Rabbit anti-Human HMGCS2 Polyclonal Antibody | anti-HMGCS2 antibody

HMGCS2, CT (3-hydroxy-3-methylglutaryl Coenzyme A Synthase 2, Hydroxymethylglutaryl-CoA Synthase Mitochondrial, HMG-CoA Synthase) (MaxLight 405)

Cat. Num. AAA6481024

• Reactivity: Human
• Applications: Western Blot
• Purity: Purified by Ammonium Sulfate Precipitation.
• Synonyms: HMGCS2; Polyclonal Antibody; CT (3-hydroxy-3-methylglutaryl Coenzyme A Synthase 2; Hydroxymethylglutaryl-CoA Synthase Mitochondrial; HMG-CoA Synthase) (MaxLight 405); 3-hydroxy-3-methylglutaryl-CoA Synthase 2 (Mitochondrial); EC=2.3.3.10; anti-HMGCS2 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human HMGCS2.
• Purity/Purification: Purified by Ammonium Sulfate Precipitation.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight405.

• Applicable Applications for anti-HMGCS2 antibody: Western Blot (WB), FLISA
• Application Notes: WB: 1:50-1:100; FLISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: Synthetic peptide selected from the C-terminal region of human HMGCS2 (KLH).
• Conjugate: MaxLight405
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight405 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western blot analysis of HMGCS2 Antibody (C-term) in HepG2 cell line lysates (35ug/lane). HMGCS2 (arrow) was detected using the purified Pab.)

Immunohistochemistry (IHC)

(HMGCS2 Antibody (C-term) IHC analysis in formalin fixed and paraffin embedded human hepatocarcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the HMGCS2 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)

Flow Cytometry (FC/FACS)

(HMGCS2 Antibody (C-term) flow cytometric analysis of HepG2 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Product Categories/Family for anti-HMGCS2 antibody

Antibodies; Coenzyme A

References

1. Lu, Y., et.al., J. Lipid Res. 49(12), 2582-2589 (2008).

NCBI and Uniprot Product Information

• NCBI GI #: 5031751
• NCBI GeneID: 3158
• NCBI Accession #: NP_005509
• NCBI GenBank Nucleotide #: NM_005518
• UniProt Accession #: P54868
• Molecular Weight: 56kDa
• NCBI Official Full Name: hydroxymethylglutaryl-CoA synthase, mitochondrial isoform 1
• NCBI Official Synonym Full Names: 3-hydroxy-3-methylglutaryl-CoA synthase 2
• NCBI Official Symbol: HMGCS2
• NCBI Protein Information: hydroxymethylglutaryl-CoA synthase, mitochondrial
• UniProt Protein Name: Hydroxymethylglutaryl-CoA synthase, mitochondrial
• UniProt Gene Name: HMGCS2
• UniProt Synonym Gene Names: HMG-CoA synthase
• UniProt Entry Name: HMCS2_HUMAN

NCBI Description

The protein encoded by this gene belongs to the HMG-CoA synthase family. It is a mitochondrial enzyme that catalyzes the first reaction of ketogenesis, a metabolic pathway that provides lipid-derived energy for various organs during times of carbohydrate deprivation, such as fasting. Mutations in this gene are associated with HMG-CoA synthase deficiency. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Oct 2009]