Rabbit anti-Human Histone H3 Polyclonal Antibody | anti-H3F3A antibody

Histone H3 (K9) (H3F3A, H3.3A, H3F3, Histone H3.3) (Azide free) (HRP)

Cat. Num. AAA6306993

• Gene Names: H3F3A; H3F3; H3.3A
• Reactivity: Human
• Applications: Immunohistochemistry, ELISA, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: Histone H3; Polyclonal Antibody; Histone H3 (K9) (H3F3A; H3.3A; H3F3; Histone H3.3) (Azide free) (HRP); H3F3A; Histone H3.3; anti-H3F3A antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).

• Applicable Applications for anti-H3F3A antibody: Immunohistochemistry (IHC), ELISA (EIA), Western Blot (WB)
• Application Notes: IHC: 1:50-100; ELISA: 1:1,000; WB: 1:100-500; Applications are based on unconjugated antibody.
• Immunogen: Histone H3-K9 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the K9 region of human histone H3.
• Conjugate: HRP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western blot analysis of Methyl-K-H3-K9(2Me)-4MAPS in K562 cell line lysates (35ug/lane). H3 (arrow) was detected using the purified Pab.)

Immunohistochemistry (IHC)

(Histone H3-K9 Antibody IHC analysis in formalin fixed and paraffin embedded testis tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the Histone H3-K9 Antibody for immunohistochemistry. Clinical relevance has not been evaluated.)

Flow Cytometry (FC/FACS)

(Histone H3-K9 Antibody flow cytometric analysis of K562 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Related Product Information for anti-H3F3A antibody

Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. This structure consists of approximately 146 bp of DNA wrapped around a nucleosome, an octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene for histone H3-MeK9 is intronless and encodes a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6p22-p21.3.

Product Categories/Family for anti-H3F3A antibody

Antibodies; Histones

References

Teng, H., et al. J. Biol. Chem. 284(39):26368-26376(2009). Garcia, B.A., et al. J. Biol. Chem. 282(10):7641-7655(2007). Morris, S.A., et al. J. Biol. Chem. 282(10):7632-7640(2007). Loyola, A., et al. Mol. Cell 24(2):309-316(2006). Kim, S.C., et al. Mol. Cell 23(4):607-618(2006).

NCBI and Uniprot Product Information

• NCBI GI #: 4504279
• NCBI GeneID: 3020
• NCBI Accession #: NP_002098.1
• NCBI GenBank Nucleotide #: NM_002107.4
• UniProt Accession #: P84243; P06351; P33155; Q5VV55; Q5VV56; Q66I33; Q9V3W4
• Molecular Weight: 15,328 Da
• NCBI Official Full Name: histone H3.3
• NCBI Official Synonym Full Names: H3 histone, family 3A
• NCBI Official Symbol: H3F3A
• NCBI Official Synonym Symbols: H3F3; H3.3A
• NCBI Protein Information: histone H3.3
• UniProt Protein Name: Histone H3.3
• Protein Family: Histone
• UniProt Gene Name: H3F3A
• UniProt Synonym Gene Names: H3.3A; H3F3
• UniProt Entry Name: H33_HUMAN

NCBI Description

Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene contains introns and its mRNA is polyadenylated, unlike most histone genes. The protein encoded is a replication-independent member of the histone H3 family. [provided by RefSeq, Jul 2008]

Uniprot Description

H3F3A: Variant histone H3 which replaces conventional H3 in a wide range of nucleosomes in active genes. Constitutes the predominant form of histone H3 in non-dividing cells and is incorporated into chromatin independently of DNA synthesis. Deposited at sites of nucleosomal displacement throughout transcribed genes, suggesting that it represents an epigenetic imprint of transcriptionally active chromatin. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. The nucleosome is a histone octamer containing two molecules each of H2A, H2B, H3 and H4 assembled in one H3-H4 heterotetramer and two H2A-H2B heterodimers. The octamer wraps approximately 147 bp of DNA. Interacts with HIRA, a chaperone required for its incorporation into nucleosomes. Belongs to the histone H3 family.

Protein type: DNA-binding

Chromosomal Location of Human Ortholog: 1q42.12

Cellular Component: nucleoplasm; nuclear chromosome; protein complex; extracellular region; nucleosome; nucleus

Molecular Function: protein binding; nucleosomal DNA binding; protein heterodimerization activity

Biological Process: chromatin silencing at rDNA; nucleosome assembly; DNA replication-independent nucleosome assembly; negative regulation of gene expression, epigenetic; gene expression; blood coagulation; DNA methylation on cytosine; positive regulation of cell growth; regulation of gene expression, epigenetic