Western Blot (WB)
(HJURP Antibody (C-term) western blot analysis in Jurkat cell line lysates (35ug/lane).This demonstrates the HJURP antibody detected the HJURP protein (arrow).)
Rabbit anti-Human HJURP Polyclonal Antibody | anti-HJURP antibody
HJURP, CT (HJURP, FAKTS, FLEG1, URLC9, Holliday junction recognition protein, 14-3-3-associated AKT substrate, Fetal liver-expressing gene 1 protein, Up-regulated in lung cancer 9) (PE)
Gene Names
HJURP; FAKTS; URLC9; hFLEG1
Reactivity
Human
Applications
Flow Cytometry, Functional Assay, Immunohistochemistry, Western Blot
Purity
Purified by Protein A Affinity Chromatography.
Synonyms
HJURP; Polyclonal Antibody; CT (HJURP; FAKTS; FLEG1; URLC9; Holliday junction recognition protein; 14-3-3-associated AKT substrate; Fetal liver-expressing gene 1 protein; Up-regulated in lung cancer 9) (PE); Up-regulated in lung cancer 9; anti-HJURP antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Purified by Protein A Affinity Chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).
Applicable Applications for anti-HJURP antibody
Flow Cytometry (FC/FACS), FLISA, Immunohistochemistry (IHC), Western Blot (WB)
Application Notes
FC/FACS: 1:10-50
FLISA: 1:1,000
IHC: 1:10-50
WB: 1:100-500
Applications are based on unconjugated antibody.
FLISA: 1:1,000
IHC: 1:10-50
WB: 1:100-500
Applications are based on unconjugated antibody.
Immunogen
HJURP antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 558-588 amino acids from the C-terminal region of human HJURP.
Conjugate
PE
Note
Preservative Free
Special Handling
Light sensitive
Preparation and Storage
Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Western Blot (WB)
(HJURP Antibody (C-term) western blot analysis in Jurkat cell line lysates (35ug/lane).This demonstrates the HJURP antibody detected the HJURP protein (arrow).)
Western Blot (WB)
(HJURP Antibody (C-term) western blot analysis in Jurkat cell line lysates (35ug/lane).This demonstrates the HJURP antibody detected the HJURP protein (arrow).)
Immunohistochemistry (IHC)
(HJURP Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human thyroid tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of HJURP Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
Immunohistochemistry (IHC)
(HJURP Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human thyroid tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of HJURP Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
Flow Cytometry (FC/FACS)
(HJURP Antibody (C-term) flow cytometric analysis of Jurkat cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated donkey-anti-rabbit secondary antibodies were used for the analysis.)
Flow Cytometry (FC/FACS)
(HJURP Antibody (C-term) flow cytometric analysis of Jurkat cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated donkey-anti-rabbit secondary antibodies were used for the analysis.)
Related Product Information for anti-HJURP antibody
HJURP is a centromeric protein that plays a central role in the incorporation and maintenance of histone H3-like variant CENPA at centromeres. Acts as a specific chaperone for CENPA and is required for the incorporation of newly synthesized CENPA molecules into nucleosomes at replicated centromeres. Directly binds Holliday junctions.
Product Categories/Family for anti-HJURP antibody
References
Sebastiani, P., et al. Science (2010). In press: Shuaib, M., et al. Proc. Natl. Acad. Sci. U.S.A. 107(4):1349-1354(2010). Hu, Z., et al. Breast Cancer Res. 12 (2), R18 (2010): Dunleavy, E.M., et al. Cell 137(3):485-497(2009). Foltz, D.R., et al. Cell 137(3):472-484(2009).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
83,539 Da
NCBI Official Full Name
Holliday junction recognition protein isoform a
NCBI Official Synonym Full Names
Holliday junction recognition protein
NCBI Official Symbol
HJURP
NCBI Official Synonym Symbols
FAKTS; URLC9; hFLEG1
NCBI Protein Information
Holliday junction recognition protein; fetal liver expressing gene 1; up-regulated in lung cancer 9; 14-3-3-associated AKT substrate; fetal liver-expressing gene 1 protein
UniProt Protein Name
Holliday junction recognition protein
Protein Family
UniProt Gene Name
HJURP
UniProt Synonym Gene Names
FAKTS; FLEG1; URLC9
UniProt Entry Name
HJURP_HUMAN
Research Articles on HJURP
Similar Products
Product Notes
The HJURP hjurp (Catalog #AAA6307010) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The HJURP, CT (HJURP, FAKTS, FLEG1, URLC9, Holliday junction recognition protein, 14-3-3-associated AKT substrate, Fetal liver-expressing gene 1 protein, Up-regulated in lung cancer 9) (PE) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's HJURP can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC/FACS), FLISA, Immunohistochemistry (IHC), Western Blot (WB). FC/FACS: 1:10-50 FLISA: 1:1,000 IHC: 1:10-50 WB: 1:100-500 Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the HJURP hjurp for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "HJURP, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
