Rabbit anti-Human, Mouse HOXA1 Polyclonal Antibody | anti-HOXA1 antibody

HOXA1, NT (HOXA1, HOX1F, Homeobox protein Hox-A1, Homeobox protein Hox-1F) (Biotin)

Cat. Num. AAA6307640

• Gene Names: HOXA1; BSAS; HOX1; HOX1F
• Reactivity: Human, Mouse
• Applications: Western Blot, ELISA
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: HOXA1; Polyclonal Antibody; NT (HOXA1; HOX1F; Homeobox protein Hox-A1; Homeobox protein Hox-1F) (Biotin); Homeobox protein Hox-1F; anti-HOXA1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin.

• Applicable Applications for anti-HOXA1 antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:100-500; ELISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: HOXA1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 8-35 amino acids from the N-terminal region of human HOXA1.
• Conjugate: Biotin
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(HOXA1 Antibody (N-term) western blot analysis in mouse liver tissue lysates (35ug/lane).This demonstrates the HOXA1 antibody detected the HOXA1 protein (arrow).)

Related Product Information for anti-HOXA1 antibody

In vertebrates, the genes encoding the class of transcription factors called homeobox genes are found in clusters named A, B, C, and D on four separate chromosomes. Expression of these proteins is spatially and temporally regulated during embryonic development. This gene is part of the A cluster on chromosome 7 and encodes a DNA-binding transcription factor which may regulate gene expression, morphogenesis, and differentiation. The encoded protein may be involved in the placement of hindbrain segments in the proper location along the anterior-posterior axis during development. Two transcript variants encoding two different isoforms have been found for this gene, with only one of the isoforms containing the homeodomain region.

Product Categories/Family for anti-HOXA1 antibody

Antibodies; Transcription Factors; Zinc; Ring; Homeobox

References

Rankin, J.K., et al. J AAPOS 14(1):78-80(2010). Muscarella, L.A., et al. Mol Autism 1 (1), 9 (2010): Canu, E., et al. J Neuroimaging 19(4):353-358(2009). Gratacos, M., et al. Am. J. Med. Genet. B Neuropsychiatr. Genet. 150B (6), 808-816 (2009): Chakrabarti, B., et al. Autism Res 2(3):157-177(2009).

NCBI and Uniprot Product Information

• NCBI GI #: 5031761
• NCBI GeneID: 3198
• NCBI Accession #: NP_005513.1
• NCBI GenBank Nucleotide #: NM_005522.4
• UniProt Accession #: P49639; O43363; A4D184; B2R8U7
• Molecular Weight: 36,641 Da
• NCBI Official Full Name: homeobox protein Hox-A1 isoform a
• NCBI Official Synonym Full Names: homeobox A1
• NCBI Official Symbol: HOXA1
• NCBI Official Synonym Symbols: BSAS; HOX1; HOX1F
• NCBI Protein Information: homeobox protein Hox-A1; homeobox 1F; homeo box A1; lab-like protein; Hox 1.6-like protein; homeobox protein Hox-1F; HOX A1 homeodomain protein
• UniProt Protein Name: Homeobox protein Hox-A1
• Protein Family: Homeobox protein
• UniProt Gene Name: HOXA1
• UniProt Synonym Gene Names: HOX1F
• UniProt Entry Name: HXA1_HUMAN

NCBI Description

In vertebrates, the genes encoding the class of transcription factors called homeobox genes are found in clusters named A, B, C, and D on four separate chromosomes. Expression of these proteins is spatially and temporally regulated during embryonic development. This gene is part of the A cluster on chromosome 7 and encodes a DNA-binding transcription factor which may regulate gene expression, morphogenesis, and differentiation. The encoded protein may be involved in the placement of hindbrain segments in the proper location along the anterior-posterior axis during development. Two transcript variants encoding two different isoforms have been found for this gene, with only one of the isoforms containing the homeodomain region. [provided by RefSeq, Jul 2008]

Uniprot Description

HOXA1: Sequence-specific transcription factor which is part of a developmental regulatory system that provides cells with specific positional identities on the anterior-posterior axis. Acts on the anterior body structures. Seems to act in the maintenance and/or generation of hindbrain segments. Defects in HOXA1 are the cause of Athabaskan brainstem dysgenesis syndrome (ABDS); also known as Narvajo brainstem syndrome. This syndrome is characterized by horizontal gaze palsy, sensorineural deafness, central hypoventilation, and developmental delay. Some patients had swallowing dysfunction, vocal cord paralysis, facial paresis, seizures, and cardiac outflow tract anomalies. Defects in HOXA1 are the cause of Bosley-Salih-Alorainy syndrome (BSAS). Affected individuals show horizontal gaze abnormalities, deafness, facial weakness, vascular malformations of the internal carotid arteries and cardiac outflow trac. Some patients manifest mental retardation and autism spectrum disorder. In contrast to individuals with ABSD, central hypoventilation is not observed in individuals with BSAS. Belongs to the Antp homeobox family. Labial subfamily. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: DNA-binding; Oncoprotein; Transcription factor

Chromosomal Location of Human Ortholog: 7p15.3

Cellular Component: nucleus

Molecular Function: protein binding; sequence-specific DNA binding

Biological Process: facial nerve structural organization; anatomical structure morphogenesis; central nervous system neuron differentiation; facial nucleus development; transcription, DNA-dependent; multicellular organismal development; neuromuscular process; outer ear morphogenesis; motor axon guidance; rhombomere 4 development; anterior/posterior pattern formation; sensory perception of sound; optokinetic behavior; artery morphogenesis; embryonic neurocranium morphogenesis; positive regulation of transcription from RNA polymerase II promoter; cognition; rhombomere 5 development; inner ear development; rhombomere 3 development; regulation of behavior; abducens nerve formation

Disease: Athabaskan Brainstem Dysgenesis Syndrome