Immunofluorescence (IF)
(Immunofluorescence analysis of HeLa cells, using Histone H2B (Acetyl-Lys15) Antibody. The picture on the right is blocked with the synthesized peptide.)
Rabbit anti-Human, Mouse Histone H2B Polyclonal Antibody | anti-H2BFS antibody
Histone H2B (Acetyl-Lys15) Antibody
Reactivity
Human, Mouse
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, ELISA
Purity
Purified from rabbit antiserum by affinity-chromatography using acetylated peptide. The antibody against non-acetylated peptide was removed by chromatography using corresponding non-acetylated peptide.
Synonyms
Histone H2B; Polyclonal Antibody; Histone H2B (Acetyl-Lys15) Antibody; Histone cluster 1; H2bb; H2B.1; H2B/f; H2BFF; H2B histone family; member F; histone 1; anti-H2BFS antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Specificity
Histone H2B (Acetyl-Lys15) Antibody detects endogenous levels of total Histone H2B protein only when acetylated at Lys15.
Purity/Purification
Purified from rabbit antiserum by affinity-chromatography using acetylated peptide. The antibody against non-acetylated peptide was removed by chromatography using corresponding non-acetylated peptide.
Form/Format
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1 mg/ml (varies by lot)
Sequence Length
126
Applicable Applications for anti-H2BFS antibody
Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), ELISA (EIA)
Application Notes
WB: 1:500~1:1000
IHC: 1:50~1:100
IF: 1:100~1:500
ELISA: 1:1000
IHC: 1:50~1:100
IF: 1:100~1:500
ELISA: 1:1000
Target Modification
Acetyl
Modification Sites
H:K15; M:K15
Immunogen
The antiserum was produced against synthesized peptide derived from human Histone H2B around the acetylated site of Lys15.
Immunogen Range
Jan-50
Preparation and Storage
Stable at -20 degree C for at least 1 year.
Immunofluorescence (IF)
(Immunofluorescence analysis of HeLa cells, using Histone H2B (Acetyl-Lys15) Antibody. The picture on the right is blocked with the synthesized peptide.)
Immunofluorescence (IF)
(Immunofluorescence analysis of HeLa cells, using Histone H2B (Acetyl-Lys15) Antibody. The picture on the right is blocked with the synthesized peptide.)
Immunohistochemistry (IHC)
(Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue, using Histone H2B (Acetyl-Lys15) Antibody. The picture on the right is blocked with the synthesized peptide.)
Immunohistochemistry (IHC)
(Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue, using Histone H2B (Acetyl-Lys15) Antibody. The picture on the right is blocked with the synthesized peptide.)
Western Blot (WB)
(Western blot analysis of lysates from Raw264.7 cells, treated with TSA 400nM 24h, using Histone H2B (Acetyl-Lys15) Antibody. The lane on the right is blocked with the synthesized peptide.)
Western Blot (WB)
(Western blot analysis of lysates from Raw264.7 cells, treated with TSA 400nM 24h, using Histone H2B (Acetyl-Lys15) Antibody. The lane on the right is blocked with the synthesized peptide.)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
13 kDa
NCBI Official Full Name
histone H2B type F-S-like
NCBI Official Symbol
LOC102724334
NCBI Protein Information
histone H2B type F-S-like
UniProt Protein Name
Histone H2B type F-S
Protein Family
UniProt Gene Name
H2BFS
UniProt Synonym Gene Names
H2B/s
Uniprot Description
Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
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Product Notes
The H2BFS h2bfs (Catalog #AAA9507785) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Histone H2B (Acetyl-Lys15) Antibody reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's Histone H2B can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), ELISA (EIA). WB: 1:500~1:1000 IHC: 1:50~1:100 IF: 1:100~1:500 ELISA: 1:1000. Researchers should empirically determine the suitability of the H2BFS h2bfs for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Histone H2B, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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