Western Blot (WB)
(HMG-1 pAb tested by Western blot. Detection of trimethylated HMG-1 by Western blot. The analysis was performed using 20 ug HeLa acid extract and HMG-1 pAb at a 1:2,000 dilution.)
Rabbit anti-Human HMG-1 Polyclonal Antibody | anti-HMG-1 antibody
HMG-1 antibody (pAb)
WB: 1:500-1:2,000 dilution
Western Blot (WB)
(HMG-1 pAb tested by Western blot. Detection of trimethylated HMG-1 by Western blot. The analysis was performed using 20 ug HeLa acid extract and HMG-1 pAb at a 1:2,000 dilution.)
Western Blot (WB)
(HMG-1 pAb tested by Western blot. Detection of trimethylated HMG-1 by Western blot. The analysis was performed using 20 ug HeLa acid extract and HMG-1 pAb at a 1:2,000 dilution.)
Background: High mobility group proteins (HMG) are non-histone chromatin-associated proteins involved in DNA organization or regulation of transcription. Constitutively expressed in the nucleus of eukaryotic cells, HMGs share functional motifs that bind specific DNA structures (minor grooves) and induce conformational changes without specificity for target sequences. A Class II HMG member, HMG-1 binds to bent or distorted DNA such as four-way DNA junctions, synthetic DNA cruciforms, kinked cisplatin-modified DNA, DNA bulges and cross-overs in supercoiled DNA. It can cause looping of linear DNA, thus functioning as an architectural element that facilitates interactions of neighboring proteins and DNA by creating favorable DNA conformations. Extremely versatile, HMG-1 and HMG-2 are involved in numerous DNA events in the nucleus such as DNA repair, replication, recombination and transcription. Its nuclear localization and affinity for DNA is regulated through phosphorylation and acetylation, and has been found to have a dynamic relationship with chromatin. HMG-1 has been shown to stimulate the binding of a variety of sequence-specific DNA-binding proteins such as p53 in vitro, playing an important role in DNA architecture and transcriptional regulation.
NCBI and Uniprot Product Information
Observed: 24 kDa
Uniprot Description
HMGB1: DNA binding proteins that associates with chromatin and has the ability to bend DNA. Binds preferentially single-stranded DNA. Involved in V(D)J recombination by acting as a cofactor of the RAG complex. Acts by stimulating cleavage and RAG protein binding at the 23 bp spacer of conserved recombination signal sequences (RSS). Heparin-binding protein that has a role in the extension of neurite-type cytoplasmic processes in developing cells. Component of the RAG complex composed of core components RAG1 and RAG2, and associated component HMGB1 or HMGB2. Belongs to the HMGB family.
Protein type: DNA repair, damage; Nuclear receptor co-regulator
Chromosomal Location of Human Ortholog: 13q12
Cellular Component: nucleoplasm; extracellular space; cell surface; transcriptional repressor complex; extracellular region; condensed chromosome; nucleus
Molecular Function: protein binding; RAGE receptor binding; double-stranded DNA binding; damaged DNA binding; cytokine activity; chromatin binding; transcription factor activity; DNA bending activity; transcription factor binding; single-stranded DNA binding; chemoattractant activity
Biological Process: negative regulation of transcriptional preinitiation complex assembly; DNA topological change; V(D)J recombination; apoptosis; positive regulation of apoptosis; positive regulation of caspase activity; base-excision repair, DNA ligation; negative regulation of transcription from RNA polymerase II promoter; DNA recombination; chromatin remodeling; regulation of transcription from RNA polymerase II promoter; myeloid dendritic cell activation; inflammatory response to antigenic stimulus; positive chemotaxis; dendritic cell chemotaxis; innate immune response; DNA ligation during DNA repair; positive regulation of transcription from RNA polymerase II promoter; DNA fragmentation during apoptosis; neurite development; cell structure disassembly during apoptosis; positive regulation of DNA binding
Research Articles on HMG-1
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Product Notes
The HMG-1 hmgb1 (Catalog #AAA388487) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The HMG-1 antibody (pAb) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's HMG-1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). Applications Validated: WB: 1:500-1:2,000 dilution. Researchers should empirically determine the suitability of the HMG-1 hmgb1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "HMG-1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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