Rabbit HLA-DPB1 Polyclonal Antibody | anti-HLA-DPB1 antibody

HLA-DPB1 Rabbit pAb

Cat. Num. AAA9142796

• Gene Names: HLA-DPB1; DPB1; HLA-DP; HLA-DPB; HLA-DP1B
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry, Immunofluorescence
• Purity: Affinity purification
• Synonyms: HLA-DPB1; Polyclonal Antibody; HLA-DPB1 Rabbit pAb; DPB1; HLA-DP; HLA-DP1B; HLA-DPB; major histocompatibility complex; class II; DP beta 1; anti-HLA-DPB1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Affinity purification
• Form/Format: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

• Applicable Applications for anti-HLA-DPB1 antibody: Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
• Application Notes: WB: 1:500-1:2000; IHC: 1:50-1:100; IF: 1:50-1:200
• Immunogen: Recombinant fusion protein of Human HLA-DPB1.
• Cellular Location: Cell membrane, Endoplasmic reticulum membrane, Endosome membrane, Golgi apparatus, Lysosome membrane, Single-pass type I membrane protein, trans-Golgi network membrane
• Positive Samples: U-937, A-549, Mouse spleen, Mouse thymus, Rat thymus
• Preparation and Storage: Store at -20 degree C. Avoid freeze/thaw cycles.

Western Blot (WB)

(Western blot analysis of extracts of various cell lines, using HLA-DPB1 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (RM00020).Exposure time: 1s.)

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded Human liver cancer using HLA-DPB1 antibody at dilution of 1:100 (40x lens).)

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded Mouse spleen using HLA-DPB1 antibody at dilution of 1:100 (40x lens).)

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded Rat spleen using HLA-DPB1 antibody at dilution of 1:100 (40x lens).)

Immunofluorescence (IF)

(Immunofluorescence analysis of C6 cells using HLA-DPB1 antibody at dilution of 1:100. Blue: DAPI for nuclear staining.)

Immunofluorescence (IF)

(Immunofluorescence analysis of HeLa cells using HLA-DPB1 antibody at dilution of 1:100. Blue: DAPI for nuclear staining.)

Immunofluorescence (IF)

(Immunofluorescence analysis of NIH/3T3 cells using HLA-DPB1 antibody at dilution of 1:100. Blue: DAPI for nuclear staining.)

Related Product Information for anti-HLA-DPB1 antibody

Background: HLA-DPB belongs to the HLA class II beta chain paralogues. This class II molecule is a heterodimer consisting of an alpha (DPA) and a beta chain (DPB), both anchored in the membrane. It plays a central role in the immune system by presenting peptides derived from extracellular proteins. Class II molecules are expressed in antigen presenting cells (APC: B lymphocytes, dendritic cells, macrophages). The beta chain is approximately 26-28 kDa and its gene contains 6 exons. Exon one encodes the leader peptide, exons 2 and 3 encode the two extracellular domains, exon 4 encodes the transmembrane domain and exon 5 encodes the cytoplasmic tail. Within the DP molecule both the alpha chain and the beta chain contain the polymorphisms specifying the peptide binding specificities, resulting in up to 4 different molecules. [provided by RefSeq, Jul 2008]

NCBI and Uniprot Product Information

• NCBI GI #: 122263
• NCBI GeneID: 3115
• UniProt Accession #: P04440; A0PFJ7; A5I886; A8YPB3; B5U8B4; B7VF80; B7VF87; B8ZX68; B8ZYT0; B9W5S8; B9W6F7; B9W6F9
• Molecular Weight: 258
• NCBI Official Full Name: HLA class II histocompatibility antigen, DP beta 1 chain
• NCBI Official Synonym Full Names: major histocompatibility complex, class II, DP beta 1
• NCBI Official Symbol: HLA-DPB1
• NCBI Official Synonym Symbols: DPB1; HLA-DP; HLA-DPB; HLA-DP1B
• NCBI Protein Information: HLA class II histocompatibility antigen, DP beta 1 chain; MHC HLA DPB1; HLA DP14-beta chain; MHC class II HLA-DRB1; class II HLA beta chain; MHC class II antigen DPB1; MHC class II HLA-DP-beta-1; MHC class II antigen DPbeta1; MHC class II antigen beta cha
• UniProt Protein Name: HLA class II histocompatibility antigen, DP beta 1 chain
• Protein Family: HLA class II histocompatibility antigen
• UniProt Gene Name: HLA-DPB1
• UniProt Synonym Gene Names: HLA-DP1B
• UniProt Entry Name: DPB1_HUMAN

NCBI Description

HLA-DPB belongs to the HLA class II beta chain paralogues. This class II molecule is a heterodimer consisting of an alpha (DPA) and a beta chain (DPB), both anchored in the membrane. It plays a central role in the immune system by presenting peptides derived from extracellular proteins. Class II molecules are expressed in antigen presenting cells (APC: B lymphocytes, dendritic cells, macrophages). The beta chain is approximately 26-28 kDa and its gene contains 6 exons. Exon one encodes the leader peptide, exons 2 and 3 encode the two extracellular domains, exon 4 encodes the transmembrane domain and exon 5 encodes the cytoplasmic tail. Within the DP molecule both the alpha chain and the beta chain contain the polymorphisms specifying the peptide binding specificities, resulting in up to 4 different molecules. [provided by RefSeq, Jul 2008]

Uniprot Description

HLA-DPB1: Binds peptides derived from antigens that access the endocytic route of antigen presenting cells (APC) and presents them on the cell surface for recognition by the CD4 T-cells. The peptide binding cleft accommodates peptides of 10-30 residues. The peptides presented by MHC class II molecules are generated mostly by degradation of proteins that access the endocytic route, where they are processed by lysosomal proteases and other hydrolases. Exogenous antigens that have been endocytosed by the APC are thus readily available for presentation via MHC II molecules, and for this reason this antigen presentation pathway is usually referred to as exogenous. As membrane proteins on their way to degradation in lysosomes as part of their normal turn-over are also contained in the endosomal/lysosomal compartments, exogenous antigens must compete with those derived from endogenous components. Autophagy is also a source of endogenous peptides, autophagosomes constitutively fuse with MHC class II loading compartments. In addition to APCs, other cells of the gastrointestinal tract, such as epithelial cells, express MHC class II molecules and CD74 and act as APCs, which is an unusual trait of the GI tract. To produce a MHC class II molecule that presents an antigen, three MHC class II molecules (heterodimers of an alpha and a beta chain) associate with a CD74 trimer in the ER to form a heterononamer. Soon after the entry of this complex into the endosomal/lysosomal system where antigen processing occurs, CD74 undergoes a sequential degradation by various proteases, including CTSS and CTSL, leaving a small fragment termed CLIP (class-II-associated invariant chain peptide). The removal of CLIP is facilitated by HLA-DM via direct binding to the alpha-beta-CLIP complex so that CLIP is released. HLA-DM stabilizes MHC class II molecules until primary high affinity antigenic peptides are bound. The MHC II molecule bound to a peptide is then transported to the cell membrane surface. In B-cells, the interaction between HLA-DM and MHC class II molecules is regulated by HLA-DO. Primary dendritic cells (DCs) also to express HLA-DO. Lysosomal miroenvironment has been implicated in the regulation of antigen loading into MHC II molecules, increased acidification produces increased proteolysis and efficient peptide loading. Belongs to the MHC class II family.

Protein type: Membrane protein, integral

Chromosomal Location of Human Ortholog: 6p21.3

Cellular Component: Golgi membrane; cell surface; membrane; lysosomal membrane; plasma membrane; endosome membrane; trans-Golgi network membrane; MHC class II protein complex

Molecular Function: peptide antigen binding

Biological Process: positive regulation of interferon-gamma production; cytokine and chemokine mediated signaling pathway; T cell costimulation; antigen processing and presentation of exogenous peptide antigen via MHC class II; positive regulation of T cell proliferation; positive regulation of T cell activation; T cell receptor signaling pathway