Rabbit anti-Human HPRT1 Polyclonal Antibody | anti-HPRT1 antibody

HPRT1, NT (HPRT1, HPRT, Hypoxanthine-guanine phosphoribosyltransferase) (MaxLight 405)

Cat. Num. AAA6308248

• Gene Names: HPRT1; HPRT; HGPRT
• Reactivity: Human
• Applications: Flow Cytometry, Functional Assay, Immunohistochemistry, Western Blot
• Purity: Purified by saturated ammonium sulfate precipitation followed by dialysis against PBS.
• Synonyms: HPRT1; Polyclonal Antibody; NT (HPRT1; HPRT; Hypoxanthine-guanine phosphoribosyltransferase) (MaxLight 405); Hypoxanthine-guanine phosphoribosyltransferase; anti-HPRT1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by saturated ammonium sulfate precipitation followed by dialysis against PBS.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight405.

• Applicable Applications for anti-HPRT1 antibody: Flow Cytometry (FC/FACS), FLISA, Immunohistochemistry (IHC), Western Blot (WB)
• Application Notes: FC/FACS: 1:10-50; FLISA: 1:1,000; IHC: 1:10-50; WB: 1:50-100; Applications are based on unconjugated antibody.
• Immunogen: HPRT1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected between amino acids 65 and 95 of human HPRT1.
• Conjugate: MaxLight405
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight405 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western blot analysis of HPRT1 (arrow) using rabbit polyclonal HPRT1 Antibody (N-term). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the HPRT1 gene.)

Immunohistochemistry (IHC)

(HPRT1 Antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human liver tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of HPRT1 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.)

Flow Cytometry (FC/FACS)

(HPRT1 Antibody (N-term) flow cytometric analysis of 293 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Related Product Information for anti-HPRT1 antibody

The protein is a transferase, which catalyzes conversion of hypoxanthine to inosine monophosphate and guanine to guanosine monophosphate via transfer of the 5-phosphoribosyl group from 5-phosphoribosyl 1-pyrophosphate. This enzyme plays a central role in the generation of purine nucleotides through the purine salvage pathway.

Product Categories/Family for anti-HPRT1 antibody

Antibodies; Enzymes

References

Ceballos-Picot, I., Hum. Mol. Genet. 18 (13), 2317-2327 (2009). Sculley, D.G., Hum. Genet. 90 (3), 195-207 (1992).

NCBI and Uniprot Product Information

• NCBI GI #: 4504483
• NCBI GeneID: 3251
• NCBI Accession #: NP_000185.1
• NCBI GenBank Nucleotide #: NM_000194.2
• UniProt Accession #: P00492; A6NHF0; B2R8M9
• Molecular Weight: 24,579 Da
• NCBI Official Full Name: hypoxanthine-guanine phosphoribosyltransferase
• NCBI Official Synonym Full Names: hypoxanthine phosphoribosyltransferase 1
• NCBI Official Symbol: HPRT1
• NCBI Official Synonym Symbols: HPRT; HGPRT
• NCBI Protein Information: hypoxanthine-guanine phosphoribosyltransferase; HGPRTase
• UniProt Protein Name: Hypoxanthine-guanine phosphoribosyltransferase
• Protein Family: Hypoxanthine-guanine phosphoribosyltransferase
• UniProt Gene Name: HPRT1
• UniProt Synonym Gene Names: HPRT; HGPRT; HGPRTase
• UniProt Entry Name: HPRT_HUMAN

NCBI Description

The protein encoded by this gene is a transferase, which catalyzes conversion of hypoxanthine to inosine monophosphate and guanine to guanosine monophosphate via transfer of the 5-phosphoribosyl group from 5-phosphoribosyl 1-pyrophosphate. This enzyme plays a central role in the generation of purine nucleotides through the purine salvage pathway. Mutations in this gene result in Lesch-Nyhan syndrome or gout.[provided by RefSeq, Jun 2009]

Uniprot Description

HPRT1: Converts guanine to guanosine monophosphate, and hypoxanthine to inosine monophosphate. Transfers the 5- phosphoribosyl group from 5-phosphoribosylpyrophosphate onto the purine. Plays a central role in the generation of purine nucleotides through the purine salvage pathway. Defects in HPRT1 are the cause of Lesch-Nyhan syndrome (LNS). LNS is characterized by complete lack of enzymatic activity that results in hyperuricemia, choreoathetosis, mental retardation, and compulsive self-mutilation. Defects in HPRT1 are the cause of gout HPRT-related (GOUT-HPRT); also known as HPRT-related gout or Kelley-Seegmiller syndrome. Gout is characterized by partial enzyme activity and hyperuricemia. Belongs to the purine/pyrimidine phosphoribosyltransferase family.

Protein type: Xenobiotic Metabolism - drug metabolism - other enzymes; Nucleotide Metabolism - purine; EC 2.4.2.8; Transferase; Cell development/differentiation

Chromosomal Location of Human Ortholog: Xq26.1

Cellular Component: cytoplasm; cytosol

Molecular Function: protein binding; protein homodimerization activity; magnesium ion binding; nucleotide binding; hypoxanthine phosphoribosyltransferase activity

Biological Process: hypoxanthine metabolic process; IMP salvage; cytolysis; adenine salvage; locomotory behavior; dopamine metabolic process; purine salvage; protein homotetramerization; cerebral cortex neuron differentiation; IMP metabolic process; hypoxanthine salvage; central nervous system neuron development; grooming behavior; lymphocyte proliferation; striatum development; nucleobase, nucleoside and nucleotide metabolic process; GMP salvage; dendrite morphogenesis; response to amphetamine; purine nucleotide biosynthetic process; purine base metabolic process; GMP catabolic process; positive regulation of dopamine metabolic process; purine ribonucleoside salvage; guanine salvage

Disease: Kelley-seegmiller Syndrome; Lesch-nyhan Syndrome