Western Blot (WB)
(Western Blot analysis of 231 cell and Human fetal liver tissue using HMGB4 Polyclonal Antibody at dilution of 1:500)
Rabbit HMGB4 Polyclonal Antibody | anti-HMGB4 antibody
HMGB4 Polyclonal Antibody
Gene Names
HMGB4; dJ1007G16.5
Reactivity
Human, Mouse, Rat
Applications
ELISA, Western Blot
Purity
Antigen affinity purification
Synonyms
HMGB4; Polyclonal Antibody; HMGB4 Polyclonal Antibody; dJ1007G16.5; anti-HMGB4 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Antigen affinity purification
Concentration
0.7mg/mL (varies by lot)
Sequence Length
186
Applicable Applications for anti-HMGB4 antibody
ELISA (EIA), Western Blot (WB)
Application Notes
WB: 1:200-1:1000
Immunogen
Recombinant protein of human HMGB4
Buffer
PBS with 0.05% sodium azide, 50% glycerol, pH7.3
Preparation and Storage
Store at -20 degree C (regular) and -80 degree C (long term). Avoid freeze / thaw cycles.
Western Blot (WB)
(Western Blot analysis of 231 cell and Human fetal liver tissue using HMGB4 Polyclonal Antibody at dilution of 1:500)
Western Blot (WB)
(Western Blot analysis of 231 cell and Human fetal liver tissue using HMGB4 Polyclonal Antibody at dilution of 1:500)
Related Product Information for anti-HMGB4 antibody
High mobility group protein B4 is a transcription factor that in humans is encoded by the HMGB4 gene. HMGB4 is strongly and preferentially expressed in the adult mouse testis and weakly in the brain, but not in many other tissues. HMGB4 associates with chromatin, and in transfection assays, in contrast to HMGB1, it acts as a potent transcriptional repressor. During spermatogenesis, HMGB4 is present in the euchromatin of late pachytene spermatocytes and haploid round spermatids, whereas stronger expression is observed during the elongation phase, where it localizes to the basal pole of the nucleus in a manner mutually exclusive with H1FNT (H1T2) localized at the apical pole. HMGB4 basal localization is lost in H1FNT-mutant spermatids, showing that H1FNT provides a positional cue for organizing chromatin domains within the nucleus. These results show that HMGB4 and H1FNT specify distinct chromatin domains at the apical and basal poles of the elongating spermatid nucleus.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
22,490 Da
NCBI Official Full Name
High mobility group protein B4
NCBI Official Synonym Full Names
high mobility group box 4
NCBI Official Symbol
HMGB4
NCBI Official Synonym Symbols
dJ1007G16.5
NCBI Protein Information
high mobility group protein B4; HMG2 like
UniProt Protein Name
High mobility group protein B4
Protein Family
UniProt Gene Name
HMGB4
UniProt Entry Name
HMGB4_HUMAN
Uniprot Description
HMGB4: Belongs to the HMGB family.
Chromosomal Location of Human Ortholog: 1p35.1
Cellular Component: chromosome; nucleus
Molecular Function: DNA binding; chromatin binding
Biological Process: chromatin remodeling; regulation of transcription, DNA-dependent
Research Articles on HMGB4
Similar Products
Product Notes
The HMGB4 hmgb4 (Catalog #AAA2520237) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The HMGB4 Polyclonal Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's HMGB4 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB). WB: 1:200-1:1000. Researchers should empirically determine the suitability of the HMGB4 hmgb4 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "HMGB4, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
