Rabbit anti-Human HNRNPAB Polyclonal Antibody | anti-HNRNPAB antibody

Rabbit Polyclonal to Human HNRNPAB

Cat. Num. AAA248150

• Gene Names: HNRNPAB; ABBP1; HNRPAB
• Reactivity: Human
• Applications: Immunohistochemistry, Immunofluorescence, Western Blot
• Purity: Immunoaffinity Purified
• Synonyms: HNRNPAB; Polyclonal Antibody; Rabbit Polyclonal to Human HNRNPAB; Anti-HNRNPAB Antibody (aa8-38) IHC-plus; ABBP-1; ABBP1; APOBEC1-binding protein 1; HnRNP type A/B protein; HnRNP A/B; HNRPAB; Apobec-1 binding protein 1; Human HNRNPAB; anti-HNRNPAB antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Specificity: Human HNRNPAB
• Purity/Purification: Immunoaffinity Purified
• Form/Format: PBS, 0.09% sodium azide
• Sequence Length: 332

• Applicable Applications for anti-HNRNPAB antibody: Immunohistochemistry (IHC - Paraffin), Immunofluorescence (IF), Western Blot (WB)
• Application Notes: IF (1:10 - 1:50), IHC-P (1:50 - 1:100), WB (1:1000)
• Target Species: Human
• Immunogen Description: KLH-conjugated synthetic peptide from N-terminal region of human HNRPAB.
• Immunogen Type: Synthetic peptide
• Immunogen: HNRNPAB antibody was raised against kLH-conjugated synthetic peptide from N-terminal region of human HNRPAB.
• Antigen Modification: aa8-38
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C.

Immunohistochemistry (IHC)

(Anti-HNRNPAB antibody IHC staining of human uterus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.)

Immunohistochemistry (IHC)

(Anti-HNRNPAB antibody IHC staining of human tonsil. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.)

Immunofluorescence (IF)

(Fluorescent confocal image of MCF-7 cell stained with HNRPAB Antibody (N-term).MCF-7 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with HNRPAB primary antibody (1:25, 1 h at 37°C). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37°C).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37°C). Nuclei were counterstained with DAPI (blue) (10 ug/ml, 10 min). HNRPAB immunoreactivity is localized to Nucleus significantly.)

Western Blot (WB)

(HNRPAB Antibody (N-term) Western blot of A375, T47D cell line lysates (35 ug/lane). This demonstrates the HNRPAB antibody detected the HNRPAB protein (arrow).)

NCBI and Uniprot Product Information

• NCBI GI #: 55956919
• NCBI GeneID: 3182
• NCBI Accession #: NP_112556.2
• NCBI GenBank Nucleotide #: NM_031266.2
• Molecular Weight: 30,701 Da
• NCBI Official Full Name: heterogeneous nuclear ribonucleoprotein A/B isoform a
• NCBI Official Synonym Full Names: heterogeneous nuclear ribonucleoprotein A/B
• NCBI Official Symbol: HNRNPAB
• NCBI Official Synonym Symbols: ABBP1; HNRPAB
• NCBI Protein Information: heterogeneous nuclear ribonucleoprotein A/B; ABBP-1; hnRNP A/B; hnRNP type A/B protein; APOBEC1-binding protein 1; apobec-1 binding protein 1; apolipoprotein B mRNA editing enzyme, catalytic polypeptide 1-binding protein 1
• UniProt Protein Name: Heterogeneous nuclear ribonucleoprotein A/B
• Protein Family: Heterogeneous nuclear ribonucleoprotein
• UniProt Gene Name: HNRNPAB
• UniProt Synonym Gene Names: ABBP1; HNRPAB; hnRNP A/B; ABBP-1
• UniProt Entry Name: ROAA_HUMAN

NCBI Description

This gene belongs to the subfamily of ubiquitously expressed heterogeneous nuclear ribonucleoproteins (hnRNPs). The hnRNPs are produced by RNA polymerase II and are components of the heterogeneous nuclear RNA (hnRNA) complexes. They are associated with pre-mRNAs in the nucleus and appear to influence pre-mRNA processing and other aspects of mRNA metabolism and transport. While all of the hnRNPs are present in the nucleus, some seem to shuttle between the nucleus and the cytoplasm. The hnRNP proteins have distinct nucleic acid binding properties. The protein encoded by this gene, which binds to one of the components of the multiprotein editosome complex, has two repeats of quasi-RRM (RNA recognition motif) domains that bind to RNAs. Two alternatively spliced transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

hnRNP A/B: a single-stranded RNA binding protein with two RNA-recognition (RRM) domains. Has a high affinity for G- rich and U-rich regions of hnRNA. Also binds to APOB mRNA transcripts around the RNA editing site. Interacts with APOBEC1. Observed in a mRNP granule complex, at least composed of ACTB, ACTN4, DHX9, ERG, HNRNPA1, HNRNPA2B1, HNRNPAB, HNRNPD, HNRNPL, HNRNPR, HNRNPU, HSPA1, HSPA8, IGF2BP1, ILF2, ILF3, NCBP1, NCL, PABPC1, PABPC4, PABPN1, RPLP0, RPS3, RPS3A, RPS4X, RPS8, RPS9, SYNCRIP, TROVE2, YBX1 and untranslated mRNAs. A member of the RNA recognition motif (RRM) family. Four isoforms of the human protein are produced by alternative splicing.

Protein type: RNA-binding

Chromosomal Location of Human Ortholog: 5q35.3

Cellular Component: nucleoplasm; cytoplasm; ribonucleoprotein complex; nucleus

Molecular Function: mRNA binding; RNA binding; sequence-specific DNA binding; nucleotide binding; transcription factor activity

Biological Process: positive regulation of transcription, DNA-dependent; epithelial to mesenchymal transition; negative regulation of transcription from RNA polymerase II promoter