Rabbit HIST1H3A Polyclonal Antibody | anti-HIST1H3A antibody

HIST1H3A (Ab-27) Antibody

Cat. Num. AAA7106639

• Gene Names: HIST1H3A; H3/A; H3FA
• Reactivity: Human, Mouse, Rat
• Applications: ELISA, Western Blot, Immunohistochemistry, Immunofluorescence, Chromatin Immunoprecipitation, Immunoprecipitation
• Purity: Antigen Affinity Purified
• Synonyms: HIST1H3A; Polyclonal Antibody; HIST1H3A (Ab-27) Antibody; Histone H3.1; Histone H3/a; Histone H3/b; Histone H3/c; Histone H3/d; Histone H3/f; Histone H3/h; Histone H3/i; Histone H3/j; Histone H3/k; Histone H3/l; H3FA; AND; HIST1H3B; H3FL; HIST1H3C; H3FC; HIST1H3D; H3FB; HIST1H3E; H3FD; HIST1H3F; H3FI; HIST1H3G; H3FH; HIST1H3H; H3FK; HIST1H3I; H3FF; HIST1H3J; H3FJ; anti-HIST1H3A antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Antigen Affinity Purified
• Form/Format: Liquid
• Sequence Length: 136

• Applicable Applications for anti-HIST1H3A antibody: ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Chromatin Immunoprecipitation (ChIP)
• Application Notes: WB: 1:500-1:5000; IHC: 1:20-1:200; IF: 1:50-1:200
• Immunogen: Peptide sequence around site of Lys (27) derived from human Histone H3.1.
• Conjugation: Non-conjugated
• Buffer: Preservative: 0.03% Proclin 300; Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
• Preparation and Storage: Upon receipt, store at -20 degree C or -80 degree C. Avoid repeated freeze.

Western Blot (WB)

(Western BlotPositive WB detected in: NIH/3T3 cell acid extracts, Hela cell acid extracts, A549 cell acid extracts, Jurkat cell acid extracts, PC3 cell acid extracts, HEK293 cell acid extractsAll lanes: HIST1H3A antibody at 2ug/mlSecondaryGoat polyclonal to rabbit IgG at 1/50000 dilutionPredicted band size: 16 KDaObserved band size: 16 KDa)

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded human colon cancer using MBS7106639 at dilution of 1:100)

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded human lung cancer using MBS7106639 at dilution of 1:100)

Immunofluorescence (IF)

(Immunofluorescent analysis of Hela cells using MBS7106639 at a dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L))

Chromatin Immunoprecipitation (ChIP)

(Chromatin Immunoprecipitation Hela(4^106)were treated with Micrococcal Nuclease, sonicated, and immunoprecipitated with 8ug anti-HIST1H3A (MBS7106639)or a control normal rabbit IgG. The resulting ChIP DNA was quantified using real-time PCR with primers against the beta-Globin promoter.)

Product Categories/Family for anti-HIST1H3A antibody

Epigenetics and Nuclear Signaling

NCBI and Uniprot Product Information

• NCBI GI #: 4504281
• NCBI GeneID: 8350
• NCBI Accession #: NP_003520.1
• NCBI GenBank Nucleotide #: NM_003529.2
• UniProt Accession #: P68431; P02295; P02296; P16106; Q6ISV8; Q6NWP8; Q6NWP9; Q6NXU4; Q71DJ3; Q93081; A0PJT7; A5PLR1
• Molecular Weight: 15,404 Da
• NCBI Official Full Name: histone H3.1
• NCBI Official Synonym Full Names: histone cluster 1 H3 family member a
• NCBI Official Symbol: HIST1H3A
• NCBI Official Synonym Symbols: H3/A; H3FA
• NCBI Protein Information: histone H3.1
• UniProt Protein Name: Histone H3.1
• UniProt Gene Name: HIST1H3A
• UniProt Synonym Gene Names: H3FA

NCBI Description

Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. This structure consists of approximately 146 bp of DNA wrapped around a nucleosome, an octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6p22-p21.3. [provided by RefSeq, Aug 2015]

Uniprot Description

Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.