residues can be sulfated at N-, 3-O, and 6-O positions by N-deacetylase/N-sulfotransferases (NDSTs), Heparan Sulfate 3-O and 6-O sulfotransferases (HS3STs and HS6STs) respectively. However, the reactions catalyzed by these sulfotransferases are normally incomplete on the whole chain of Heparan Sulfate. As a result, Heparan Sulfate displays enormous sequence diversity that allows it to interact with a wide spect rum of proteins differently. Among three HS6STs, HS6ST1is the first to be cloned(3).Mice deficient of the HS6ST1 homologue gene showed embryonic lethality(4).

Product Categories/Family for anti-HS6ST1 antibody

Antibodies; Abs to Enzymes; Sulfotransferase

NCBI and Uniprot Product Information

• NCBI GI #: 148747866
• NCBI GeneID: 9394
• NCBI Accession #: NP_004798.3
• NCBI GenBank Nucleotide #: NM_004807.2
• UniProt Accession #: O60243; Q53SL2; Q9BVI1; B4DEP2; B4DJ29
• Molecular Weight: 48,226 Da
• NCBI Official Full Name: heparan-sulfate 6-O-sulfotransferase 1
• NCBI Official Synonym Full Names: heparan sulfate 6-O-sulfotransferase 1
• NCBI Official Symbol: HS6ST1
• NCBI Official Synonym Symbols: HH15; HS6ST
• NCBI Protein Information: heparan-sulfate 6-O-sulfotransferase 1; HS6ST-1; heparan-sulfate 6-sulfotransferase
• UniProt Protein Name: Heparan-sulfate 6-O-sulfotransferase 1
• Protein Family: Heparan-sulfate 6-O-sulfotransferase
• UniProt Gene Name: HS6ST1
• UniProt Synonym Gene Names: HS6ST; HS6ST-1
• UniProt Entry Name: H6ST1_HUMAN

NCBI Description

The protein encoded by this gene is a member of the heparan sulfate biosynthetic enzyme family. Heparan sulfate biosynthetic enzymes are key components in generating a myriad of distinct heparan sulfate fine structures that carry out multiple biological activities. This enzyme is a type II integral membrane protein and is responsible for 6-O-sulfation of heparan sulfate. This enzyme does not share significant sequence similarity with other known sulfotransferases. A pseudogene located on chromosome 1 has been found for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

HS6ST1: 6-O-sulfation enzyme which catalyzes the transfer of sulfate from 3'-phosphoadenosine 5'-phosphosulfate (PAPS) to position 6 of the N-sulfoglucosamine residue (GlcNS) of heparan sulfate. Belongs to the sulfotransferase 6 family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral; Transferase; EC 2.8.2.-; Glycan Metabolism - heparan sulfate biosynthesis

Chromosomal Location of Human Ortholog: 2q21

Cellular Component: Golgi membrane; integral to plasma membrane

Molecular Function: sulfotransferase activity; heparan sulfate 6-O-sulfotransferase activity

Biological Process: glycosaminoglycan biosynthetic process; glycosaminoglycan metabolic process; heparan sulfate proteoglycan biosynthetic process, enzymatic modification; carbohydrate metabolic process; neuron development; pathogenesis; angiogenesis; alveolus development

Disease: Hypogonadotropic Hypogonadism 15 With Or Without Anosmia