Rabbit anti-Human HN1 Polyclonal Antibody | anti-HN1 antibody

HN1, ID (HN1, ARM2, Hematological and neurological expressed 1 protein, Androgen-regulated protein 2) (APC)

Cat. Num. AAA6307408

• Gene Names: JPT1; HN1; ARM2; HN1A
• Reactivity: Human
• Applications: Flow Cytometry, Functional Assay, Immunofluorescence, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: HN1; Polyclonal Antibody; ID (HN1; ARM2; Hematological and neurological expressed 1 protein; Androgen-regulated protein 2) (APC); Androgen-regulated protein 2; anti-HN1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Allophycocyanin (APC).
• Sequence Length: 108

• Applicable Applications for anti-HN1 antibody: Flow Cytometry (FC/FACS), FLISA, Immunofluorescence (IF), Western Blot (WB)
• Application Notes: FC/FACS: 1:10-50; FLISA: 1:1,000; IF: 1:10-50; WB: 1:100-500; Applications are based on unconjugated antibody.
• Immunogen: HN1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 57-86 amino acids from the Central region of human HN1.
• Conjugate: APC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: APC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(HN1 Antibody (Center) western blot analysis in 293 cell line lysates (35ug/lane).This demonstrates the HN1 antibody detected the HN1 protein (arrow).)

Immunofluorescence (IF)

(Confocal immunofluorescent analysis of HN1 Antibody (Center) with A549 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red).DAPI was used to stain the cell nuclear (blue).)

Flow Cytometry (FC/FACS)

(HN1 Antibody (Center) flow cytometric analysis of A549 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Product Categories/Family for anti-HN1 antibody

Antibodies; Proteins

References

Laughlin, K.M., et al. Pathol. Oncol. Res. 15(3):437-444(2009). Sugiyama, N., et al. Mol. Cell Proteomics 6(6):1103-1109(2007). Olsen, J.V., et al. Cell 127(3):635-648(2006). Zougman, A., et al. J. Proteome Res. 5(4):925-934(2006). Zhou, G., et al. Gene 331, 115-123 (2004).

NCBI and Uniprot Product Information

• NCBI GI #: 50345292
• NCBI GeneID: 51155
• NCBI Accession #: NP_001002033.1; NP_057269.1
• NCBI GenBank Nucleotide #: NP_001002033.1
• UniProt Accession #: Q9UK76
• NCBI Official Full Name: jupiter microtubule associated homolog 1 isoform 3
• NCBI Official Synonym Full Names: Jupiter microtubule associated homolog 1
• NCBI Official Symbol: JPT1
• NCBI Official Synonym Symbols: HN1; ARM2; HN1A
• NCBI Protein Information: jupiter microtubule associated homolog 1
• UniProt Protein Name: Hematological and neurological expressed 1 protein
• Protein Family: Hematological and neurological expressed 1 protein
• UniProt Gene Name: HN1
• UniProt Synonym Gene Names: ARM2
• UniProt Entry Name: HN1_HUMAN

Uniprot Description

HN1: a nuclear protein of the HN1 family. Upregulated in advanced-stage epithelial ovarian cancer. Three alternatively spliced isoforms have been described.

Protein type: Unknown function

Chromosomal Location of Human Ortholog: 17q25.1

Cellular Component: nucleus

Biological Process: developmental process