Rabbit anti-Human HRD1 Polyclonal Antibody | anti-SYVN1 antibody

HRD1, NT (SYVN1, HRD1, KIAA1810, E3 ubiquitin-protein ligase synoviolin, Synovial apoptosis inhibitor 1)

Cat. Num. AAA648122

• Gene Names: SYVN1; DER3; HRD1
• Reactivity: Human
• Applications: ELISA, Western Blot
• Purity: Purified; Purified by saturated ammonium sulfate precipitation followed by dialysis against PBS.
• Synonyms: HRD1; Polyclonal Antibody; NT (SYVN1; KIAA1810; E3 ubiquitin-protein ligase synoviolin; Synovial apoptosis inhibitor 1); Anti -HRD1; anti-SYVN1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Human
• Purity/Purification: Purified; Purified by saturated ammonium sulfate precipitation followed by dialysis against PBS.
• Form/Format: Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.

• Applicable Applications for anti-SYVN1 antibody: ELISA (EL/EIA), Western Blot (WB)
• Application Notes: Suitable for use in Western Blot, ELISA; Dilution: ELISA: 1:1,000; Western Blot: 1:50-100
• Immunogen: HRD1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between amino acid 115-145 from the N-terminal region of human HRD1.
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western blot analysis of HRD1 Antibody (N-term) in SK-BR-3 cell line lysates (35ug/lane). HRD1 (arrow) was detected using the purified Pab.)

Related Product Information for anti-SYVN1 antibody

HRD1 encodes a protein involved in endoplasmic reticulum (ER)-associated degradation. The encoded protein removes unfolded proteins, accumulated during ER stress, by retrograde transport to the cytosol from the ER. This protein also uses the ubiquitin-proteasome system for additional degradation of unfolded proteins. This gene and the mitochondrial ribosomal protein L49 gene use in their respective 3' UTRs some of the same genomic sequence.

Product Categories/Family for anti-SYVN1 antibody

Antibodies; Abs to Ubiquitin Ligases

NCBI and Uniprot Product Information

• NCBI GI #: 25396434
• NCBI GeneID: 84447
• UniProt Accession #: Q86TM6; Q8N3K3; Q8N6E8; Q96JL5; Q96PK3
• Molecular Weight: 67,685 Da
• NCBI Official Full Name: HRD1
• NCBI Official Synonym Full Names: synovial apoptosis inhibitor 1, synoviolin
• NCBI Official Symbol: SYVN1
• NCBI Official Synonym Symbols: DER3; HRD1
• NCBI Protein Information: E3 ubiquitin-protein ligase synoviolin; HMG-coA reductase degradation 1 homolog
• UniProt Protein Name: E3 ubiquitin-protein ligase synoviolin
• Protein Family: E3 ubiquitin-protein ligase
• UniProt Gene Name: SYVN1
• UniProt Synonym Gene Names: HRD1; KIAA1810
• UniProt Entry Name: SYVN1_HUMAN

NCBI Description

This gene encodes a protein involved in endoplasmic reticulum (ER)-associated degradation. The encoded protein removes unfolded proteins, accumulated during ER stress, by retrograde transport to the cytosol from the ER. This protein also uses the ubiquitin-proteasome system for additional degradation of unfolded proteins. Sequence analysis identified two transcript variants that encode different isoforms. [provided by RefSeq, May 2011]

Uniprot Description

Function: Acts as an E3 ubiquitin-protein ligase which accepts ubiquitin specifically from endoplasmic reticulum-associated UBC7 E2 ligase and transfers it to substrates, promoting their degradation. Component of the endoplasmic reticulum quality control (ERQC) system also called ER-associated degradation (ERAD) involved in ubiquitin-dependent degradation of misfolded endoplasmic reticulum proteins. Also promotes the degradation of normal but naturally short-lived proteins such as SGK. Protects cells from ER stress-induced apoptosis. Protects neurons from apoptosis induced by polyglutamine-expanded huntingtin (HTT) or unfolded GPR37 by promoting their degradation. Sequesters p53/TP53 in the cytoplasm and promotes its degradation, thereby negatively regulating its biological function in transcription, cell cycle regulation and apoptosis. Ref.1 Ref.2 Ref.3 Ref.4 Ref.8 Ref.11 Ref.12 Ref.13 Ref.14 Ref.22

Pathway: Protein modification; protein ubiquitination.

Subunit structure: Homodimer. Interacts with p53/TP53 and HTT. Interacts with VCP, HERPUD1 and DERL1. Part of a complex containing SYVN1, HERPUD1, VIMP and DERL1; which probably transfer misfolded proteins from the ER to VCP. Part of a complex containing SYVN1, SEL1L and DERL2. Interacts with UBXN6. Interacts with SEL1L; recruits ERLEC1 and OS9. May form a complex with ERLEC1; HSPA5; OS9 AND SEL1L. Ref.8 Ref.9 Ref.10 Ref.13 Ref.14 Ref.15 Ref.16 Ref.18

Subcellular location: Endoplasmic reticulum membrane; Multi-pass membrane protein Ref.1 Ref.2 Ref.4 Ref.9.

Tissue specificity: Ubiquitously expressed, with highest levels in liver and kidney (at protein level). Up-regulated in synovial tissues from patients with rheumatoid arthritis (at protein level). Ref.1 Ref.2 Ref.3

Induction: By endoplasmic reticulum stress-inducing agents such as thapsigargin, tunicamycin or brefeldin A, but not by heat shock. Ref.1 Ref.4

Domain: The RING-type zinc finger is required for E3 ligase activity.

Post-translational modification: Not N-glycosylated. Ref.4Auto-ubiquitinated.

Sequence similarities: Belongs to the HRD1 family.Contains 1 RING-type zinc finger.

Sequence caution: The sequence BAB47439.1 differs from that shown. Reason: Erroneous initiation.