Rabbit anti-Human Histidine-rich Glycoprotein Polyclonal Antibody | anti-HRG antibody

Histidine-rich Glycoprotein, NT (HRG, HRGP, Histidine-proline-rich Glycoprotein, HPRG) (PE)

Cat. Num. AAA6480489

• Gene Names: HRG; HPRG; HRGP; THPH11
• Reactivity: Human
• Applications: Western Blot, ELISA
• Purity: Purified by Ammonium Sulfate Precipitation.
• Synonyms: Histidine-rich Glycoprotein; Polyclonal Antibody; NT (HRG; HRGP; Histidine-proline-rich Glycoprotein; HPRG) (PE); DKFZp779H1622; anti-HRG antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human HRG.
• Purity/Purification: Purified by Ammonium Sulfate Precipitation.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).

• Applicable Applications for anti-HRG antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:50-1:100; ELISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: Synthetic peptide selected from the N-terminal region of human HRG (KLH).
• Conjugate: PE
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western blot analysis of HRG antibody (N-term) in K562 cell line lysates (35ug/lane). HRG (arrow) was detected using the purified Pab.)

Immunohistochemistry (IHC)

(HRG Antibody (N-term) (RB18729) IHC analysis in formalin fixed and paraffin embedded human hepatocarcinoma tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the HRG Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.)

Product Categories/Family for anti-HRG antibody

Antibodies; Serum Proteins

References

1. Vanwildemeersch, M., Olsson, A.K. J. Biol. Chem. 281(15), 10298-10304 (2006). 2. Jones, A.L., Poon, I.K. J. Biol. Chem. 280(42), 35733-35741 (2005). 3. Hennis, B.C. and Kluft, C. Nucleic Acids Res. 19(15), 4311 (1991).

NCBI and Uniprot Product Information

• NCBI GI #: 123523
• NCBI GeneID: 3273
• UniProt Accession #: P04196; B9EK35; D3DNU7
• NCBI Official Full Name: Histidine-rich glycoprotein
• NCBI Official Synonym Full Names: histidine-rich glycoprotein
• NCBI Official Symbol: HRG
• NCBI Official Synonym Symbols: HPRG; HRGP; THPH11
• NCBI Protein Information: histidine-rich glycoprotein; histidine-proline-rich glycoprotein
• UniProt Protein Name: Histidine-rich glycoprotein
• Protein Family: Histidine-rich glycoprotein
• UniProt Gene Name: HRG
• UniProt Synonym Gene Names: HPRG

NCBI Description

This histidine-rich glycoprotein contains two cystatin-like domains and is located in plasma and platelets. The physiological function has not been determined but it is known that the protein binds heme, dyes and divalent metal ions. The encoded protein also has a peptide that displays antimicrobial activity against C. albicans, E. coli, S. aureus, P. aeruginosa, and E. faecalis. It can inhibit rosette formation and interacts with heparin, thrombospondin and plasminogen. Two of the protein's effects, the inhibition of fibrinolysis and the reduction of inhibition of coagulation, indicate a potential prothrombotic effect. Mutations in this gene lead to thrombophilia due to abnormal histidine-rich glycoprotein levels. [provided by RefSeq, Nov 2014]

Uniprot Description

Plasma glycoprotein that binds a number of ligands such as heme, heparin, heparan sulfate, thrombospondin, plasminogen, and divalent metal ions. Binds heparin and heparin/glycosaminoglycans in a zinc-dependent manner. Binds heparan sulfate on the surface of liver, lung, kidney and heart endothelial cells. Binds to N-sulfated polysaccharide chains on the surface of liver endothelial cells. Inhibits rosette formation. Acts as an adapter protein and is implicated in regulating many processes such as immune complex and pathogen clearance, cell chemotaxis, cell adhesion, angiogenesis, coagulation and fibrinolysis. Mediates clearance of necrotic cells through enhancing the phagocytosis of necrotic cells in a heparan sulfate-dependent pathway. This process can be regulated by the presence of certain HRG ligands such as heparin and zinc ions. Binds to IgG subclasses of immunoglobins containing kappa and lambda light chains with different affinities regulating their clearance and inhibiting the formation of insoluble immune complexes. Tethers plasminogen to the cell surface. Binds T-cells and alters the cell morphology. Modulates angiogenesis by blocking the CD6-mediated antiangiongenic effect of thrombospondins, THBS1 and THBS2. Acts as a regulator of the vascular endothelial growth factor (VEGF) signaling pathway; inhibits endothelial cell motility by reducing VEGF-induced complex formation between PXN/paxillin and ILK/integrin-linked protein kinase and by promoting inhibition of VEGF-induced tyrosine phosphorylation of focal adhesion kinases and alpha-actinins in endothelial cells. Also plays a role in the regulation of tumor angiogenesis and tumor immune surveillance. Normalizes tumor vessels and promotes antitumor immunity by polarizing tumor-associated macrophages, leading to decreased tumor growth and metastasis.