Rabbit anti-Human, Mouse HP Polyclonal Antibody | anti-HP antibody

HP, ID (HP, Haptoglobin, Zonulin, Haptoglobin alpha chain, Haptoglobin beta chain) (PE)

Cat. Num. AAA6308209

• Gene Names: HP; BP; HPA1S; HP2ALPHA2
• Reactivity: Human, Mouse
• Applications: Immunohistochemistry, Western Blot, Immunofluorescence, Flow Cytometry, Functional Assay
• Purity: Purified by Protein A and Peptide Affinity Chromatography.
• Synonyms: HP; Polyclonal Antibody; ID (HP; Haptoglobin; Zonulin; Haptoglobin alpha chain; Haptoglobin beta chain) (PE); Haptoglobin beta chain; anti-HP antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human HP. Species crossreactivity: mouse
• Purity/Purification: Purified by Protein A and Peptide Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).

• Applicable Applications for anti-HP antibody: Immunohistochemistry (IHC) Paraffin, Western Blot (WB), Immunofluorescence (IF), Flow Cytometry (FC/FACS), FLISA
• Application Notes: Applications are based on unconjugated antibody.
• Immunogen: KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 296-322 in the central region of human HP.
• Conjugate: PE
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western Blot analysis of HP,(arrow) in 293 cell lysates (2ug/lane) using 036741 either nontransfected (Lane 1) or transiently transfected (Lane 2) with the HP gene.)

Western Blot (WB)

(Western Blot analysis in MDA-MB453, CEM, NCI-H460, HepG2, K562 cell line lysates (35ug/lane) using 036741. This demonstrates that 036741 detected the HP protein (arrow).)

Immunohistochemistry (IHC)

(Immunohistochemistry analysis in formalin fixed and paraffin embedded human hepatocarcinoma using 036741 followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of 036741 for immunohistochemistry.)

Flow Cytometry (FC/FACS)

(Flow Cytometric analysis of MDA-MB435 cells using 036741 (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Immunofluorescence (IF)

(Confocal immunofluorescent analysis of MDA-MB435 cells using 036741 followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).)

Product Categories/Family for anti-HP antibody

Antibodies; Serum Proteins

References

1. Bailey, S.D., et al. Diabetes Care 33(10):2250-2253(2010). 2. Ucisik-Akkaya, E., et al. Mol. Hum. Reprod. 16(10):770-777(2010). 3. Ruano, G., et al. Pharmacogenomics 11(7):959-971(2010). 4. Savy, M., et al. PLoS ONE 5 (6), E11075 (2010). 5. Kasvosve, I., et al. Adv Clin Chem 50, 23-46 (2010).

NCBI and Uniprot Product Information

• NCBI GI #: 4826762
• NCBI GeneID: 3240
• NCBI Accession #: NP_005134.1
• NCBI GenBank Nucleotide #: NM_005143.3
• UniProt Accession #: P00738; P00737; Q0VAC4; Q0VAC5; Q2PP15; Q3B7J0; Q6LBY9; Q9UC67; B0AZL5
• Molecular Weight: 38,452 Da
• NCBI Official Full Name: haptoglobin isoform 1 preproprotein
• NCBI Official Synonym Full Names: haptoglobin
• NCBI Official Symbol: HP
• NCBI Official Synonym Symbols: BP; HPA1S; HP2ALPHA2
• NCBI Protein Information: haptoglobin; zonulin; binding peptide; haptoglobin alpha(1S)-beta; haptoglobin alpha(2FS)-beta; haptoglobin, beta polypeptide; haptoglobin, alpha polypeptide
• UniProt Protein Name: Haptoglobin
• Protein Family: HPr-like protein
• UniProt Gene Name: HP
• UniProt Entry Name: HPT_HUMAN

NCBI Description

This gene encodes a preproprotein, which is processed to yield both alpha and beta chains, which subsequently combine as a tetramer to produce haptoglobin. Haptoglobin functions to bind free plasma hemoglobin, which allows degradative enzymes to gain access to the hemoglobin, while at the same time preventing loss of iron through the kidneys and protecting the kidneys from damage by hemoglobin. Mutations in this gene and/or its regulatory regions cause ahaptoglobinemia or hypohaptoglobinemia. This gene has also been linked to diabetic nephropathy, the incidence of coronary artery disease in type 1 diabetes, Crohn's disease, inflammatory disease behavior, primary sclerosing cholangitis, susceptibility to idiopathic Parkinson's disease, and a reduced incidence of Plasmodium falciparum malaria. The protein encoded also exhibits antimicrobial activity against bacteria. A similar duplicated gene is located next to this gene on chromosome 16. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2014]

Uniprot Description

HP: Haptoglobin combines with free plasma hemoglobin, preventing loss of iron through the kidneys and protecting the kidneys from damage by hemoglobin, while making the hemoglobin accessible to degradative enzymes. Defects in HP are the cause of anhaptoglobinemia (AHP). AHP is a condition characterized by the absence of the serum glycoprotein haptoglobin. Serum levels of haptoglobin vary among normal persons: levels are low in the neonatal period and in the elderly, differ by population, and can be influenced by environmental factors, such as infection. Secondary hypohaptoglobinemia can occur as a consequence of hemolysis, during which haptoglobin binds to free hemoglobin. Belongs to the peptidase S1 family.

Protein type: Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 16q22.2

Cellular Component: extracellular space; extracellular region

Molecular Function: antioxidant activity; protein binding; hemoglobin binding; catalytic activity

Biological Process: receptor-mediated endocytosis; response to hydrogen peroxide; immune system process; metabolic process; defense response to bacterium; negative regulation of oxidoreductase activity; acute-phase response; defense response

Disease: Anhaptoglobinemia