Rabbit HOMER1 Polyclonal Antibody | anti-HOMER1 antibody

HOMER1 Antibody (N-term)

Cat. Num. AAA9202017

• Gene Names: HOMER1; HOMER; SYN47; Ves-1; HOMER1A; HOMER1B; HOMER1C
• Reactivity: Human (Predicted Reactivity: Bovine, Mouse, Rat)
• Applications: Western Blot, ELISA, Immunohistochemistry, Flow Cytometry, Functional Assay
• Purity: Purified Rabbit Polyclonal Antibody (Pab)
• Synonyms: HOMER1; Polyclonal Antibody; HOMER1 Antibody (N-term); Homer protein homolog 1; Homer-1; SYN47; anti-HOMER1 antibody

• Host: Rabbit
• Reactivity: Human (Predicted Reactivity: Bovine, Mouse, Rat)
• Clonality: Polyclonal
• Isotype: Rabbit Ig
• Specificity: This HOMER1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 96-122 amino acids from the N-terminal region of human HOMER1.
• Purity/Purification: Purified Rabbit Polyclonal Antibody (Pab)
• Form/Format: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
• Concentration: Vial Concentration: 2 (varies by lot)
• Sequence Positions: 96-122
• Sequence Length: 224

• Applicable Applications for anti-HOMER1 antibody: Western Blot (WB), ELISA (EIA), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS)
• Application Notes: WB~~1:1000
• Antigen Type: Synthetic Peptide
• Antigen Source: HUMAN
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

Western Blot (WB)

(Western blot analysis of HOMER1 antibody (N-term) in HepG2 cell line lysates (35ug/lane). HOMER1 (arrow) was detected using the purified Pab.)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human hepatocarcinoma with HOMER1 Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)

Flow Cytometry (FC/FACS)

(HOMER1 Antibody (N-term) flow cytometry analysis of HepG2 cells (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Related Product Information for anti-HOMER1 antibody

HOMER1 is a member of the homer family of dendritic proteins. Members of this family regulate group 1 metabotrophic glutamate receptor function.

Product Categories/Family for anti-HOMER1 antibody

Neuroscience

References

Sanna,S., Jackson,A.U. Nat. Genet. 40 (2), 198-203 (2008)
Dahl,J.P., Kampman,K.M. Psychiatr. Genet. 15 (4), 277-283 (2005)
Tu,J.C., Xiao,B. Neuron 23 (3), 583-592 (1999)

NCBI and Uniprot Product Information

• NCBI GI #: 459215038
• NCBI GeneID: 9456
• NCBI Accession #: NP_001264006.1; NP_001264007.1; NP_004263.1
• NCBI GenBank Nucleotide #: NM_001277077.1
• UniProt Accession #: Q86YM7; O96003; Q86YM5; B2R688
• Molecular Weight: 40277
• NCBI Official Full Name: homer protein homolog 1 isoform 2
• NCBI Official Synonym Full Names: homer scaffolding protein 1
• NCBI Official Symbol: HOMER1
• NCBI Official Synonym Symbols: HOMER; SYN47; Ves-1; HOMER1A; HOMER1B; HOMER1C
• NCBI Protein Information: homer protein homolog 1
• UniProt Protein Name: Homer protein homolog 1
• Protein Family: Homer protein
• UniProt Gene Name: HOMER1
• UniProt Synonym Gene Names: SYN47; Homer-1
• UniProt Entry Name: HOME1_HUMAN

NCBI Description

This gene encodes a member of the homer family of dendritic proteins. Members of this family regulate group 1 metabotrophic glutamate receptor function. [provided by RefSeq, Jul 2008]

Uniprot Description

HOMER1: Postsynaptic density scaffolding protein. Binds and cross-links cytoplasmic regions of GRM1, GRM5, ITPR1, DNM3, RYR1, RYR2, SHANK1 and SHANK3. By physically linking GRM1 and GRM5 with ER-associated ITPR1 receptors, it aids the coupling of surface receptors to intracellular calcium release. May also couple GRM1 to PI3 kinase through its interaction with AGAP2. Isoform 1 regulates the trafficking and surface expression of GRM5. Isoform 3 acts as a natural dominant negative, in dynamic competition with constitutively expressed isoform 1 to regulate synaptic metabotropic glutamate function. Isoform 3, may be involved in the structural changes that occur at synapses during long-lasting neuronal plasticity and development. Interacts with IFT57. Interacts with OPHN1. Isoform 1 encodes a coiled-coil structure that mediates homo- and heteromultimerization. Belongs to the Homer family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Adaptor/scaffold

Chromosomal Location of Human Ortholog: 5q14.2

Cellular Component: postsynaptic membrane; costamere; cell soma; apical part of cell; axon; excitatory synapse; dendritic shaft; cell junction; Z disc

Molecular Function: identical protein binding; metabotropic glutatmate receptor binding; protein heterodimerization activity; type 5 metabotropic glutamate receptor binding; protein complex scaffold

Biological Process: circadian rhythm; skeletal muscle contraction; response to nicotine; synaptic transmission; chemical homeostasis within a tissue; positive regulation of signal transduction; behavioral response to cocaine; metabotropic glutamate receptor, phospholipase C activating pathway; skeletal muscle fiber development; response to calcium ion; positive regulation of calcium ion transport