Goat HLA-B Polyclonal Antibody | anti-HLA-B antibody

Goat anti-HLA-B (aa132-143) Antibody

Cat. Num. AAA423594

• Gene Names: HLA-B; AS; HLAB; Bw-47; Bw-50; SPDA1
• Reactivity: Tested: Human; Expected from sequence similarity: Human
• Applications: ELISA, Western Blot
• Purity: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Synonyms: HLA-B; Polyclonal Antibody; Goat anti-HLA-B (aa132-143) Antibody; Goat Anti-HLA-B Antibody; major histocompatibility complex; class I; B; AS; HLAB; SPDA1; HLA class I histocompatibility antigen; B alpha chain; MHC Class I HLA heavy chain; MHC HLA-B cell surface glycoprotein; MHC HLA-B transmembrane glycoprotein; MHC class I antigen GN0010; HLA-B antibody; B antibody; AS antibody; HLAB antibody; SPDA1 antibody; B alpha chain antibody; MHC Class I HLA heavy chain antibody; MHC HLA-B cell surface glycoprotein antibody; MHC HLA-B transmembrane glycoprotein antibody; MHC class I antigen GN0010 antibody; HLA-B (aa132-143); anti-HLA-B antibody

• Host: Goat
• Reactivity: Tested: Human; Expected from sequence similarity: Human
• Clonality: Polyclonal
• Purity/Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Form/Format: Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
• Concentration: 100ug specific antibody in 200ul (varies by lot)
• Sequence: RLLRGHDQYAYD
• Sequence Length: 362

• Applicable Applications for anti-HLA-B antibody: Peptide ELISA (EIA), Western Blot (WB)
• Application Notes: Peptide ELISA: Antibody detection limit dilution 1: 128000.; Western Blot: Approx 40+45kDa bands observed in lysates of cell line Jurkat (calculated MW of 40.5kDa according to NP_005505.2). The observed 45kDa band corresponds to the glycosylated form. Recommended concentration: 0.1-0.3ug/ml.
• Immunogen: Peptide with sequence C-RLLRGHDQYAYD, from the internal region of the protein sequence according to NP_005505.2.
• Epitope: Internal region
• Preparation and Storage: Aliquot and store at -20 degree C. Minimize freezing and thawing.

Western Blot (WB)

((0.1ug/ml) staining of Jurkat lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)

NCBI and Uniprot Product Information

• NCBI GI #: 17986001
• NCBI GeneID: 3106
• NCBI Accession #: NP_005505.2
• NCBI GenBank Nucleotide #: NM_005514.7
• Molecular Weight: 40,456 Da
• NCBI Official Full Name: major histocompatibility complex, class I, B
• NCBI Official Synonym Full Names: major histocompatibility complex, class I, B
• NCBI Official Symbol: HLA-B
• NCBI Official Synonym Symbols: AS; HLAB; Bw-47; Bw-50; SPDA1
• NCBI Protein Information: major histocompatibility complex, class I, B
• UniProt Protein Name: HLA class I histocompatibility antigen, B-37 alpha chain
• Protein Family: HLA class I histocompatibility antigen
• UniProt Gene Name: HLA-B
• UniProt Synonym Gene Names: HLAB
• UniProt Entry Name: 1B37_HUMAN

NCBI Description

HLA-B belongs to the HLA class I heavy chain paralogues. This class I molecule is a heterodimer consisting of a heavy chain and a light chain (beta-2 microglobulin). The heavy chain is anchored in the membrane. Class I molecules play a central role in the immune system by presenting peptides derived from the endoplasmic reticulum lumen. They are expressed in nearly all cells. The heavy chain is approximately 45 kDa and its gene contains 8 exons. Exon 1 encodes the leader peptide, exon 2 and 3 encode the alpha1 and alpha2 domains, which both bind the peptide, exon 4 encodes the alpha3 domain, exon 5 encodes the transmembrane region and exons 6 and 7 encode the cytoplasmic tail. Polymorphisms within exon 2 and exon 3 are responsible for the peptide binding specificity of each class one molecule. Typing for these polymorphisms is routinely done for bone marrow and kidney transplantation. Hundreds of HLA-B alleles have been described. [provided by RefSeq, Jul 2008]

Uniprot Description

Involved in the presentation of foreign antigens to the immune system.