Testing Data
(Recombinant Histone H2B (Lane 1) and 10ug acidextracted proteins from sodium butyrate treated HeLa cells (Lane 2) were resolved by electrophoresis, transferred to nitrocellulose and probed with MBS612758 H2B (1:40,000 dilution). Proteins were visualized using a goat anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system. Arrow indicates acetylated Histone H2B (15kD).)
Rabbit Histone H2B Polyclonal Antibody | anti-HIST3H2BB antibody
Histone H2B, acetylated (Lys5, Lys12, Lys15, Lys20) (MaxLight 750)
Gene Names
HIST3H2BB; H2Bb
Reactivity
Human, Bovine, Mouse, Rat
Applications
Immunoblot, Western Blot, Western Blot
Purity
Purified by Protein A Affinity Chromatography.
Synonyms
Histone H2B; Polyclonal Antibody; acetylated (Lys5; Lys12; Lys15; Lys20) (MaxLight 750); anti-HIST3H2BB antibody
Host
Rabbit
Reactivity
Human, Bovine, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Specificity
Recognizes acetylated Histone H2B, Mr ~15kD. Does not recognize acetylated Histone H2A or unacetylated recombinant Histone H2B protein. Species Crossreactivity: Human. Predicted to crossreact with mouse, rat and bovine based on sequence homology.
Purity/Purification
Purified by Protein A Affinity Chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight750.
Applicable Applications for anti-HIST3H2BB antibody
Immunoblot (IB), Western Blot (WB)
Application Notes
IB: 1:40,000-1:80,000 dilution of this lot detected acetylated Histone H2B in sodium butyrate treated, acid extracted proteins from HeLa cells. Unacetylated recombinant Histone H2B was not detected.
Applications are based on unconjugated antibody.
Applications are based on unconjugated antibody.
Immunogen
KLH-conjugated, synthetic peptide (...S[AcK]SAPAPK[AcK]GS[AcK]KAIT[AcK]AQ...) in which AcK corresponds to acetyl-lysine at residues 5, 12, 15 and 20 of human Histone H2B. The immunizing sequence has 24 out of 26 identical amino acids in mouse, rat and bovine.
Conjugate
MaxLight750
Antigen Modification
Acetylated
Note
Preservative Free
Preparation and Storage
Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight750 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Testing Data
(Recombinant Histone H2B (Lane 1) and 10ug acidextracted proteins from sodium butyrate treated HeLa cells (Lane 2) were resolved by electrophoresis, transferred to nitrocellulose and probed with MBS612758 H2B (1:40,000 dilution). Proteins were visualized using a goat anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system. Arrow indicates acetylated Histone H2B (15kD).)
Testing Data
(Recombinant Histone H2B (Lane 1) and 10ug acidextracted proteins from sodium butyrate treated HeLa cells (Lane 2) were resolved by electrophoresis, transferred to nitrocellulose and probed with MBS612758 H2B (1:40,000 dilution). Proteins were visualized using a goat anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system. Arrow indicates acetylated Histone H2B (15kD).)
Product Categories/Family for anti-HIST3H2BB antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
13,908 Da
NCBI Official Full Name
histone H2B type 3-B
NCBI Official Synonym Full Names
histone cluster 3, H2bb
NCBI Official Symbol
HIST3H2BB
NCBI Official Synonym Symbols
H2Bb
NCBI Protein Information
histone H2B type 3-B; H2B type 12; histone 3, H2bb
UniProt Protein Name
Histone H2B type 3-B
Protein Family
UniProt Gene Name
HIST3H2BB
UniProt Entry Name
H2B3B_HUMAN
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Product Notes
The HIST3H2BB hist3h2bb (Catalog #AAA6480579) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Histone H2B, acetylated (Lys5, Lys12, Lys15, Lys20) (MaxLight 750) reacts with Human, Bovine, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's Histone H2B can be used in a range of immunoassay formats including, but not limited to, Immunoblot (IB), Western Blot (WB). IB: 1:40,000-1:80,000 dilution of this lot detected acetylated Histone H2B in sodium butyrate treated, acid extracted proteins from HeLa cells. Unacetylated recombinant Histone H2B was not detected. Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the HIST3H2BB hist3h2bb for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Histone H2B, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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