Rabbit Histone H2AZ Polyclonal Antibody | anti-H2AZ antibody

Histone H2AZ Antibody

Cat. Num. AAA9613374

• Gene Names: H2AFZ; H2AZ; H2A.z; H2A/z; H2A.Z-1
• Reactivity: Human, Mouse, Rat; Predicted Reactivity: Bovine (100%), Sheep (100%), Dog (100%), Chicken (100%), Xenopus (91%)
• Applications: Western Blot, ELISA
• Purity: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin
• Synonyms: Histone H2AZ; Polyclonal Antibody; Histone H2AZ Antibody; H2A histone family member Z; H2A.z; H2A/z; H2afz; H2AZ; H2AZ_HUMAN; Histone H2A.Z; MGC117173; anti-H2AZ antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat; Predicted Reactivity: Bovine (100%), Sheep (100%), Dog (100%), Chicken (100%), Xenopus (91%)
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: Histone H2AZ Antibody detects endogenous levels of total Histone H2AZ.
• Purity/Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin
• Form/Format: Liquid. Rabbit IgG in phosphate buffered saline, pH7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1mg/ml (varies by lot)

• Applicable Applications for anti-H2AZ antibody: Western Blot (WB), Peptide ELISA (EIA)
• Application Notes: WB: 1:500-1:2000; Peptide ELISA: 1:20,000-1:40,000
• Immunogen: A synthesized peptide derived from human Histone H2AZ, corresponding to a region within N-terminal amino acids.
• Conjugation: Unconjugated
• Fragment: Fab fragment
• Post Translational Modifications: Monoubiquitination of Lys-122 gives a specific tag for epigenetic transcriptional repression. Acetylated on Lys-5, Lys-8, Lys-12 and Lys-14 by KAT2A; KAT2A is recruited by the XPC complex in absence of DNA damage. Acetylated on Lys-5, Lys-8 and Lys-12 during interphase; acetylation disappears at mitosis (By similarity).Monomethylated on Lys-5 and Lys-8 by SETD6. SETD6 predominantly methylates Lys-8, lys-5 being a possible secondary site.Not phosphorylated.
• Subunit Structure: The nucleosome is a histone octamer containing two molecules each of H2A, H2B, H3 and H4 assembled in one H3-H4 heterotetramer and two H2A-H2B heterodimers. The octamer wraps approximately 147 bp of DNA. H2A or its variant H2AZ1 forms a heterodimer with H2B. H2AZ1 interacts with INCENP (By similarity). Interacts (via M6 cassette) with ANP32E; leading to removal of H2A.Z/H2AZ1 from the nucleosome. Heterodimer H2BC11 and H2AZ1 interacts with VPS72 (via N-terminal domain). Interacts with PWWP2A. Interacts with FH (when phosphorylated by PRKDC).
• Similarity: Belongs to the histone H2A family.
• Subcellular Location: Nucleus. Chromosome.
• Preparation and Storage: Store at -20 degree C. Stable for 12 months from date of receipt.

Western Blot (WB)

(Western blot analysis of H2A.Z in lysates of HeLa  , using H2A.Z Antibody(AF4666).)

Related Product Information for anti-H2AZ antibody

Variant histone H2A which replaces conventional H2A in a subset of nucleosomes. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. May be involved in the formation of constitutive heterochromatin. May be required for chromosome segregation during cell division.

NCBI and Uniprot Product Information

• NCBI GI #: 4504255
• NCBI GeneID: 3015
• NCBI Accession #: NP_002097.1
• NCBI GenBank Nucleotide #: NM_002106.3
• UniProt Accession #: P0C0S5; P17317; Q6I9U0; B2RD56
• Molecular Weight: 13,553 Da
• NCBI Official Full Name: histone H2A.Z
• NCBI Official Synonym Full Names: H2A histone family, member Z
• NCBI Official Symbol: H2AFZ
• NCBI Official Synonym Symbols: H2AZ; H2A.z; H2A/z; H2A.Z-1
• NCBI Protein Information: histone H2A.Z; H2AZ histone
• UniProt Protein Name: Histone H2A.Z
• UniProt Gene Name: H2AFZ
• UniProt Synonym Gene Names: H2AZ; H2A/z
• UniProt Entry Name: H2AZ_HUMAN

NCBI Description

Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene encodes a replication-independent member of the histone H2A family that is distinct from other members of the family. Studies in mice have shown that this particular histone is required for embryonic development and indicate that lack of functional histone H2A leads to embryonic lethality. [provided by RefSeq, Jul 2008]

Uniprot Description

H2AZ: Variant histone H2A which replaces conventional H2A in a subset of nucleosomes. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. May be involved in the formation of constitutive heterochromatin. May be required for chromosome segregation during cell division. The nucleosome is a histone octamer containing two molecules each of H2A, H2B, H3 and H4 assembled in one H3-H4 heterotetramer and two H2A-H2B heterodimers. The octamer wraps approximately 147 bp of DNA. H2A or its variant H2AFZ forms an heterodimer with H2B. H2AFZ interacts with INCENP. Belongs to the histone H2A family.

Protein type: DNA-binding

Chromosomal Location of Human Ortholog: 4q24

Cellular Component: Barr body; nucleosome; nuclear heterochromatin; nucleus

Molecular Function: protein binding; nucleosomal DNA binding; chromatin DNA binding; protein heterodimerization activity

Biological Process: positive regulation of transcription from RNA polymerase II promoter