Goat HMHA1 / HA1 Polyclonal Antibody | anti-HMHA1 antibody

Goat Polyclonal to Human HMHA1 / HA1

Cat. Num. AAA248940

• Gene Names: HMHA1; HA-1; HLA-HA1; ARHGAP45
• Reactivity: Bovine, Chimpanzee, Dog, Gorilla, Hamster, Human, Monkey, Orangutan
• Applications: Immunohistochemistry, ELISA
• Purity: Immunoaffinity Purified
• Synonyms: HMHA1 / HA1; Polyclonal Antibody; Goat Polyclonal to Human HMHA1 / HA1; Anti-HMHA1 / HA1 Antibody (aa57-71) IHC-plus; HMHA1; ARHGAP45; HLA-HA1; Ha-1; KIAA0223; HA1; Human HMHA1; anti-HMHA1 antibody

• Host: Goat
• Reactivity: Bovine, Chimpanzee, Dog, Gorilla, Hamster, Human, Monkey, Orangutan
• Clonality: Polyclonal
• Specificity: Human HMHA1 / HA-1. To avoid confusion please note that this antibody is designed to recognise the whole protein as coded by NP_036424.2 and will not recognise the peptide found eluted from HLA-A*0201 molecules by den Haan et al (Science. 1998 Feb 13;279(5353):1054-7, PMID: 9461441)
• Purity/Purification: Immunoaffinity Purified
• Form/Format: Tris-buffered saline, pH 7.3, 0.5% BSA, 0.02% sodium azide
• Concentration: 0.5 mg/ml (varies by lot)
• Sequence Length: 1152

• Applicable Applications for anti-HMHA1 antibody: Immunohistochemistry (IHC - Paraffin), ELISA (EIA)
• Application Notes: ELISA (1:128000), IHC-P (5 ug/ml); Usage: Peptide ELISA: antibody detection limit dilution 1:128000. Western blot: Preliminary experiments gave an approx 200kD band in lysates of cell lines Jurkat and Molt4 after 0.05 ug/ml antibody staining. Please note that currently we cannot find an expl...
• Target Species: Human
• Immunogen Description: Synthetic peptide KATGTLKRPTSLSRH-C from the N-terminus of human HMHA1 / HA-1 (NP_036424.2). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Orangutan, Monkey, Galago, Hamster, Panda, Bovine, Dog, Porcine (100%); Mouse, Rat (93%).
• Immunogen Type: Synthetic peptide
• Immunogen: HMHA1 / HA1 antibody was raised against synthetic peptide KATGTLKRPTSLSRH-C from the N-terminus of human HMHA1 / HA-1 (NP_036424.2). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Orangutan, Monkey, Galago, Hamster, Panda, Bovine, Dog, Porcine (100%); Mouse, Rat (93%); Opossum, Turkey, Chicken (87%); Zebra finch (80%).
• Antigen Modification: aa57-71
• Preparation and Storage: Store at -20 degree C. Minimize freezing and thawing.

Immunohistochemistry (IHC)

(Anti-HMHA1 / HA1 antibody IHC staining of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.)

NCBI and Uniprot Product Information

• NCBI GI #: 385251392
• NCBI GeneID: 23526
• NCBI Accession #: NP_001245257.1
• NCBI GenBank Nucleotide #: NM_001258328.2
• UniProt Accession #: Q92619; Q6P189; Q7LE26; Q86WS1; Q8HX84; Q9GJN9; Q9GJP0; Q9GJP1; Q9MY24; B4DTS4; F6QP70
• Molecular Weight: 126,497 Da
• NCBI Official Full Name: minor histocompatibility protein HA-1 isoform 2
• NCBI Official Synonym Full Names: histocompatibility (minor) HA-1
• NCBI Official Symbol: HMHA1
• NCBI Official Synonym Symbols: HA-1; HLA-HA1; ARHGAP45
• NCBI Protein Information: minor histocompatibility protein HA-1; minor histocompatibility antigen HA-1
• UniProt Protein Name: Minor histocompatibility protein HA-1
• Protein Family: Minor histocompatibility protein
• UniProt Gene Name: HMHA1
• UniProt Synonym Gene Names: KIAA0223; mHag HA-1
• UniProt Entry Name: HMHA1_HUMAN

Uniprot Description

HMHA1: GTPase activator for the Rho-type GTPases (Potential).

Protein type: GAPs; Cell surface; GAPs, Rac/Rho

Chromosomal Location of Human Ortholog: 19p13.3

Cellular Component: membrane; cytosol

Molecular Function: metal ion binding; GTPase activator activity

Biological Process: regulation of small GTPase mediated signal transduction; small GTPase mediated signal transduction; positive regulation of GTPase activity