Rabbit hns Polyclonal Antibody | anti-hns antibody

hns Antibody

Cat. Num. AAA7134611

• Gene Names: hns; B1; bglY; cur; drc; drdX; drs; ECK1232; fimG; H1; hnsA; irk; JW1225; msyA; osmZ; pilG; topS; topX; verA; virR
• Reactivity: Escherichia coli
• Applications: ELISA, Western Blot
• Purity: >95%, Protein G Purified
• Synonyms: hns; Polyclonal Antibody; hns Antibody; DNA-binding protein H-NS; Heat-stable Nucleoid-structuring protein; Histone-like protein HLP-II; Protein B1; Protein H1; bglY; cur; drdX; hnsA; msyA; osmZ; pilG; topS; anti-hns antibody

• Host: Rabbit
• Reactivity: Escherichia coli
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: >95%, Protein G Purified
• Form/Format: Liquid; Preservative: 0.03% Proclin 300; Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

• Applicable Applications for anti-hns antibody: ELISA (EIA), Western Blot (WB)
• Application Notes: WB: 1:2000-1:10000
• Immunogen: Recombinant Escherichia coli DNA-binding protein H-NS protein (39-137AA)
• Immunogen Species: Escherichia coli (strain K12)
• Conjugate: Non-conjugated
• Preparation and Storage: Upon receipt, store at -20 degree C or -80 degree C. Avoid repeated freeze.

Western Blot (WB)

(Western BlotPositive WB detected in Recombinant proteinAll lanes: hns antibody at 1:2500SecondaryGoat polyclonal to rabbit IgG at 1/50000 dilutionPredicted band size: 38 kDaObserved band size: 40 kDa)

NCBI and Uniprot Product Information

• NCBI GI #: 16129198
• NCBI GeneID: 945829
• NCBI Accession #: NP_415753.1
• NCBI GenBank Nucleotide #: NC_000913.3
• UniProt Accession #: P0ACF8; P08936; Q47267
• Molecular Weight: 19.5 kDa
• NCBI Official Full Name: global DNA-binding transcriptional dual regulator H-NS
• NCBI Official Symbol: hns
• NCBI Official Synonym Symbols: B1; bglY; cur; drc; drdX; drs; ECK1232; fimG; H1; hnsA; irk; JW1225; msyA; osmZ; pilG; topS; topX; verA; virR
• NCBI Protein Information: global DNA-binding transcriptional dual regulator H-NS
• UniProt Protein Name: DNA-binding protein H-NS
• Protein Family: DNA-binding protein
• UniProt Gene Name: hns

NCBI Description

HN-S forms heteromeric complexes with Hha and Cnu, which modulates HN-S function, expression and stability. [More information is available at EcoGene: EG10457]. Heat-stable nucleoid-structuring protein (H-NS) is a nucleoid-associated protein that acts as a transcriptional repressor and may serve to generally suppress horizontally acquired genes. [More information is available at EcoCyc: EG10457].

Uniprot Description

A DNA-binding protein implicated in transcriptional repression (silencing) (PubMed:333393, PubMed:2128918, PubMed:8890170, PubMed:8913298, PubMed:9398522, PubMed:16963779, PubMed:17046956, PubMed:23543115). Also involved in bacterial chromosome organization and compaction (PubMed:6379600, PubMed:10982869, PubMed:21903814). H-NS binds tightly to AT-rich dsDNA and inhibits transcription (PubMed:2512122, PubMed:16963779, PubMed:17435766, PubMed:17881364, PubMed:23543115). Binds upstream and downstream of initiating RNA polymerase, trapping it in a loop and preventing transcription (PubMed:11714691). Binds to hundreds of sites, approximately half its binding sites are in non-coding DNA, which only accounts for about 10% of the genome (PubMed:16963779, PubMed:17046956, PubMed:23543115). Many of these loci were horizontally transferred (HTG); this offers the selective advantage of silencing foreign DNA while keeping it in the genome in case of need (PubMed:17046956, PubMed:17881364, PubMed:26789284). Suppresses transcription at many intragenic sites as well as transcription of spurious, non-coding RNAs genome-wide (PubMed:24449106). Repression of HTG by H-NS is thought to allow their DNA to evolve faster than non-H-NS-bound regions, and facilitates integration of HTG into transcriptional regulatory networks (PubMed:26789284). A subset of H-NS/StpA-regulated genes also require Hha (and/or Cnu, ydgT) for repression; Hha and Cnu increase the number of genes DNA bound by H-NS/StpA and may also modulate the oligomerization of the H-NS/StpA-complex (PubMed:23543115). The protein forms 2 clusters in the nucleoid which gather hns-bound loci together, bridging non-contiguous DNA, and causes DNA substantial condensation (PubMed:21903814). Binds DNA better at low temperatures than at 37 degrees Celsius; AT-rich sites nucleate H-NS binding, further DNA-binding is cooperative and this cooperativity decreases with rising temperature (PubMed:17435766, PubMed:17881364). Transcriptional repression can be inhibited by dominant-negative mutants of StpA or itself (PubMed:8755860). May effect transcriptional elongation (PubMed:25638302). Can increase translational efficiency of mRNA with suboptimal Shine-Dalgarno sequences (PubMed:20595230). Plays a role in the thermal control of pili and adhesive curli fimbriae production, by inducing transcription of csgD (PubMed:17010156). Plays a role in flagellar function (PubMed:11031114). Represses the CRISPR-cas promoters, permits only weak transcription of the crRNA precursor; its repression is antagonized by LeuO (PubMed:20132443, PubMed:20659289). Binds preferentially to the upstream region of its own gene recognizing two segments of DNA on both sides of a bend centered around -150 (PubMed:7934818). Overexpression suppresses secY24, a temperature-sensitive mutation (PubMed:1537791). Has also been reported to activate transcription of some genes (PubMed:4566454, PubMed:338303, PubMed:2128918).