Rabbit Histamine H3 Receptor Polyclonal Antibody | anti-HRH3 antibody

Histamine H3 Receptor (HH3R, H3R, Histamine Receptor H3, HRH3, HGNC:5184, G Protein Coupled Receptor 97, GPCR97)

Cat. Num. AAA623963

• Gene Names: HRH3; HH3R; GPCR97
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot
• Purity: Affinity Purified; Purified by immunoaffinity chromatography.
• Synonyms: Histamine H3 Receptor; Polyclonal Antibody; Histamine H3 Receptor (HH3R; H3R; Histamine Receptor H3; HRH3; HGNC:5184; G Protein Coupled Receptor 97; GPCR97); Anti -Histamine H3 Receptor (HH3R; anti-HRH3 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human HRH3. Species Cross-Reactivity: mouse, rat.
• Purity/Purification: Affinity Purified; Purified by immunoaffinity chromatography.
• Form/Format: Supplied as a lyophilized powder from PBS, pH 7.4, 5% BSA, 0.05% thimerosal, 0.05% sodium azide. Reconstitute with sterile dH2O.

• Applicable Applications for anti-HRH3 antibody: Western Blot (WB)
• Application Notes: Suitable for use in Western Blot.; Dilution: Western Blot: 0.75ug/ml
• Immunogen: Synthetic peptide corresponding to a sequence at the C-terminal of human HRH3, different to the related rat sequence by 2aa.
• Preparation and Storage: Lyophilized powder may be stored at -20 degree C. Stable for 12 months at -20 degree C. Reconstitute with sterile dH2O. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Reconstituted product is stable for 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Related Product Information for anti-HRH3 antibody

The histamine receptor H3 (HRH3) is a presynaptic autoreceptor on histamine neurons in the brain and a presynaptic heteroreceptor in nonhistamine-containing neurons in both the central and peripheral nervous systems. The deduced 445aa HRH3 protein contains 7 predicted transmembrane domains. And it shares 22% and 21.4% aa sequence homology with the H1 (HRH1) and H2 (HRH2) receptors, respectively. The expression of recombinant HRH3 in a variety of cell lines conferred an ability to inhibit adenylate cyclase in response to histamine, but not to acetylcholine or any other biogenic amine. Additionally, HRH3 was most notably observed throughout the thalamus, the ventromedial hypothalamus, and the caudate nucleus. Strong expression was also seen in layers II, V,and VIb of the cerebral cortex, in the pyramidal layers of the hippocampus, and in olfactory tubercle.

Product Categories/Family for anti-HRH3 antibody

Antibodies; Abs to Receptors

NCBI and Uniprot Product Information

• NCBI GI #: 194018562
• NCBI GeneID: 11255
• NCBI Accession #: NP_009163.2
• NCBI GenBank Nucleotide #: NM_007232.2
• UniProt Accession #: Q548M6; Q8NCH4; Q8NI49; Q8NI50; Q8WXZ9; Q8WY00; Q8WY01; Q9Y5N1
• Molecular Weight: 48,657 Da
• NCBI Official Full Name: histamine H3 receptor
• NCBI Official Synonym Full Names: histamine receptor H3
• NCBI Official Symbol: HRH3
• NCBI Official Synonym Symbols: HH3R; GPCR97
• NCBI Protein Information: histamine H3 receptor; H3R; OTTHUMP00000031459; G protein-coupled receptor 97; G-protein coupled receptor 97
• UniProt Protein Name: Histamine H3 receptor isoform 1
• Protein Family: Histamine H3 receptor
• UniProt Gene Name: HRH3
• UniProt Entry Name: Q548M6_HUMAN

NCBI Description

Histamine is a ubiquitous messenger molecule released from mast cells, enterochromaffin-like cells, and neurons. Its various actions are mediated by histamine receptors H1, H2, H3 and H4. This gene encodes one of the histamine receptors (H3) which belongs to the family 1 of G protein-coupled receptors. It is an integral membrane protein and can regulate neurotransmitter release. This receptor can also increase voltage-dependent calcium current in smooth muscles and innervates the blood vessels and the heart in cardiovascular system. [provided by RefSeq]

Uniprot Description

H3R: The H3 subclass of histamine receptors could mediate the histamine signals in CNS and peripheral nervous system. Signals through the inhibition of adenylate cyclase and displays high constitutive activity (spontaneous activity in the absence of agonist). Agonist stimulation of isoform 3 niether modified adenylate cyclase activity nor induced intracellular calcium mobilization. Belongs to the G-protein coupled receptor 1 family. 7 isoforms of the human protein are produced by alternative splicing.

Protein type: Receptor, GPCR; Membrane protein, integral; GPCR, family 1; Membrane protein, multi-pass

Chromosomal Location of Human Ortholog: 20q13.33

Cellular Component: neuron projection; integral to plasma membrane; plasma membrane; myelin sheath

Molecular Function: drug binding; histamine receptor activity

Biological Process: negative regulation of glutamate secretion; eating behavior; neurotransmitter secretion; drinking behavior; negative regulation of gamma-aminobutyric acid secretion; negative regulation of adenylate cyclase activity; learning; regulation of norepinephrine secretion; response to organic cyclic substance; memory; G-protein signaling, coupled to cyclic nucleotide second messenger; negative regulation of serotonin secretion; elevation of cytosolic calcium ion concentration; negative regulation of blood pressure; brain development; positive regulation of epithelial cell proliferation