Rabbit anti-Human HLA-DRB4 Polyclonal Antibody | anti-HLA-DRB4 antibody

HLA-DRB4, NT (HLA-DRB4, HLA class II histocompatibility antigen, DR beta 4 chain, MHC class II antigen DRB4) (PE)

Cat. Num. AAA6307142

• Gene Names: HLA-DRB4; DR13; DR14; DRB4; DR-13; DR-14; HLA-DR4B; HLA-DRB4
• Reactivity: Human
• Applications: Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: HLA-DRB4; Polyclonal Antibody; NT (HLA-DRB4; HLA class II histocompatibility antigen; DR beta 4 chain; MHC class II antigen DRB4) (PE); MHC class II antigen DRB4; anti-HLA-DRB4 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human HLA-DRB4.
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).

• Applicable Applications for anti-HLA-DRB4 antibody: FLISA, Western Blot (WB)
• Application Notes: Applications are based on unconjugated antibody.
• Immunogen: KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 43-72 in the N-terminal region of human HLA-DRB4, UniProt Accession #P13762.
• Conjugate: PE
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western blot analysis of HLA-DRB4 (arrow) usingMBS647312. 293 cell lysates (2ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the HLA-DRB4 gene.)

Product Categories/Family for anti-HLA-DRB4 antibody

Antibodies; HLA

References

Awa, W.L., et al. Eur. J. Endocrinol. 163(1):97-104(2010). Christiansen, O.B., et al. J. Reprod. Immunol. 85(1):9-14(2010). Amoli, M.M., et al. Dis. Markers 28(1):49-53(2010). Gomez-Tortosa, E., et al. Dement Geriatr Cogn Disord 30(1):8-11(2010). Wei, B., et al. Zhonghua Liu Xing Bing Xue Za Zhi 30(11):1143-1146(2009).

NCBI and Uniprot Product Information

• NCBI GI #: 52630344
• NCBI GeneID: 3126
• UniProt Accession #: P13762; O78042; P79664; Q29889; Q30163; Q6TLK6; Q860N4; Q861F3; Q8WLT9; Q9BS54; B0S863
• NCBI Official Full Name: major histocompatibility complex, class II, DR beta 4 [Homo sapiens]
• NCBI Official Synonym Full Names: major histocompatibility complex, class II, DR beta 4
• NCBI Official Symbol: HLA-DRB4
• NCBI Official Synonym Symbols: DR13; DR14; DRB4; DR-13; DR-14; HLA-DR4B; HLA-DRB4
• UniProt Protein Name: HLA class II histocompatibility antigen, DR beta 4 chain
• Protein Family: HLA class II histocompatibility antigen
• UniProt Gene Name: HLA-DRB4
• UniProt Entry Name: DRB4_HUMAN

Uniprot Description

Function: Binds peptides derived from antigens that access the endocytic route of antigen presenting cells (APC) and presents them on the cell surface for recognition by the CD4 T-cells. The peptide binding cleft accommodates peptides of 10-30 residues. The peptides presented by MHC class II molecules are generated mostly by degradation of proteins that access the endocytic route, where they are processed by lysosomal proteases and other hydrolases. Exogenous antigens that have been endocytosed by the APC are thus readily available for presentation via MHC II molecules, and for this reason this antigen presentation pathway is usually referred to as exogenous. As membrane proteins on their way to degradation in lysosomes as part of their normal turn-over are also contained in the endosomal/lysosomal compartments, exogenous antigens must compete with those derived from endogenous components. Autophagy is also a source of endogenous peptides, autophagosomes constitutively fuse with MHC class II loading compartments. In addition to APCs, other cells of the gastrointestinal tract, such as epithelial cells, express MHC class II molecules and CD74 and act as APCs, which is an unusual trait of the GI tract. To produce a MHC class II molecule that presents an antigen, three MHC class II molecules (heterodimers of an alpha and a beta chain) associate with a CD74 trimer in the ER to form a heterononamer. Soon after the entry of this complex into the endosomal/lysosomal system where antigen processing occurs, CD74 undergoes a sequential degradation by various proteases, including CTSS and CTSL, leaving a small fragment termed CLIP (class-II-associated invariant chain peptide). The removal of CLIP is facilitated by HLA-DM via direct binding to the alpha-beta-CLIP complex so that CLIP is released. HLA-DM stabilizes MHC class II molecules until primary high affinity antigenic peptides are bound. The MHC II molecule bound to a peptide is then transported to the cell membrane surface. In B-cells, the interaction between HLA-DM and MHC class II molecules is regulated by HLA-DO. Primary dendritic cells (DCs) also to express HLA-DO. Lysosomal microenvironment has been implicated in the regulation of antigen loading into MHC II molecules, increased acidification produces increased proteolysis and efficient peptide loading.

Subunit structure: Heterodimer of an alpha and a beta subunit; also referred as MHC class II molecule. In the endoplasmic reticulum (ER) it forms a heterononamer; 3 MHC class II molecules bind to a CD74 homotrimer (also known as invariant chain or HLA class II histocompatibility antigen gamma chain). In the endosomal/lysosomal system; CD74 undergoes sequential degradation by various proteases; leaving a small fragment termed CLIP on each MHC class II molecule. MHC class II molecule interacts with HLA_DM, and HLA_DO in B-cells, in order to release CLIP and facilitate the binding of antigenic peptides.

Subcellular location: Cell membrane; Single-pass type I membrane protein. Endoplasmic reticulum membrane; Single-pass type I membrane protein. Golgi apparatus › trans-Golgi network membrane; Single-pass type I membrane protein. Endosome membrane; Single-pass type I membrane protein. Lysosome membrane; Single-pass type I membrane protein. Late endosome membrane; Single-pass type I membrane protein. Note: The MHC class II complex transits through a number of intracellular compartments in the endocytic pathway until it reaches the cell membrane for antigen presentation. Ref.19 Ref.20

Post-translational modification: Ubiquitinated by MARCH1 and MARCH8 at Lys-254 leading to sorting into the endosome system and down-regulation of MHC class II. When associated with ubiquitination of the alpha subunit of HLA-DR: HLA-DRA 'Lys-244', the down-regulation of MHC class II may be highly effective. Ref.19 Ref.20

Polymorphism: The following alleles of DRB4 are known: DRB4*01:01, DRB4*01:02, DRB4*01:03, DRB4*01:04, DRB4*01:05, DRB4*01:06 and DRB4*01:07. The sequence shown is that of DRB4*01:03.

Sequence similarities: Belongs to the MHC class II family.Contains 1 Ig-like C1-type (immunoglobulin-like) domain.

Caution: HLA-DRB3, HLA-DRB4 and HLA-DRB5 may represent a unique gene.