Rabbit anti-Human Histone H3 Polyclonal Antibody | anti-H3F3A antibody

Histone H3, Phosphorylated (Ser10) (H3 Histone, Family 3A) (FITC)

Cat. Num. AAA6480747

• Reactivity: Human
• Applications: Immunohistochemistry, ELISA, Western Blot
• Purity: Purified by Protein G Affinity Chromatography.
• Synonyms: Histone H3; Polyclonal Antibody; Phosphorylated (Ser10) (H3 Histone; Family 3A) (FITC); anti-H3F3A antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human histone H3 phosphorylated at Ser10.
• Purity/Purification: Purified by Protein G Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein Isothiocyanate (FITC).
• Sequence Length: 136

• Applicable Applications for anti-H3F3A antibody: Immunohistochemistry (IHC), ELISA (EIA), Western Blot (WB)
• Application Notes: IHC: 1:100; Applications are based on unconjugated antibody.
• Immunogen: Synthetic phosphopeptide corresponding to amino acid residues surrounding Ser10 of human histone cluster 1, H3B (MAPS).
• Conjugate: FITC
• Positive Control: Colcemid treated HeLa acid extracts
• Antigen Modification: Phosphorylated
• Note: Preservative Free
• Preparation and Storage: May be stored at 4 degree C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Immunohistochemistry (IHC)

(Immunohistochemistry: Representative lot data. Immunohistochemical analysis of paraffin-embedded human skin carcinoma using anti-phospho Histone H3(Ser10), 1:100 dilution. The antibody gave a strong nuclear signal in epithelial cells associated with blood vessels surrounding sebaceous glands in epidermal layer adjacent to tumor (left) and in malignant melanoma (right).)

Western Blot (WB)

(Western Blot Analysis: This antibody detected phosphohistone H3 in 10 ug of colcemid-treated HeLa acid extract.)

Product Categories/Family for anti-H3F3A antibody

Antibodies; Histones

References

1. Lusic, M., et al., EMBO J. 22(24): 6550-6561 (2003). 2. Deng, L., et al., Virology 289(2): 312-326 (2001). 3. Deng, L., et al., Virology 277(2): 278-295 (2000). 4. El Kharroubi, A., et al., Mol. Cell. Biol. 18(5): 2535-2544 (1998). 5. Albig, W., et al., Hum. Genet. 101(3): 284-294 (1997). 6p22-p21. 3.

NCBI and Uniprot Product Information

• NCBI GI #: 4504297
• NCBI Accession #: NP_003528
• NCBI GenBank Nucleotide #: NP_003528.1
• UniProt Accession #: P84243
• Molecular Weight: 17
• NCBI Official Full Name: histone H3.1
• UniProt Protein Name: Histone H3.3
• Protein Family: Histone
• UniProt Gene Name: H3F3A
• UniProt Synonym Gene Names: H3.3A; H3F3
• UniProt Entry Name: H33_HUMAN

Uniprot Description

H3F3A: Variant histone H3 which replaces conventional H3 in a wide range of nucleosomes in active genes. Constitutes the predominant form of histone H3 in non-dividing cells and is incorporated into chromatin independently of DNA synthesis. Deposited at sites of nucleosomal displacement throughout transcribed genes, suggesting that it represents an epigenetic imprint of transcriptionally active chromatin. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. The nucleosome is a histone octamer containing two molecules each of H2A, H2B, H3 and H4 assembled in one H3-H4 heterotetramer and two H2A-H2B heterodimers. The octamer wraps approximately 147 bp of DNA. Interacts with HIRA, a chaperone required for its incorporation into nucleosomes. Belongs to the histone H3 family.

Protein type: DNA-binding

Chromosomal Location of Human Ortholog: 1q42.12

Cellular Component: nucleoplasm; nuclear chromosome; protein complex; extracellular region; nucleosome; nucleus

Molecular Function: protein binding; nucleosomal DNA binding; protein heterodimerization activity

Biological Process: chromatin silencing at rDNA; nucleosome assembly; DNA replication-independent nucleosome assembly; negative regulation of gene expression, epigenetic; gene expression; blood coagulation; DNA methylation on cytosine; positive regulation of cell growth; regulation of gene expression, epigenetic