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Western Blot (WB) (Western blot detection of Histone H1.3 in C6,3T3,Hela cell lysates using Histone H1.3 Rabbit pAb(1:1000 diluted). Predicted band size:22kDa. Observed band size:30kDa.)

Rabbit anti-Human Histone H1.3 Polyclonal Antibody | anti-HIST1H1D antibody

Histone H1.3 Rabbit pAb

Gene Names
HIST1H1D; H1D; H1.3; H1F3; H1s-2
Reactivity
Human
Applications
Western Blot, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Flow Cytometry, Functional Assay
Purity
Affinity purified
Synonyms
Histone H1.3; Polyclonal Antibody; Histone H1.3 Rabbit pAb; anti-HIST1H1D antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
Rabbit IgG
Purity/Purification
Affinity purified
Form/Format
Supplied in 50nM Tris-Glycine (pH7.4), 0.15M Nacl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
Applicable Applications for anti-HIST1H1D antibody
Western Blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF), Flow Cytometry (FC/FACS)
Application Notes
WB: 1:1000
IHC: 1:200
ICC/IF: 1:100
FC/FACS: 1:20
Immunogen
A synthetic peptide of human Histone H1.3
Conjugation
Unconjugated
Modification
Unmodification
Subcellular Location
Chromosome, Nucleus
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.

Western Blot (WB)

(Western blot detection of Histone H1.3 in C6,3T3,Hela cell lysates using Histone H1.3 Rabbit pAb(1:1000 diluted). Predicted band size:22kDa. Observed band size:30kDa.)

Western Blot (WB) (Western blot detection of Histone H1.3 in C6,3T3,Hela cell lysates using Histone H1.3 Rabbit pAb(1:1000 diluted). Predicted band size:22kDa. Observed band size:30kDa.)
Related Product Information for anti-HIST1H1D antibody
Histone H1 protein binds to linker DNA between nucleosomes forming the macromolecular structure known as the chromatin fiber. Histones H1 are necessary for the condensation of nucleosome chains into higher-order structured fibers. Acts also as a regulator of individual gene transcription through chromatin remodeling, nucleosome spacing and DNA methylation.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
22,350 Da
NCBI Official Full Name
histone H1.3
NCBI Official Synonym Full Names
histone cluster 1 H1 family member d
NCBI Official Symbol
HIST1H1D
NCBI Official Synonym Symbols
H1D; H1.3; H1F3; H1s-2
NCBI Protein Information
histone H1.3
UniProt Protein Name
Histone H1.3
Protein Family
UniProt Gene Name
HIST1H1D
UniProt Synonym Gene Names
H1F3

NCBI Description

Histones are basic nuclear proteins responsible for nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H1 family. Transcripts from this gene lack polyA tails but instead contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6. [provided by RefSeq, Aug 2015]

Uniprot Description

Histone H1 protein binds to linker DNA between nucleosomes forming the macromolecular structure known as the chromatin fiber. Histones H1 are necessary for the condensation of nucleosome chains into higher-order structured fibers. Acts also as a regulator of individual gene transcription through chromatin remodeling, nucleosome spacing and DNA methylation ().

Research Articles on HIST1H1D

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Product Notes

The HIST1H1D hist1h1d (Catalog #AAA4752003) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Histone H1.3 Rabbit pAb reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Histone H1.3 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF), Flow Cytometry (FC/FACS). WB: 1:1000 IHC: 1:200 ICC/IF: 1:100 FC/FACS: 1:20. Researchers should empirically determine the suitability of the HIST1H1D hist1h1d for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Histone H1.3, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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