Rabbit anti-Human, Mouse Hoxd10 Polyclonal Antibody | anti-Hoxd10 antibody

Hoxd10, ID (Hoxd10, Hox-4.5, Hoxd-10, Homeobox protein Hox-D10, Homeobox protein Hox-4.5, Homeobox protein Hox-5.3) (AP)

Cat. Num. AAA6308089

• Gene Names: Hoxd10; Hox-4.5; Hox-5.3; AI385591; AI874987
• Reactivity: Human, Mouse
• Applications: Immunohistochemistry, ELISA, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: Hoxd10; Polyclonal Antibody; ID (Hoxd10; Hox-4.5; Hoxd-10; Homeobox protein Hox-D10; Homeobox protein Hox-4.5; Homeobox protein Hox-5.3) (AP); Homeobox protein Hox-5.3; anti-Hoxd10 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Alkaline Phosphatase (AP).

• Applicable Applications for anti-Hoxd10 antibody: Immunohistochemistry (IHC), ELISA (EIA), Western Blot (WB)
• Application Notes: IHC: 1:10-50; ELISA: 1:1,000; WB: 1:100-500; Applications are based on unconjugated antibody.
• Immunogen: Mouse Hoxd10 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 200-230 amino acids from the Central region of Mouse Hoxd10.
• Conjugate: AP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Mouse Hoxd10 Antibody (Center) western blot analysis in NCI-H292 cell line lysates (35ug/lane).This demonstrates the Hoxd10 antibody detected the Hoxd10 protein (arrow).)

Immunohistochemistry (IHC)

(Mouse Hoxd10 Antibody (Center) (AP14309c)immunohistochemistry analysis in formalin fixed and paraffin embedded mouse uterus tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of Mouse Hoxd10 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.)

Related Product Information for anti-Hoxd10 antibody

Sequence-specific transcription factor which is part of a developmental regulatory system that provides cells with specific positional identities on the anterior-posterior axis.

Product Categories/Family for anti-Hoxd10 antibody

Antibodies; Transcription Factors; Zinc; Ring; Homeobox

References

Mugford, J.W., et al. Dev. Biol. 333(2):312-323(2009). Cocquempot, O., et al. Genetics 183(1):23-30(2009). Misra, M., et al. Dev. Biol. 330(1):54-72(2009). Hibiya, K., et al. Dev. Biol. 329(2):176-190(2009). Tschopp, P., et al. PLoS Genet. 5 (3), E1000398 (2009).

NCBI and Uniprot Product Information

• NCBI GI #: 31560501
• NCBI GeneID: 15430
• NCBI Accession #: NP_038582.2
• NCBI GenBank Nucleotide #: NM_013554.5
• UniProt Accession #: P28359; Q91WU9
• Molecular Weight: 38,328 Da
• NCBI Official Full Name: homeobox protein Hox-D10
• NCBI Official Synonym Full Names: homeobox D10
• NCBI Official Symbol: Hoxd10
• NCBI Official Synonym Symbols: Hox-4.5; Hox-5.3; AI385591; AI874987
• NCBI Protein Information: homeobox protein Hox-D10
• UniProt Protein Name: Homeobox protein Hox-D10
• Protein Family: Homeobox protein
• UniProt Gene Name: Hoxd10
• UniProt Synonym Gene Names: Hox-4.5; Hoxd-10
• UniProt Entry Name: HXD10_MOUSE

Uniprot Description

HOXD10: Sequence-specific transcription factor which is part of a developmental regulatory system that provides cells with specific positional identities on the anterior-posterior axis. Defects in HOXD10 are a cause of congenital vertical talus (CVT); also known as rocker-bottom foot deformity or congenital convex pes valgus. CVT is a dislocation of the talonavicular joint, with rigid dorsal dislocation of the navicular over the neck of the talus. This condition is usually associated with multiple other congenital deformities and only rarely is an isolated deformity. Belongs to the Abd-B homeobox family.

Protein type: DNA-binding

Cellular Component: nucleus

Molecular Function: protein binding; DNA binding; sequence-specific DNA binding; chromatin binding; transcription factor activity

Biological Process: hindlimb morphogenesis; skeletal muscle development; transcription, DNA-dependent; adult locomotory behavior; neuromuscular process; multicellular organismal development; spinal cord motor neuron cell fate specification; embryonic skeletal morphogenesis; forelimb morphogenesis; anterior/posterior pattern formation; peripheral nervous system neuron development; regulation of transcription, DNA-dependent; regulation of gene expression; single fertilization; positive regulation of transcription from RNA polymerase II promoter; skeletal development; proximal/distal pattern formation; embryonic limb morphogenesis