Rabbit anti-Human HMFG Polyclonal Antibody | anti-HMFG antibody

HMFG, CT (MFGE8, Lactadherin, Breast epithelial antigen BA46, HMFG, MFGM, Milk fat globule-EGF factor 8, SED1, Lactadherin short form, Medin) (AP)

Cat. Num. AAA6307198

• Gene Names: MFGE8; BA46; HMFG; MFGM; SED1; hP47; EDIL1; MFG-E8; SPAG10; OAcGD3S; HsT19888
• Reactivity: Human
• Applications: Western Blot, ELISA
• Purity: Purified by Protein A and Peptide Affinity Chromatography.
• Synonyms: HMFG; Polyclonal Antibody; CT (MFGE8; Lactadherin; Breast epithelial antigen BA46; MFGM; Milk fat globule-EGF factor 8; SED1; Lactadherin short form; Medin) (AP); MFGE8; Medin; anti-HMFG antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human HMFG.
• Purity/Purification: Purified by Protein A and Peptide Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Alkaline Phosphatase (AP).

• Applicable Applications for anti-HMFG antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: Applications are based on unconjugated antibody.
• Immunogen: KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 316-345 in the C-terminal region of human HMFG, Swiss/UniProt Accession: Q08431
• Conjugate: AP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western Blot analysis in Hela cell line lysates (35ug/lane) usingMBS6002952. This demonstrates that MBS6002952 detected the HMFG protein (arrow).)

Testing Data

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Related Product Information for anti-HMFG antibody

HMFG plays an important role in the maintenance of intestinal epithelial homeostasis and the promotion of mucosal healing. Promotes VEGF-dependent neovascularization (By similarity). Contributes to phagocytic removal of apoptotic cells in many tissues. Specific ligand for the alpha-v/beta-3 and alpha-v/beta-5 receptors. Also binds to phosphatidylserine-enriched cell surfaces in a receptor-independent manner. Zona pellucida-binding protein which may play a role in gamete interaction. Binds specifically to rotavirus and inhibits its replication. Medin is the main constituent of aortic medial amyloid.

Product Categories/Family for anti-HMFG antibody

Antibodies; Proteins

References

1. Yamaguchi, H., et al. Eur. J. Immunol. 40(6):1778-1785(2010). 2. Wheeler, H.E., et al. PLoS Genet. 5 (10), E1000685 (2009). 3. Hu, C.Y., et al. Lupus 18(8):676-681(2009). 4. Fu, Z., et al. Circ. Res. 104(12):1337-1346(2009). 5. Raymond, A., et al. J. Cell. Biochem. 106(6):957-966(2009).

NCBI and Uniprot Product Information

• NCBI GI #: 167830475
• NCBI GeneID: 4240
• NCBI Accession #: NP_005919.2; NP_001108086.1
• NCBI GenBank Nucleotide #: NM_005928.2
• UniProt Accession #: Q08431; Q53FU9; Q7Z3D2; Q9BTL9; B2R6M7
• Molecular Weight: 37,523 Da
• NCBI Official Full Name: lactadherin isoform a preproprotein
• NCBI Official Synonym Full Names: milk fat globule-EGF factor 8 protein
• NCBI Official Symbol: MFGE8
• NCBI Official Synonym Symbols: BA46; HMFG; MFGM; SED1; hP47; EDIL1; MFG-E8; SPAG10; OAcGD3S; HsT19888
• NCBI Protein Information: lactadherin; medin; sperm surface protein hP47; sperm associated antigen 10; breast epithelial antigen BA46; O-acetyl disialoganglioside synthase
• UniProt Protein Name: Lactadherin
• UniProt Gene Name: MFGE8
• UniProt Synonym Gene Names: MFG-E8
• UniProt Entry Name: MFGM_HUMAN

NCBI Description

This gene encodes a preproprotein that is proteolytically processed to form multiple protein products. The major encoded protein product, lactadherin, is a membrane glycoprotein that promotes phagocytosis of apoptotic cells. This protein has also been implicated in wound healing, autoimmune disease, and cancer. Lactadherin can be further processed to form a smaller cleavage product, medin, which comprises the major protein component of aortic medial amyloid (AMA). Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2015]

Uniprot Description

MFGE8: Plays an important role in the maintenance of intestinal epithelial homeostasis and the promotion of mucosal healing. Promotes VEGF-dependent neovascularization. Contributes to phagocytic removal of apoptotic cells in many tissues. Specific ligand for the alpha-v/beta-3 and alpha-v/beta-5 receptors. Also binds to phosphatidylserine-enriched cell surfaces in a receptor-independent manner. Zona pellucida-binding protein which may play a role in gamete interaction. Binds specifically to rotavirus and inhibits its replication. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 15q25

Cellular Component: extrinsic to plasma membrane; extracellular matrix; extracellular space; membrane; extracellular region; vesicle; external side of plasma membrane

Molecular Function: integrin binding; phosphatidylethanolamine binding; phosphatidylserine binding

Biological Process: viral reproduction; single fertilization; phagocytosis, recognition; angiogenesis; phagocytosis, engulfment; cell adhesion