Western Blot (WB)
(Western blot analysis of lysate from Jurkat cell line, using HMGXB3 Antibody (Center). MBS9203767 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysate at 35ug per lane.)
Rabbit anti-Human HMGXB3 Polyclonal Antibody | anti-HMGXB3 antibody
HMGXB3 Antibody (Center)
Gene Names
HMGXB3; SMF; HMGX3
Reactivity
Human
Applications
Western Blot, ELISA
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
HMGXB3; Polyclonal Antibody; HMGXB3 Antibody (Center); HMG domain-containing protein 3; HMG box-containing protein 3; Protein SMF; KIAA0194; SMF; anti-HMGXB3 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This HMGXB3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 879-908 amino acids from the Central region of human HMGXB3.
Purity/Purification
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Concentration
Vial Concentration: 0.49 (varies by lot)
Sequence Positions
879-908
Sequence Length
1,292
Applicable Applications for anti-HMGXB3 antibody
Western Blot (WB), ELISA (EIA)
Application Notes
WB~~1:1000
Antigen Type
Synthetic Peptide
Antigen Source
HUMAN
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
Western Blot (WB)
(Western blot analysis of lysate from Jurkat cell line, using HMGXB3 Antibody (Center). MBS9203767 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysate at 35ug per lane.)
Western Blot (WB)
(Western blot analysis of lysate from Jurkat cell line, using HMGXB3 Antibody (Center). MBS9203767 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysate at 35ug per lane.)
Related Product Information for anti-HMGXB3 antibody
HMGXB3 contains 1 HMG box DNA-binding domain.
References
Fellay, J., et al. PLoS Genet. 5 (12), E1000791 (2009) :
Andre, C., et al. Genomics 39(2):216-226(1997)
Gastier, J.M., et al. Genomics 32(1):75-85(1996)
Hampe, A., et al. Oncogene Res. 4(1):9-17(1989)
Andre, C., et al. Genomics 39(2):216-226(1997)
Gastier, J.M., et al. Genomics 32(1):75-85(1996)
Hampe, A., et al. Oncogene Res. 4(1):9-17(1989)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
168334
NCBI Official Full Name
HMG domain-containing protein 3
NCBI Official Synonym Full Names
HMG box domain containing 3
NCBI Official Symbol
HMGXB3
NCBI Official Synonym Symbols
SMF; HMGX3
NCBI Protein Information
HMG domain-containing protein 3
UniProt Protein Name
HMG domain-containing protein 3
Protein Family
UniProt Gene Name
HMGXB3
UniProt Synonym Gene Names
KIAA0194; SMF
UniProt Entry Name
HMGX3_HUMAN
NCBI Description
This gene is one of the non-canonical high mobility group (HMG) genes. The encoded protein contains an HMG-box domain found in DNA binding proteins such as transcription factors and chromosomal proteins. [provided by RefSeq, Aug 2011]
Uniprot Description
SMF: 2 isoforms of the human protein are produced by alternative splicing
Protein type: DNA-binding
Chromosomal Location of Human Ortholog: 5q32
Cellular Component: nucleus
Molecular Function: DNA binding; kinase activity
Biological Process: phosphorylation
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Product Notes
The HMGXB3 hmgxb3 (Catalog #AAA9203767) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 879-908. The HMGXB3 Antibody (Center) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's HMGXB3 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA). WB~~1:1000. Researchers should empirically determine the suitability of the HMGXB3 hmgxb3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "HMGXB3, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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