Rabbit anti-Human Histone H3 Polyclonal Antibody | anti-HIST1H3A antibody

Rabbit anti Histone H3 (Acetylation at K9) Polyclonal Antibody

Cat. Num. AAA460508

• Gene Names: HIST1H3A; H3/A; H3FA
• Reactivity: Human
• Applications: ELISA, Flow Cytometry, Functional Assay, Immunohistochemistry, Immunoprecipitation, Western Blot
• Purity: Purified by site-modified Epitope Affinity Purification.
• Synonyms: Histone H3; Polyclonal Antibody; Rabbit anti Histone H3 (Acetylation at K9) Polyclonal Antibody; HIST1H3A; anti-HIST1H3A antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: This antibody only recognizes ~ 17 kDa of Acetylated Histone H3 at K9. It does not cross react to other modification or non-acetylated Histone H3 at Lysin 9.
• Purity/Purification: Purified by site-modified Epitope Affinity Purification.
• Form/Format: Supplied in sterile Phosphate buffered saline (pH7.2) containing antibody stabilizer.
• Sequence Length: 136

• Applicable Applications for anti-HIST1H3A antibody: ELISA (EIA), Flow Cytometry (FC/FACS), Immunohistochemistry (IHC), Immunoprecipitation (IP), Western Blot (WB)
• Application Notes: ELISA: 0.01-0.1 ug/ml; Flow Cytometry: 5-10 ug/ml; Immunohistochemistry: 2-10 ug/ml; Immunoprecipitation: 2-5 ug/ml; Western Blot: 0.1-1 ug/ml
• Preparation: A synthetic peptide containing TAR[Acetyl-K]STGG in which Acetylation at lysine 9 of human histone H3
• Preparation and Storage: The antibodies are stable for 12 months from date of receipt when stored at -20 degree C. The antibodies can be stored at 2 degree C - 8 degree C for one month without detectable loss of activity. Avoid repeated freezing-thawing cycles.

Related Product Information for anti-HIST1H3A antibody

Histones are basic nuclear proteins that together with DNA make up the nucleosome structure in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The methylation of specific residues in the histone tails is a central modification for regulatingepigenetic transitions in chromatin. Whereas methylation of histone H3 on lysine 4, 36, and 79 has been linked with gene activation, methylation of H3 on lysines 9, 27 and histone H4 on lysine 20 is associated with heterochromatin and some repressed genes with euchromatin. Modified lysine residues can exist in a mono-, di-, or tri-methylated state, while the arginine residues can be mono- or di-methylated. Histone H3 Lys4 trimethylation (H3-K4me3) is a conserved mark of actively transcribed chromatin. This antibody is specific for histone H3 tri-methylated at K4. The sequence is found in all mammals and a wide range of species, including D. melanogaster, Arabidopsis, Chicken and Xenopus. The antibody will react with any of the above species where the trimethylation modification is present.

References

Vakoc C et al. Histone H3 Lysine 9 Methylation and HP1gamma Are Associated with Transcription Elongation through Mammalian Chromatin. Molecular Cell (2003),  Volume 19,  Issue 3,  Pages 381 - 391.

NCBI and Uniprot Product Information

• NCBI GI #: 3273350
• NCBI GeneID: 8350
• Molecular Weight: 15,404 Da
• NCBI Official Full Name: histone H3
• NCBI Official Synonym Full Names: histone cluster 1 H3 family member a
• NCBI Official Symbol: HIST1H3A
• NCBI Official Synonym Symbols: H3/A; H3FA
• NCBI Protein Information: histone H3.1
• UniProt Protein Name: Histone H3.1
• Protein Family: Histone
• UniProt Gene Name: HIST1H3A
• UniProt Synonym Gene Names: H3FA

NCBI Description

Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. This structure consists of approximately 146 bp of DNA wrapped around a nucleosome, an octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6p22-p21.3. [provided by RefSeq, Aug 2015]

Uniprot Description

H3: Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. The nucleosome is a histone octamer containing two molecules each of H2A, H2B, H3 and H4 assembled in one H3-H4 heterotetramer and two H2A-H2B heterodimers. The octamer wraps approximately 147 bp of DNA. Belongs to the histone H3 family.

Protein type: DNA-binding

Chromosomal Location of Human Ortholog: 6p22.2

Cellular Component: extracellular region; membrane; nuclear chromosome; nuclear chromosome, telomeric region; nucleoplasm; nucleosome; nucleus; protein complex

Molecular Function: cadherin binding; histone binding; nucleosomal DNA binding; protein binding

Biological Process: blood coagulation; cellular protein metabolic process; chromatin silencing at rDNA; DNA replication-dependent nucleosome assembly; establishment and/or maintenance of chromatin architecture; negative regulation of gene expression, epigenetic; nucleosome assembly; positive regulation of gene expression, epigenetic; protein heterotetramerization; telomere organization and biogenesis