Rabbit anti-Human HLA-DMB Polyclonal Antibody | anti-HLA-DMB antibody

HLA-DMB, ID (HLA-DMB, DMB, RING7, HLA class II histocompatibility antigen, DM beta chain, MHC class II antigen DMB, Really interesting new gene 7 protein) (AP)

Cat. Num. AAA6307055

• Gene Names: HLA-DMB; RING7; D6S221E
• Reactivity: Human
• Applications: Western Blot, ELISA
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: HLA-DMB; Polyclonal Antibody; ID (HLA-DMB; DMB; RING7; HLA class II histocompatibility antigen; DM beta chain; MHC class II antigen DMB; Really interesting new gene 7 protein) (AP); Really interesting new gene 7 protein; anti-HLA-DMB antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Alkaline Phosphatase (AP).
• Sequence Length: 263

• Applicable Applications for anti-HLA-DMB antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:100-500; ELISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: HLA-DMB antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 103-133 amino acids from the Central region of human HLA-DMB.
• Conjugate: AP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(HLA-DMB Antibody (Center) western blot analysis in Ramos cell line lysates (35ug/lane).This demonstrates the HLA-DMB antibody detected the HLA-DMB protein (arrow).)

Product Categories/Family for anti-HLA-DMB antibody

Antibodies; HLA

References

Rinderknecht, C.H., et al. Immunology 131(1):18-32(2010). Bailey, S.D., et al. Diabetes Care (2010). In press: Davila, S., et al. Genes Immun. 11(3):232-238(2010). Ferrante, A., et al. J. Immunol. 184(3):1153-1158(2010). Talmud, P.J., et al. Am. J. Hum. Genet. 85(5):628-642(2009).

NCBI and Uniprot Product Information

• NCBI GI #: 4504399
• NCBI GeneID: 3109
• NCBI Accession #: NP_002109.1
• NCBI GenBank Nucleotide #: NP_002109.1
• UniProt Accession #: P28068
• NCBI Official Full Name: HLA class II histocompatibility antigen, DM beta chain
• NCBI Official Synonym Full Names: major histocompatibility complex, class II, DM beta
• NCBI Official Symbol: HLA-DMB
• NCBI Official Synonym Symbols: RING7; D6S221E
• NCBI Protein Information: HLA class II histocompatibility antigen, DM beta chain
• UniProt Protein Name: HLA class II histocompatibility antigen, DM beta chain
• Protein Family: HLA class II histocompatibility antigen
• UniProt Gene Name: HLA-DMB
• UniProt Synonym Gene Names: DMB; RING7
• UniProt Entry Name: DMB_HUMAN

NCBI Description

HLA-DMB belongs to the HLA class II beta chain paralogues. This class II molecule is a heterodimer consisting of an alpha (DMA) and a beta (DMB) chain, both anchored in the membrane. It is located in intracellular vesicles. DM plays a central role in the peptide loading of MHC class II molecules by helping to release the CLIP (class II-associated invariant chain peptide) molecule from the peptide binding site. Class II molecules are expressed in antigen presenting cells (APC: B lymphocytes, dendritic cells, macrophages). The beta chain is approximately 26-28 kDa and its gene contains 6 exons. Exon one encodes the leader peptide, exons 2 and 3 encode the two extracellular domains, exon 4 encodes the transmembrane domain and exon 5 encodes the cytoplasmic tail. [provided by RefSeq, Jul 2008]

Uniprot Description

HLA-DMB: Plays a critical role in catalyzing the release of class II-associated invariant chain peptide (CLIP) from newly synthesized MHC class II molecules and freeing the peptide binding site for acquisition of antigenic peptides. In B-cells, the interaction between HLA-DM and MHC class II molecules is regulated by HLA-DO. Belongs to the MHC class II family.

Protein type: Vesicle; Membrane protein, integral

Chromosomal Location of Human Ortholog: 6p21.3

Cellular Component: lysosomal membrane; late endosome membrane; integral to membrane; MHC class II protein complex

Biological Process: peptide antigen assembly with MHC class II protein complex; antigen processing and presentation of exogenous peptide antigen via MHC class II; positive regulation of T cell proliferation; immune response; MHC class II protein complex assembly