Rabbit anti-Human HLA Class 2 Antigen DRB1 Polyclonal Antibody | anti-HLA-DR1B antibody

HLA Class 2 Antigen DRB1 (HLA Class II Histocompatibility Antigen DRB1-1 beta Chain, HLA-DR1B, HLA-DRB1, HLA-DRB1*, HLA-DRB, DR1, DR-1, DRB1, DRw10, FLJ75017, FLJ76359, MHC Class II Antigen DRB1*1, SS1) (FITC)

Cat. Num. AAA6381202

• Gene Names: HLA-DRB1; SS1; DRB1; HLA-DRB; HLA-DR1B
• Reactivity: Human
• Applications: Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: HLA Class 2 Antigen DRB1; Polyclonal Antibody; HLA Class 2 Antigen DRB1 (HLA Class II Histocompatibility Antigen DRB1-1 beta Chain; HLA-DR1B; HLA-DRB1; HLA-DRB1*; HLA-DRB; DR1; DR-1; DRB1; DRw10; FLJ75017; FLJ76359; MHC Class II Antigen DRB1*1; SS1) (FITC); anti-HLA-DR1B antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human HLA-DRB1.
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein Isothiocyanate (FITC).

• Applicable Applications for anti-HLA-DR1B antibody: Western Blot (WB)
• Application Notes: Applications are based on unconjugated antibody.
• Immunogen: Full length human HLA-DRB1, aa1-266 (AAH08403.1).
• Immunogen Sequence: MVCLRLPGGSCMAVLTVTLMVLSSPLALAGDTRPRFLEYSTSECHFFNGTERVRFLDRYFYNQEEYVRFDSDVGEYRAVTELGRPSAEYWNSQKDILEDKRAAVDTYCRHNYGVGESFTVQRRVHPKVTVYPSKTQPLQHHNLLVCSVSGFYPGSIEVRWFRNGQEEKTGVVSTGLIHNGDWTFQTLVMLETVPRSGEVYTCQVEHPSVTSPLTVEWRARSESAQSKMLSGVGGFVLGLLFLGAGLFIYFRNQKGHSGLQPRGFLS
• Conjugate: FITC
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western Blot analysis of HLA-DRB1 expression in transfected 293T cell line by HLA-DRB1 polyclonal antibody. Lane 1: HLA-DRB1 transfected lysate (30kD). Lane 2: Non-transfected lysate.)

Product Categories/Family for anti-HLA-DR1B antibody

Antibodies; HLA

NCBI and Uniprot Product Information

• NCBI GI #: 14250013
• NCBI GeneID: 3123
• Molecular Weight: 30,160 Da
• NCBI Official Full Name: Homo sapiens major histocompatibility complex, class II, DR beta 1, mRNA
• NCBI Official Synonym Full Names: major histocompatibility complex, class II, DR beta 1
• NCBI Official Symbol: HLA-DRB1
• NCBI Official Synonym Symbols: SS1; DRB1; HLA-DRB; HLA-DR1B
• NCBI Protein Information: major histocompatibility complex, class II, DR beta 1

NCBI Description

HLA-DRB1 belongs to the HLA class II beta chain paralogs. The class II molecule is a heterodimer consisting of an alpha (DRA) and a beta chain (DRB), both anchored in the membrane. It plays a central role in the immune system by presenting peptides derived from extracellular proteins. Class II molecules are expressed in antigen presenting cells (APC: B lymphocytes, dendritic cells, macrophages). The beta chain is approximately 26-28 kDa. It is encoded by 6 exons. Exon one encodes the leader peptide; exons 2 and 3 encode the two extracellular domains; exon 4 encodes the transmembrane domain; and exon 5 encodes the cytoplasmic tail. Within the DR molecule the beta chain contains all the polymorphisms specifying the peptide binding specificities. Hundreds of DRB1 alleles have been described and typing for these polymorphisms is routinely done for bone marrow and kidney transplantation. DRB1 is expressed at a level five times higher than its paralogs DRB3, DRB4 and DRB5. DRB1 is present in all individuals. Allelic variants of DRB1 are linked with either none or one of the genes DRB3, DRB4 and DRB5. There are 4 related pseudogenes: DRB2, DRB6, DRB7, DRB8 and DRB9. [provided by RefSeq, Jul 2008]