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Western Blot (WB) (Western blot (WB) analysis of Histone H2B (Butyryl-K20) polyclonal antibody at 1:500 dilutionLaneA: HEK293T whole cell lysateLaneB: U20S whole cell lysateLaneC: DLD whole cell lysate)

Rabbit Histone H2B Polyclonal Antibody | anti-H2B antibody

Histone H2B (Butyryl-K20) polyclonal antibody

Gene Names
HIST1H2BB; H2B.1; H2B/f; H2BFF
Reactivity
Human, Mouse, Rat
Applications
Western Blot
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE)
Synonyms
Histone H2B; Polyclonal Antibody; Histone H2B (Butyryl-K20) polyclonal antibody; Hist2h2be; H2B 1A; H2B; H2B histone family; H2B2f; H2B2F; H2Ba; H2Bf; HIST2H2BF; histone H2B; histone H2B type 1; Histone H2B type 2-F; MGC131639; anti-H2B antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
Rabbit IgG
Specificity
Histone H2B (Butyryl-K20) polyclonal antibody detects endogenous levels of Histone H2B protein.
Purity/Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE)
Form/Format
PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Concentration
1mg/ml (varies by lot)
Applicable Applications for anti-H2B antibody
Western Blot (WB)
Application Notes
WB: 1:500~1:1000
IHC: 1:50~1:200
Immunogen
Synthetic peptide, corresponding to Human Histone H2B
Preparation and Storage
Store at 4 degree C short term. Aliquot and store at -20 degree C long term. Avoid freeze-thaw cycles.

Western Blot (WB)

(Western blot (WB) analysis of Histone H2B (Butyryl-K20) polyclonal antibody at 1:500 dilutionLaneA: HEK293T whole cell lysateLaneB: U20S whole cell lysateLaneC: DLD whole cell lysate)

Western Blot (WB) (Western blot (WB) analysis of Histone H2B (Butyryl-K20) polyclonal antibody at 1:500 dilutionLaneA: HEK293T whole cell lysateLaneB: U20S whole cell lysateLaneC: DLD whole cell lysate)
Related Product Information for anti-H2B antibody
Eukaryotic histones are basic and water soluble nuclear proteins that form hetero-octameric nucleosome particles by wrapping 146 base pairs of DNA in a left-handed super-helical turn sequentially to form chromosomal fiber. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form the octamer; formed of two H2A-H2B dimers and two H3-H4 dimers, forming two nearly symmetrical halves by tertiary structure. Over 80% of nucleosomes contain the linker Histone H1, derived from an intronless gene, that interacts with linker DNA between nucleosomes and mediates compaction into higher order chromatin. Histones are subject to posttranslational modification by enzymes primarily on their N-terminal tails, but also in their globular domains. Such modifications include methylation, citrullination, acetylation, phosphorylation, sumoylation, ubiquitination and ADP-ribosylation.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
13,950 Da
NCBI Official Full Name
histone H2B type 1-B
NCBI Official Synonym Full Names
histone cluster 1, H2bb
NCBI Official Symbol
HIST1H2BB
NCBI Official Synonym Symbols
H2B.1; H2B/f; H2BFF
NCBI Protein Information
histone H2B type 1-B; H2B histone family, member F; histone 1, H2bb; histone H2B.1; histone H2B.f
UniProt Protein Name
Histone H2B type 1-B
Protein Family
UniProt Gene Name
HIST1H2BB
UniProt Synonym Gene Names
H2BFF; H2B/f
UniProt Entry Name
H2B1B_HUMAN

NCBI Description

Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a member of the histone H2B family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6p22-p21.3. [provided by RefSeq, Jul 2008]

Uniprot Description

Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.

Research Articles on H2B

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Product Notes

The H2B hist1h2bb (Catalog #AAA3015109) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Histone H2B (Butyryl-K20) polyclonal antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's Histone H2B can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). WB: 1:500~1:1000 IHC: 1:50~1:200. Researchers should empirically determine the suitability of the H2B hist1h2bb for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Histone H2B, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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