Western Blot (WB)
(MBS421036 staining (0.5µg/ml) of Jurkat lysate (RIPA buffer, 35µg total protein per lane). Primary incubated for 1 hour. Detected by chemiluminescence.)
Goat HPK1/MAP4K1 Polyclonal Antibody | anti-MAP4K1 antibody
Goat anti-HPK1/MAP4K1 Antibody
Gene Names
MAP4K1; HPK1
Reactivity
Tested: Human; Expected from sequence similarity: Human, Mouse, Rat, Dog, Pig, Cow
Applications
ELISA, Western Blot, Immunohistochemistry
Purity
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Synonyms
HPK1/MAP4K1; Polyclonal Antibody; Goat anti-HPK1/MAP4K1 Antibody; MAP4K1; mitogen-activated protein kinase kinase kinase kinase 1; HPK1; hematopoietic progenitor kinase 1; MAP4K1 antibody; mitogen-activated protein kinase kinase kinase kinase 1 antibody; HPK1 antibody; hematopoietic progenitor kinase 1 antibody; HPK1 / MAP4K1; anti-MAP4K1 antibody
Host
Goat
Reactivity
Tested: Human; Expected from sequence similarity: Human, Mouse, Rat, Dog, Pig, Cow
Clonality
Polyclonal
Purity/Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Form/Format
Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Concentration
100ug specific antibody in 200ul (varies by lot)
Sequence
DVVDPDIFNRDPR
Sequence Length
821
Applicable Applications for anti-MAP4K1 antibody
Peptide ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC)
Application Notes
Peptide ELISA: Antibody detection limit dilution 1: 32000.
Western Blot: Approx 95-100kDa band observed in Jurkat (predicted MW of 96kDa according to NP_009112). Recommended for use at 0.5-2ug/ml.
IHC: Paraffin embedded Human Thymus. Recommended concentration: 2.5 ug/ml
Western Blot: Approx 95-100kDa band observed in Jurkat (predicted MW of 96kDa according to NP_009112). Recommended for use at 0.5-2ug/ml.
IHC: Paraffin embedded Human Thymus. Recommended concentration: 2.5 ug/ml
Immunogen
Peptide with sequence DVVDPDIFNRDPR-C, from the N Terminus of the protein sequence according to NP_001036065.1; NP_009112.1.
Note
This antibody is expected to recognise isoform 1 (NP_001036065.1) and isoform 2 (NP_009112.1).
Preparation and Storage
Aliquot and store at -20°C. Minimize freezing and thawing.
Western Blot (WB)
(MBS421036 staining (0.5µg/ml) of Jurkat lysate (RIPA buffer, 35µg total protein per lane). Primary incubated for 1 hour. Detected by chemiluminescence.)
Western Blot (WB)
(MBS421036 staining (0.5µg/ml) of Jurkat lysate (RIPA buffer, 35µg total protein per lane). Primary incubated for 1 hour. Detected by chemiluminescence.)
Immunohistochemistry
(MBS421036 (2.5µg/ml) staining of paraffin embedded Human Thymus. Steamed antigen retrieval with citrate bufferpH 6, AP-staining.)
Immunohistochemistry
(MBS421036 (2.5µg/ml) staining of paraffin embedded Human Thymus. Steamed antigen retrieval with citrate bufferpH 6, AP-staining.)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
NCBI Official Full Name
mitogen-activated protein kinase kinase kinase kinase 1 isoform 1
NCBI Official Synonym Full Names
mitogen-activated protein kinase kinase kinase kinase 1
NCBI Official Symbol
MAP4K1
NCBI Official Synonym Symbols
HPK1
NCBI Protein Information
mitogen-activated protein kinase kinase kinase kinase 1
UniProt Protein Name
Mitogen-activated protein kinase kinase kinase kinase 1
Protein Family
UniProt Gene Name
MAP4K1
UniProt Synonym Gene Names
HPK1; MEK kinase kinase 1; MEKKK 1
UniProt Entry Name
M4K1_HUMAN
Uniprot Description
HPK1: a protein kinase of the STE20 family. Expressed primarily in hematopoietic organs, including bone marrow, spleen and thymus. Also expressed at very low levels in lung, kidney, mammary glands and small intestine. May play a role in hematopoietic lineage decisions and growth regulation. Positively regulated by PGE2 and a negative regulator of PGE2-induced FOS gene transcription.
Protein type: Kinase, protein; Protein kinase, STE; Protein kinase, Ser/Thr (non-receptor); EC 2.7.11.1; STE group; STE20 family; KHS subfamily
Chromosomal Location of Human Ortholog: 19q13.1-q13.4
Cellular Component: cytoplasm; membrane
Molecular Function: ATP binding; MAP kinase kinase kinase kinase activity; protein binding; protein kinase activity; protein serine/threonine kinase activity
Biological Process: activation of JNK activity; activation of MAPKKK activity; cell proliferation; peptidyl-serine phosphorylation; protein amino acid autophosphorylation; protein amino acid phosphorylation; response to stress
Research Articles on MAP4K1
Similar Products
Product Notes
The MAP4K1 map4k1 (Catalog #AAA421036) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The Goat anti-HPK1/MAP4K1 Antibody reacts with Tested: Human; Expected from sequence similarity: Human, Mouse, Rat, Dog, Pig, Cow and may cross-react with other species as described in the data sheet. AAA Biotech's HPK1/MAP4K1 can be used in a range of immunoassay formats including, but not limited to, Peptide ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC). Peptide ELISA: Antibody detection limit dilution 1: 32000. Western Blot: Approx 95-100kDa band observed in Jurkat (predicted MW of 96kDa according to NP_009112). Recommended for use at 0.5-2ug/ml. IHC: Paraffin embedded Human Thymus. Recommended concentration: 2.5 ug/ml. Researchers should empirically determine the suitability of the MAP4K1 map4k1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: DVVDPDIFNR DPR. It is sometimes possible for the material contained within the vial of "HPK1/MAP4K1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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