Rabbit anti-Human Histidine-rich Glycoprotein Polyclonal Antibody | anti-HRG antibody

Histidine-rich Glycoprotein, NT (HRG, HRGP, Histidine-proline-rich Glycoprotein, HPRG)

Cat. Num. AAA625795

• Gene Names: HRG; HPRG; HRGP; DKFZp779H1622
• Reactivity: Human
• Applications: ELISA, Western Blot
• Purity: Purified; Purified by ammonium sulfate precipitation.
• Synonyms: Histidine-rich Glycoprotein; Polyclonal Antibody; NT (HRG; HRGP; Histidine-proline-rich Glycoprotein; HPRG); Anti -Histidine-rich Glycoprotein; anti-HRG antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human HRG.
• Purity/Purification: Purified; Purified by ammonium sulfate precipitation.
• Form/Format: Supplied as a liquid in PBS, 0.09% sodium azide.

• Applicable Applications for anti-HRG antibody: ELISA (EL/EIA), Western Blot (WB)
• Application Notes: Suitable for use in ELISA and Western Blot.; Dilution: ELISA: 1:1,000; Western Blot: 1:50-1:100
• Immunogen: Synthetic peptide selected from the N-terminal region of human HRG (KLH).
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western blot analysis of HRG antibody (N-term) in K562 cell line lysates (35ug/lane). HRG (arrow) was detected using the purified Pab .)

Immunohistochemistry (IHC)

(HRG Antibody (N-term) (RB18729) IHC analysis in formalin fixed and paraffin embedded human hepatocarcinoma tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the HRG Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.)

Related Product Information for anti-HRG antibody

HRG contains two cystatin-like domains and is located in plasma and platelets. The physiological function has not been determined but it is known that the protein binds heme, dyes and divalent metal ions. It can inhibit rosette formation and interacts with heparin, thrombospondin and plasminogen. Two of the protein's effects, the inhibition of fibrinolysis and the reduction of inhibition of coagulation, indicate a potential prothrombotic effect. Mutations in this gene lead to thrombophilia due to abnormal histidine-rich glycoprotein levels.

Product Categories/Family for anti-HRG antibody

Antibodies; Abs to Serum Proteins

NCBI and Uniprot Product Information

• NCBI GI #: 2280514
• NCBI GeneID: 3273
• UniProt Accession #: P04196; B9EK35; D3DNU7; Q68DR3
• Molecular Weight: 59,578 Da
• NCBI Official Full Name: histidine-rich glycoprotein
• NCBI Official Synonym Full Names: histidine-rich glycoprotein
• NCBI Official Symbol: HRG
• NCBI Official Synonym Symbols: HPRG; HRGP; DKFZp779H1622
• NCBI Protein Information: histidine-rich glycoprotein; OTTHUMP00000210110; histidine-proline-rich glycoprotein
• UniProt Protein Name: Histidine-rich glycoprotein
• Protein Family: Histidine-rich glycoprotein
• UniProt Gene Name: HRG
• UniProt Entry Name: HRG_HUMAN

NCBI Description

This histidine-rich glycoprotein contains two cystatin-like domains and is located in plasma and platelets. The physiological function has not been determined but it is known that the protein binds heme, dyes and divalent metal ions. It can inhibit rosette formation and interacts with heparin, thrombospondin and plasminogen. Two of the protein's effects, the inhibition of fibrinolysis and the reduction of inhibition of coagulation, indicate a potential prothrombotic effect. Mutations in this gene lead to thrombophilia due to abnormal histidine-rich glycoprotein levels. [provided by RefSeq]

Uniprot Description

HRG: Plasma glycoprotein that binds a number of ligands such as heme, heparin, heparan sulfate, thrombospondin, plasminogen, and divalent metal ions. Binds heparin and heparin/glycosaminoglycans in a zinc-dependent manner. Binds heparan sulfate on the surface of liver, lung, kidney and heart endothelial cells. Binds to N-sulfated polysaccharide chains on the surface of liver endothelial cells. Inhibits rosette formation. Acts as an adapter protein and is implicated in regulating many processes such as immune complex and pathogen clearance, cell chemotaxis, cell adhesion, angiogenesis, coagulation and fibrinolysis. Mediates clearance of necrotic cells through enhancing the phagocytosis of necrotic cells in an heparan sulfate-dependent pathway. This process can be regulated by the presence of certain HRG ligands such as heparin and zinc ions. Binds to IgG subclasses of immunoglobins containing kappa and lambda light chains with different affinities regulating their clearance and inhibiting the formation of insoluble immune complexes. Tethers plasminogen to the cell surface. Binds T-cells and alters the cell morphology. Modulates angiogenesis by blocking the CD6-mediated antiangiongenic effect of thrombospondins, THBS1 and THBS2. Acts as a regulator of the vascular endothelial growth factor (VEGF) signaling pathway; inhibits endothelial cell motility by reducing VEGF-induced complex formation between PXN/paxillin and ILK/integrin-linked protein kinase and by promoting inhibition of VEGF-induced tyrosine phosphorylation of focal adhesion kinases and alpha-actinins in endothelial cells. Also plays a role in the regulation of tumor angiogenesis and tumor immune surveillance. Normalizes tumor vessels and promotes antitumor immunity by polarizing tumor-associated macrophages, leading to decreased tumor growth and metastasis. Defects in HRG are the cause of thrombophilia due to histidine-rich glycoprotein deficiency (THPH11). A hemostatic disorder characterized by a tendency to thrombosis.

Protein type: Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 3q27

Cellular Component: extracellular region; plasma membrane

Molecular Function: heparan sulfate proteoglycan binding; serine-type endopeptidase inhibitor activity; heparin binding; protein binding; zinc ion binding; metal ion binding; heme binding; immunoglobulin binding; cysteine protease inhibitor activity; receptor binding

Biological Process: platelet activation; positive regulation of apoptosis; positive regulation of immune response to tumor cell; negative regulation of cell adhesion; negative regulation of blood vessel endothelial cell migration; chemotaxis; response to organic cyclic substance; negative regulation of cell proliferation; negative regulation of angiogenesis; fibrinolysis; platelet degranulation; negative regulation of fibrinolysis; positive regulation of focal adhesion formation; regulation of gene expression; regulation of actin cytoskeleton organization and biogenesis; regulation of blood coagulation; regulation of protein complex assembly; angiogenesis; negative regulation of cell growth; blood coagulation; regulation of peptidyl-tyrosine phosphorylation; defense response to fungus; negative regulation of cell adhesion mediated by integrin

Disease: Thrombophilia Due To Histidine-rich Glycoprotein Deficiency