Rabbit anti-Human HNF4A Polyclonal Antibody | anti-HNF4A antibody

HNF4A, ID (Hepatocyte Nuclear Factor 4-alpha, HNF-4-alpha, Transcription Factor HNF-4, Nuclear Receptor Subfamily 2 Group A Member 1, Transcription Factor 14, HNF4, NR2A1, TCF14) (MaxLight 405)

Cat. Num. AAA6480018

• Gene Names: HNF4A; TCF; HNF4; MODY; FRTS4; MODY1; NR2A1; TCF14; HNF4a7; HNF4a8; HNF4a9; NR2A21; HNF4alpha
• Reactivity: Human
• Applications: Western Blot
• Purity: Purified by Ammonium Sulfate Precipitation.
• Synonyms: HNF4A; Polyclonal Antibody; ID (Hepatocyte Nuclear Factor 4-alpha; HNF-4-alpha; Transcription Factor HNF-4; Nuclear Receptor Subfamily 2 Group A Member 1; Transcription Factor 14; HNF4; NR2A1; TCF14) (MaxLight 405); anti-HNF4A antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human HNF4A.
• Purity/Purification: Purified by Ammonium Sulfate Precipitation.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight405.

• Applicable Applications for anti-HNF4A antibody: Western Blot (WB), FLISA
• Application Notes: WB: 1:50-1:100; FLISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: Synthetic peptide selected from the center region of human HNF4A (KLH).
• Conjugate: MaxLight405
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight405 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(HNF4A Antibody (Center) western blot analysis in 293,HepG2 cell line and rat liver tissue lysates (35ug/lane).This demonstrates the HNF4A antibody detected the HNF4A protein (arrow).)

Immunohistochemistry (IHC)

(HNF4A Antibody (Center) (AP2778c)immunohistochemistry analysis in formalin fixed and paraffin embedded human kidney tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of HNF4A Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.)

Immunofluorescence (IF)

(Confocal immunofluorescent analysis of HNF4A Antibody (Center) with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red).DAPI was used to stain the cell nuclear (blue).)

Flow Cytometry (FC/FACS)

(HNF4A Antibody (Center) flow cytometric analysis of NCI-H460 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Product Categories/Family for anti-HNF4A antibody

Antibodies; Transcription Factors

References

1. Kritis A.A., Argyrokastritis A.Gene 173:275-280(1996). 2. Yamagata K., Furuta H., Oda N.Nature 384:458-460(1996). 3. Furuta H., Iwasaki N., Oda N.Diabetes 46:1652-1657(1997). 4. Moeller A.M., Urhammer S.A.Diabetologia 40:980-983(1997).

NCBI and Uniprot Product Information

• NCBI GI #: 31077205
• NCBI GeneID: 3172
• NCBI Accession #: NP_000448
• NCBI GenBank Nucleotide #: NM_000457
• UniProt Accession #: P41235; O00659; O00723; Q14540; Q5QPB8; Q6B4V5; Q6B4V6; Q6B4V7; Q92653; Q92654; A5JW41; B2RPP8
• Molecular Weight: 43,988 Da
• NCBI Official Full Name: hepatocyte nuclear factor 4-alpha isoform HNF4alpha2
• NCBI Official Synonym Full Names: hepatocyte nuclear factor 4, alpha
• NCBI Official Symbol: HNF4A
• NCBI Official Synonym Symbols: TCF; HNF4; MODY; FRTS4; MODY1; NR2A1; TCF14; HNF4a7; HNF4a8; HNF4a9; NR2A21; HNF4alpha
• NCBI Protein Information: hepatocyte nuclear factor 4-alpha; HNF4alpha10/11/12; TCF-14; hepatic nuclear factor 4 alpha; nuclear receptor subfamily 2 group A member 1; transcription factor 14; transcription factor HNF-4
• UniProt Protein Name: Hepatocyte nuclear factor 4-alpha
• Protein Family: Hepatocyte nuclear factor
• UniProt Gene Name: HNF4A
• UniProt Synonym Gene Names: HNF4; NR2A1; TCF14; HNF-4-alpha; TCF-14
• UniProt Entry Name: HNF4A_HUMAN

Uniprot Description

HNF4 alpha: a nuclear transcription factor which binds DNA as a homodimer. Controls the expression of several genes, including hepatocyte nuclear factor 1 alpha, a transcription factor which regulates the expression of several hepatic gene, alpha 1-antitrypsin, apolipoprotein CIII, and transthyretin genes. May be essential for development of the liver, kidney and intestine. Mutations have been associated with monogenic autosomal dominant non-insulin-dependent diabetes mellitus type I. Four alternatively spliced isoforms have been described.

Protein type: Nuclear receptor; Transcription factor; DNA-binding

Chromosomal Location of Human Ortholog: 20q13.12

Cellular Component: nucleoplasm; cytoplasm; nucleus

Molecular Function: RNA polymerase II transcription factor activity, enhancer binding; protein binding; ligand-dependent nuclear receptor activity; protein homodimerization activity; DNA binding; zinc ion binding; steroid hormone receptor activity; fatty acid binding; transcription factor activity; receptor binding

Biological Process: transcription initiation from RNA polymerase II promoter; intracellular receptor-mediated signaling pathway; regulation of lipid metabolic process; positive regulation of transcription, DNA-dependent; lipid homeostasis; glucose homeostasis; endocrine pancreas development; regulation of transcription from RNA polymerase II promoter; negative regulation of cell proliferation; regulation of gastrulation; xenobiotic metabolic process; response to glucose stimulus; ornithine metabolic process; steroid hormone mediated signaling; positive regulation of transcription from RNA polymerase II promoter; gene expression; lipid metabolic process; negative regulation of cell growth; blood coagulation; sex differentiation; regulation of insulin secretion; phospholipid homeostasis

Disease: Fanconi Renotubular Syndrome 4 With Maturity-onset Diabetes Of The Young; Maturity-onset Diabetes Of The Young, Type 1; Diabetes Mellitus, Noninsulin-dependent