Rabbit anti-Human HLA-DMA Polyclonal Antibody | anti-HLA-DMA antibody

HLA-DMA antibody

Cat. Num. AAA9402705

• Gene Names: HLA-DMA; DMA; HLADM; RING6; D6S222E
• Reactivity: Human
• Applications: Western Blot, Immunohistochemistry
• Purity: Purified by antigen-affinity chromatography.
• Synonyms: HLA-DMA; Polyclonal Antibody; HLA-DMA antibody; anti-HLA-DMA antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Purity/Purification: Purified by antigen-affinity chromatography.
• Form/Format: Supplied in 0.1M Tris-buffered saline with 20% Glycerol (pH7.0). 0.01% Thimerosal was added as a preservative.
• Sequence Length: 261

• Applicable Applications for anti-HLA-DMA antibody: Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: Western blotting: 1:500-1:3000; Immunohistochemistry: 1:100-1:250
• Immunogen Type: Recombinant protein
• Immunogen Description: Recombinant protein fragment contain a sequence corresponding to a region within amino acids 52 and 255 of HLA-DMA
• Target Name: HLA-DMA
• Preparation and Storage: Store at -20 degree C for long term preservation (recommended). Store at 4 degree C for short term use.

Testing Data

(Sample (30 ug of whole cell lysate) A: Hela 12% SDS PAGE Primary antibody diluted at 1: 1000)

Immunohistochemistry (IHC)

(Immunohistochemical analysis of paraffin-embedded Breast ca, using HLA-DMA antibody at 1: 250 dilution.)

Related Product Information for anti-HLA-DMA antibody

HLA-DMA belongs to the HLA class II alpha chain paralogues. This class II molecule is a heterodimer consisting of an alpha (DMA) and a beta chain (DMB), both anchored in the membrane. It is located in intracellular vesicles. DM plays a central role in the peptide loading of MHC class II molecules by helping to release the CLIP molecule from the peptide binding site. Class II molecules are expressed in antigen presenting cells (APC: B lymphocytes, dendritic cells, macrophages). The alpha chain is approximately 33-35 kDa and its gene contains 5 exons. Exon one encodes the leader peptide, exons 2 and 3 encode the two extracellular domains, exon 4 encodes the transmembrane domain and the cytoplasmic tail. [provided by RefSeq]

Product Categories/Family for anti-HLA-DMA antibody

Total protein Ab

NCBI and Uniprot Product Information

• NCBI GI #: 15030336
• NCBI GeneID: 3108
• Molecular Weight: 29,194 Da
• NCBI Official Full Name: Major histocompatibility complex, class II, DM alpha
• NCBI Official Synonym Full Names: major histocompatibility complex, class II, DM alpha
• NCBI Official Symbol: HLA-DMA
• NCBI Official Synonym Symbols: DMA; HLADM; RING6; D6S222E
• NCBI Protein Information: HLA class II histocompatibility antigen, DM alpha chain
• UniProt Protein Name: HLA class II histocompatibility antigen, DM alpha chain
• Protein Family: HLA class II histocompatibility antigen
• UniProt Gene Name: HLA-DMA
• UniProt Synonym Gene Names: DMA; RING6
• UniProt Entry Name: DMA_HUMAN

NCBI Description

HLA-DMA belongs to the HLA class II alpha chain paralogues. This class II molecule is a heterodimer consisting of an alpha (DMA) and a beta chain (DMB), both anchored in the membrane. It is located in intracellular vesicles. DM plays a central role in the peptide loading of MHC class II molecules by helping to release the CLIP molecule from the peptide binding site. Class II molecules are expressed in antigen presenting cells (APC: B lymphocytes, dendritic cells, macrophages). The alpha chain is approximately 33-35 kDa and its gene contains 5 exons. Exon one encodes the leader peptide, exons 2 and 3 encode the two extracellular domains, exon 4 encodes the transmembrane domain and the cytoplasmic tail. [provided by RefSeq, Jul 2008]

Uniprot Description

HLA-DMA: Plays a critical role in catalyzing the release of class II-associated invariant chain peptide (CLIP) from newly synthesized MHC class II molecules and freeing the peptide binding site for acquisition of antigenic peptides. In B-cells, the interaction between HLA-DM and MHC class II molecules is regulated by HLA-DO. Belongs to the MHC class II family.

Protein type: Membrane protein, integral; Chaperone

Chromosomal Location of Human Ortholog: 6p21.3

Cellular Component: multivesicular body; cell surface; membrane; late endosome membrane; lysosomal membrane; integral to membrane; MHC class II protein complex

Biological Process: protein transport; positive regulation of immune response; positive regulation of T cell differentiation; chaperone cofactor-dependent protein folding; peptide antigen assembly with MHC class II protein complex; antigen processing and presentation of exogenous peptide antigen via MHC class II; immunoglobulin mediated immune response; inner ear development; positive thymic T cell selection